A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid

Answers

Answer 1

Answer:

tetracycline

Explanation:

When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while tetracycline gene is still intact.

- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.

- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.

- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.

Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.


Related Questions

What is the most common type of organic molecule?

protein

lipid

carbohydrate

carbon

Answers

Answer:

I am not certain but I think carbohydrate

Answer:

carbohydrate

Explanation:

carbohydrate is the most common type of organic molecule


Which describes a difference between bivalves and cephalopods?
Bivalves use tube feet for movement, and cephalopods use muscular feet for movement.
Bivalves live only in ocean environments, and cephalopods live in a variety of aquatic environments.
O Bivalves use a radula to feed, and cephalopods use filter-like structures to feed.
O Bivalves have an open circulatory system, and cephalopods have a closed circulatory system.

Answers

Answer: D - Bivalves have an open circulatory system, cephalopods have a closed circulatory system

Explanation: I had to research the differences to find the answer

A difference between bivalves and cephalopods are bivalve animals use a radula to feed, and cephalopods use filter-like structures to feed, that is the third option, where they both belong to the Mollusca phylum.

What is the significance of bivalves and cephalopods?

Both bivalves and cephalopods belong to the mollusca phylum, and they are invertebrates, some of the bivalves, such as oysters and mussels, are important as they are a source of food for humans and as filter-feeding organisms; at the same time, squid and octopus are cephalopods. These organisms are significant in different fields of study, such as neuroscience and evolution.

Hence, a difference between bivalves and cephalopods are bivalve animals use a radula to feed, and cephalopods use filter-like structures to feed, that is the third option, where they both belong to the Mollusca phylum.

Find out more about bivalves and cephalopods here.

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onsider a scenario where a native plant population is growing at it's carrying capacity in a community. The seed of an invasive species is brought in by a squirrel, which then germinates, grows and reproduces resulting in the establishment a population of this invasive plant species. The invasive species has very similar niche as the native plant species. How do you predict the invasive plant population to be affected by the presence of this native species

Answers

The given question is incomplete, the complete question is:

Consider a scenario where a native plant population is growing at it's carrying capacity in a community. The seed of an invasive species is brought in by a squirrel, which then germinates, grows and reproduces resulting in the establishment a population of this invasive plant species. The invasive species has very similar niche as the native plant species. How do you predict the native plant population to be affected by the presence of this invasive species?

The invasive species fitness will be limited by the native plant species

The native plant carrying capacity will go down, relative to that prior to the invasive species arrival

There will be no change to the native plant population

The invasive species will grow unchecked and exponentially

No listed answer is correct

Answer:

The correct statement is the fourth one, that is, the invasive species will grow exponentially and unchecked.

Explanation:

The fitness of the invasive species will not get restricted by the native species. Though the opposite takes place as the original niche of the local species does not possess the natural predator for the invasive species. Therefore, invasive species emerge fitter and reproduce exponentially without population control.

The carrying capacity of the native species will not go down before the arrival of the invasive species, however, will go down post its arrival.  

As the invasive species has no natural predators, one can witness a substantial reduction in the population of the local plant population. The invasive species will utilize the niche of the local plant, therefore, resulting in the reduction of the native species population, as they have share habitat, food resources, and other natural resources.  

It is correct that the invasive species will grow without any control. An outbreak of invasive species generally takes place due to the absence of its natural predators in the ecosystem at the time of its introduction. Hence, the correct statement is the fourth one.

Invasive species establish in a new area, overgrow, and negatively interact with native species. The correct option is  4). The invasive species will grow unchecked and exponentially

-----------------------------------

Let us firts reviw some framework.

When talking about dispersion, we refer to the change in a species' range or distribution area.

When species move and establish in a new area, out of their original distribution range, they might be considered exotic or invasive, according to their

reproductive rate,

population growth, and

interaction with native species.

The introduction of invasive species has ecological, economic, socio-cultural consequences.

Once an invasive species has been established, it overgrows.

In the new areas, these species have less environmental pressure and better conditions than in their origin area -fewer predators, more resources, better nitches-.

These factors favor their establishment because they are adapted to surviving under harder conditions.  

They interact with native species by competing for resources such -water, place, radiation, exposure, shelter, food, etcetera-.  

They can also become significant predators of native species, consuming a wide diversity of prey items, and in many cases, there are no native species that may prey on them.

They provoke significant damages in the structure of native ecosystems, causing severe damage in endemic species.

They produce ecological imbalances in the trophic chains and the dynamics of communities. Invasive species are uncontrollable.

As they expand, they can also affect human in an economic manner and has socio-cultural effects. Many invasive species might cause crops loss by eating cultures. They attack farm animals. They affect buildings and other structures or ships by eating wood. They might even attack humans and cause health problems.

According to this information, we can answer the question.

We need to predict how NATIVE species are affected by INVASIVE species.

The options are,

1) The invasive species fitness will be limited by the native plant species

When talking about invasive species, this is not an option. Invasive species always affect the fitness of native species. So this option is not correct.  

2) The native plant carrying capacity will go down, relative to that prior to the invasive species arrival

The carrying capacity will still be the same. But resources availability will decrease faster because both species have the same requirements. They compete for the same resources. This option is not correct.

3) There will be no change to the native plant population

The will occur changes for the native population for sure. It will be affected by the presence of invasive species. So this option is not correct.  

4) The invasive species will grow unchecked and exponentially

Invasive species population will express an uncontrol exponential growth during its establishment phase.

This plant species find the perfect conditions to reproduce and increase its population size.

It finds available space, no predators, water and nutrients availability, proper light exposure, among others.

They can successfully compete with native species, displacing them and getting more space.  

THIS IS THE CORRECT OPTION

5) No listed answer is correct

Not correct

---------------------------------------

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A "healthy ecosystem" can also be described as:

Answers

Answer:

water and rocks; energy source, break down dead plants and animals, returning vital nutrients to the soil.

Explanation:

Which organism is least related to the bird on this cladogram?

Answers

Answer:

SHARKS HAVE THE LEAST TRAITS IN COMMON WITH BIRDS.



I’ll give brainlist if it’s outstanding

How do parachutes work?

Two paragraphs

Use all these terms in your paragraph: Gravity, Acceleration, terminal velocity, force, air resistance.



Help plssss

Answers

Answer:

Parachutes are made of light but strong materials to keep yyouin the air and have a safe landing :)

Explanation:

How do abundant growth and care resources affect ecosystem?

Answers

Answer:

over exploitation

Explanation:

Can you see individual cell without a microscope?

Please help me

Answers

No you can’t because the object has to be at least 0.1 Millimeters long to be visible

Whats planets rotates in a reclining position?

Answers

Answer:

Jupiter. Mercury. Saturn

Explanation:

Why do girls get more girls? (genetically, not because "their girls and normally get long hair")

Answers

Answer:

wdym? I'm confused

Explanation:

Answer: girls have the gene to get pregnant and give birth

Explanation: what are you even asking

Are you talking about how girls get more babies or?

If yes then girls have the gene to get pregnant and give birth

What is the percent by mass of a solution that contains 3g of salt in 12 g of solution?

Answers

Answer:

25% salt

Explanation:

(3÷12)×100

=25%

In animals, respiration takes place through cellular interaction using ...__ *

Answers

Answer:

Cellular respiration?

Explanation:

The opening and closing of _____________in your heart create the lub-dub sound and prevent the backflow of blood.

What goes in the blank?

Answers

Answer:

Heart valves or the closure of the mitral and tricuspid atrioventricular (AV) valves at the beginning of ventricular systole and the closure of the aortic valve and pulmonary valve at the end of ventricular systole.

Explanation:

The heart tone “lub,” or S1, is caused by the closure of the mitral and tricuspid atrioventricular (AV) valves at the beginning of ventricular systole.

The heart tone “dub,” or S2 ( a combination of A2 and P2), is caused by the closure of the aortic valve and pulmonary valve at the end of ventricular systole.

what are the inputs for photosynthesis and where are they used?

Answers

Answer:

In terms of inputs, outputs and energy transformations, photosynthesis can be summarized as follows. Inputs - Sunlight as energy source, carbon dioxide and water. Processes - Chlorophyll traps sunlight; the energy is used to split water molecules; hydrogen from water is combined with carbon dioxide to produce glucose.

Explanation:

Which of the following is NOT part of the CIRCULATORY
system?

O artery
O capillary
O platelet
O alveoli

Answers

Answer::artery, atrium, blood vessel, capillary, circulatory system, heart,

Which of the following is an essential amino acid?

Answers

Answer:

The 9 essential amino acids are: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

Eukaryotic cells undergo cell reproduction by the process of mitosis. Which of the following accurately describes the importance of mitosis for an organism's survival?

Answers

Answer:

mitosis is very important for many organisms"s survival like starfish reform its cuted arm by mitosis and  bacterias reproduce by mitosis so that their population increases etc.

Explanation:

15. Explain how it would be possible to have a change in a single base of DNA, but have the protein NOT change and be functional.

Answers

Answer:

Nonsense mutation. A nonsense mutation is also a change in one DNA base pair. Instead of substituting one amino acid for another, however, the altered DNA sequence prematurely signals the cell to stop building a protein. This type of mutation results in a shortened protein that may function improperly or not at all.Sep 21, 2020

Explanation:

In the image of the food chain below the ____ is the producer.

A. Fungi

B. Hawk

C. Plants

D. Snake

Answers

Answer:

A

Explanation:Producers, also known as autotrophs, make their own food. SO its A

a
fungi is the answer

How might global climate change effect iguana populations?

Answers

Answer:

Since iguanas are cold blooded animals and can not regulate their own body temperatures, once temperatures reach 40° they become immobilized and they cant move, so if it is cold they will die.

Monomers linked together to form a polymer could be described as beads in a multicolored necklace. Which statement about monomers is true with respect to the above analogy?

A. Monomers in a polymer could be linked in various ways.
B. Monomers in a polymer are of different types.
C. Monomers in a polymer look different but are structurally similar.

Answers

The answer is B

Monomers linked together to form a polymer could be described as beads in a multicolored necklace because monomer in a polymer are of different types.

how does nature show emotion

Answers

Answer:Article

Authors

Metrics

Comments

Media Coverage

Abstract

Introduction

Study 1

Results

Discussion

Study 2

Results

Discussion

Study 3

Results

General discussion

Supporting information

Author Contributions

References

Reader Comments (0)

Media Coverage (0)

Figures

Abstract

Feeling connected to nature has been shown to be beneficial to wellbeing and pro-environmental behaviour. General nature contact and knowledge based activities are often used in an attempt to engage people with nature. However the specific routes to nature connectedness have not been examined systematically.

Explanation:

An explosion can quickly change chemical energy into:









sound, heat, and electricity O











light, electricity, and sound






light, heat, and electricity





sound, heat, and light​

Answers

Answer:

sound,heat,and electricity

Answer:

sound light and heat! it right im 100% sure

Explanation:

question 1. The construction of the Temple took seven years.
False True? question 2
Solomon sinned by allowing the corruption of God's people in the worship of idols.

True
False

Answers

1.) True .... 1 Kings 9:10 says it took Solomon 20 years altogether to build the Temple and his royal palace; the Temple itself was completed in 7 years

2.) True .... God hated it he would never have allowed it ....

Answer:

Question 1 true

question 2 true

Explanation:

Item 3
Which organ is found in the excretory system?


stomach


heart


bladder


large intestine

Answers

Answer:

Large intestine

Explanation:

Excretory Organs include- skin, liver, large intstine, lungs, and kidneys

Hope this helps

Answer:

skin, liver, large intestine, lungs, and kidneye

Explanation:

Google

What are the harmful effects of pteridophytes?​

Answers

These species can spread rapidly with negative consequences for native species. Invasive plant species have an impact on the diversity of local species, they affect water availability and damage the quality of soil nutrients. Once an alien plant has invaded a habitat, it changes the conditions of that environment.

Satellite imaging allows scientists to observe _______.
a.
where deforestation has removed the trees
b.
where deforestation has occurred but is recovering
c.
where deforestation has not occurred
d.
all of the above

Answers

Answer:

the answer is D. all of the above

Explanation:

i got 100% on my quiz :)

Satellite imaging allows scientists to observe where deforestation has removed the trees, where deforestation has occurred but is recovering, and where deforestation has not occurred. Thus, the correct option for this question is D, i.e. all of the above.

What is Deforestation?

Deforestation may be defined as the process of cutting down trees or the clearance of forests for the purpose of urbanization and industrialization. It can involve the conversion of forest land to farms, ranches, or urban use.

According to the context of this question, satellite imaging may be defined as a type of technology that gathers or collects information across the world with the help of satellites. The activity of deforestation is analyzed by this process along with recovering the surface.

Therefore, the correct option for this question is D, i.e. all of the above.

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20 points!!!!!!
Do prokaryotes have ribosomes? Why?

Answers

Answer:

yes they do because every cell needs ribosomes to manufacture proteins  

Answer:

yes because all the cells need ribosomes in order to make proteins

 

Explanation:

Which of the following statements about exponential growth curves is true?
- a. Exponential growth curves are common for R-selected species.
b. Exponential growth curves show fast growth rates followed by slow growth rates.
C. No organisms in nature experience exponential growth rates.
d Exponential growth curves are S-shaped.
Please select the best answer from the choices provided
B
ОООО

Answers

Answer:

Which of the following statements about exponential growth curves is true?

- a. Exponential growth curves are common for R-selected species.

Explanation:

The statement that is true about exponential growth curves is as follows:

Exponential growth curves are common for R-selected species.

Thus, the correct option for this question is A.

What is an Exponential growth curve?

An Exponential growth curve may be defined as a type of growth curve that significantly illustrates greater increases in the population of organisms with respect to time. It immediately represents the growth with extreme fast rates.

Exponential growth is followed by the continuous breeding population which contains more individuals than a similar population breeding annually. It includes examples like some insects, r-selected species, etc.

The shape of the exponential growth curve is J-shaped. It represents only fast growth rates. While the logistic growth curve shows fast growth rates followed by slow growth rates. It possesses a shape known as S-shape. It represents the growth rate of k-selected species.

Therefore, the correct option for this question is A.

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HURRY PLEASEEE. Photosynthesis and respiration are important because they produce or breakdown food to release energy. T F

Answers

True ✨✨✨✨✨✨✨✨✨!!!!!!!!!!!!
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