How is the PCR product separated from the PCR mixture at the completion of the reaction? A. Perform electrophoresis in an agarose gel, stain the gel and cut the band corresponding to the PCR product from the gel. B. Pour the PCR mixture into a commercially prepared DNA microconcentrator column and follow the manufacturer's directions to adhere and elute the PCR product from the column. C. Both of the above procedures may be used. D. Neither of the above procedures may be used.

Answers

Answer 1

The two suggested processes such as gel electrophoresis and micro concentrator column are applied. Therefore, choice C is right.

A specific piece of DNA can be quickly produced (amplified) in millions or billions of copies using the polymerase chain reaction, or PCR. It uses visual approaches based on size and charge to detect and identify gene sequences. The products of PCR are nothing but billion and millions of copies of target DNA or gene.

These genes are visualized by two techniques. One technique is agarose gel electrophoresis and another technique is the use of a micro concentrator column.

In agarose gel electrophoresis, the DNA samples are prepared and poured into the well. Then, they are separated based on size and charge using an electric field. Negatively charged DNA strands run toward the positive electrode and the smaller fragments run faster. In the micro concentrator column, the PCR products are poured according to the manual description and purified.

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Related Questions

Which mutation is harmful to the organism

A, a mutation allowing moths to camouflage better on blackened tree bark

B, a mutation making staphylococcus aureus resistant to the antibiotic methicillin

C, a mutation inhibiting human immunodeficiency virus from attaching to and entering the cell

D, a mutation causing uncontrolled cell division

E, a mutation giving plant leaves a bitter taste to discourage herbivores from eating them

Answers

Answer:

D,The Mutation causing uncontrolled cell division is the correct answer.

How many family members have dimples?

Please help!

Answers

Answer:

4

Explanation:

Shaded means that the family members are affected (which in this case means having dimples)

Answer:

5

Explanation:

Name two cord-mediated reflexes

Answers

Answer:

patellar, Achilles,

Explanation:

learned it in class

During what phase of meiosis does sister chromatids align at the equator?

Answers


During metaphase II, the centromeres of the paired chromatids align along the equatorial plate in both cells. Then in anaphase II, the chromosomes separate at the centromeres. The spindle fibers pull the separated chromosomes toward each pole of the cell.

A new human species was found on a distant planet, whose genetic inheritance and process of reproduction is identical to that of humans on earth, however, their phenotypes are much different. You are tracking two phenotypes in this new species: eye shape and tail size. The single gene that controls eye shape (gene E) is unlinked from the single gene that controls tail size (gene T). Square eyes is the dominant eye shape and round eyes is the recessive eye shape. Long tail is the dominant tail size and short tail is the recessive tail size.

Required:
An individual of this new species is heterozygous for gene E and heterozygous for gene T. What is their genotype and phenotype?

Answers

Answer:

The individual has the genotype Ee (for the eyes) and Tt (for the tail) and has square eyes and a long tail.

Explanation:

If an individual is heterozygous, it means that he has a dominant allele and a recessive allele for each genotype. In this case, if the individual is heterozygous for the E gene, he has the genotype formed with the "Ee" alleles. If the same individual is heterozygous for the T gene, it has the genotype formed by the "Tt" alleles.

Individuals with heterozygosis will present phenotypes related to the dominant allele, since the recessive allele is only expressed in hom0zygosis. In this case, the individual will have the phenotype square eyes and long tail.

Darla is a crime scene Investigator searching for latent bloodstains. She uses a chemical process that makes blood more visible, or darker, to the naked eye. Which chemical Is she using? Α) Luminol B) Fluoresceln C) Leuco Crystal Violet D) Ascetic acid​

Answers

Answer:

C

Explanation:

Leuco Crystal Violet.



The nerve impulses travel from the ear to the brain through the......

1)auditory nerve

2)factory nerve

3)facial nerve

4)optic nerve

5)auditory nerve



Answer and I will give you brainiliest

Answers

Answer:

1)Auditory nerve is answer.

Explanation:

Nerve impulses are transmitted from the ear to the brain via the auditory nerves.

I hope it's helpful!

When energy from the Sun reaches Earth, the energy is not absorbed at the same rate by the air,
the land or the surface. What does the uneven heating of the Earth cause?

Answers

Answer:uneven heating of Earth's surface produces wind because the air temperature and air pressure are different. The differences in the air causes the air to a lower pressure. ... Uneven heating of the Earth's surface produces air masses of different temperatures. Cold air sinks, forcing warm air to rise, hope this helps.

Explanation:

Osmosis is defined as movement of SOLVENT across a ___________ membrane

Answers

Answer:

Osmosis is defined as movement of SOLVENT across a semipermeable membrane.

Explanation:

Hope this helps!

Please help me ASAP please

Answers

Im pretty sure he is affected

Answer:

D

Explanation:

Because the grandparents are at the top he started it basically.

What do you mean by "in atleast 5 sentences" We were asked to write about typhoon Yolanda in atleast 5 sentences. So what do you mean by that?

Pls help.​

Answers

Answer:It means  in 5 sentences write about typhoon Yolanda

Explanation:

Enzymes can be regulated in a multitude of ways. One such way is by covalent modification, in which functional groups are attached to or removed from the enzyme. One such functional group that can be added to an enzyme is a phosphate group. Depending on the enzyme, addition of a phosphate group may increase or decrease that enzyme's activity. Which of the following is the general name of an enzyme that functions to add phosphate groups to its substrate?

a. Oxidoreductases
b. Ligases
c. Hydrolases
d. Isomerases
e. Transferases

Answers

Answer:

Option E

Explanation:

Transferase enzyme are able to transfer a molecule or atom from one compound to the other.  

For example – In the process of phosphorolysis,  add phosphate to the substrate group .  

X-Y + Z = X + Z-Y

Here Z in the phosphate group  

Hence, option E is correct

what is a natural resources ??​

Answers

Answer:

A natural resource is something found naturally that can be used by humanity to accomplish tasks. For example, wood is a natural resource as its found in nature and can be used by humanity for building and making fire for food and heat.

Explanation:

An earthquake is best described as a
I need an edg answer

Answers

Answer:

short term

Explanation:

Answer:

short term

Explanation:

please mark as brainliest

3+4-8×2 (4+2) answer of GEMDAS

Answers

Answer:

-89 is the answer

Explanation:

The answer: -89
Explanation: Use PEMDAS. So you would solve whats in the parentheses first, so 4+2 is 6. Then you do the multiplication so 8 times 2 is 16 and then 16 times 6 is 96. You are then left with 3+4-96. So you add the 3+4 and you are left with 7-96 which is -89.

When basaltic lava flows in channels or lava tubes it can move up to 30 km/hr (19 mi/hr). How would this type of flow affect the time it took the lava to reach the town?

Answers

Answer:

The time will be "2 hours".

Explanation:

Around 10 km/hr, lava eruptions were travelling. The vents was just 15 km (9.3 mi) away Kalapana city as well as 20 km (12.6 mi) from Pahoa city.  

So, we therefore need add the method, which would be: to taking out another time that civil defense officials just had to assess a city.

⇒  

Thus, to measure the time spent throughout the community of Kalapana,

⇒  

             

Which of the following is not a characteristic of a eukaryotic cell

Answers

Answer:

following means where man I can't understand sry for that

How can prey and predators be limiting factors for a population?
a. Unpredictable
b. Too many predators or too little prey can decrease a population
c. Too little predators or too many prey can decrease a population
d. Too many predators can limit a population
e. Predators and prey limit popuations

Answers

Answer:

b

Explanation:

your answer is B.

quick explanation: when there is the overpopulation of a predator species and there isnt enough prey for them to eat they risk extinction due to starvation.

Which of the following describes a lysosome

Answers

A tiny, membranous sac that contains enzymes that degrade worn cell parts and debris. ... the cell will swell and may eventually burst.

What organisms are capable of photosynthesis?
A. Plants only
B. Plants and algae only
C. Plants and some bacteria only
D. Plants, algae, and some bacteria

Answers

Answer:

D) Plants, algae, and some bacteria

Explanation:

Photosynthetic organisms include plants, algae, euglena and bacteria

What is the function of the phloem?

Answers

Answer:

The phloem transport food which is synthesized in leaves by the process of photosynthesis to the lower part of the tree or plant.

hope it helps

What is the phenotype and genotype?

Answers

Answer:

nwn . what's ?

lghtigrt ok njn

A phenotype is an observable characteristic , so it would be the fur we can see. the genotype is what we cannot see- the recessive white fur

How “Competition in an ecosystem” is playing a role in life?

Answers

Answer:

Competition is an interaction between organisms or species in which both the organisms or species are harmed. Limited supply of at least one resource (such as food, water, and territory) used by both can be a factor.

Explanation:

Which property of water allows it to dissolve substances like sodium Which property of water allows it to dissolve substances like sodium chloride and glucose but prevents it from dissolving lipids?

A. pH

B. polarity

C. adhesion

D. cohesion

Answers

It's polarity I think

Polarity is an intrinsic property of water associated with its behavior to dissolve substances like sodium chloride and glucose.

What is polarity?

It is a property of high importance because it defines the behavior of these related to other properties.

It represents the unequal electrical charges in the same molecule, said unequal splitting of electrons makes water a polar molecule.

Therefore, we can conclude that polarity is an intrinsic property of water associated with its behavior to dissolve substances like sodium chloride and glucose.

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77 POINTS, PLEASE HELP ASAP

1): Lily is dressing up as a chicken for Halloween this year and is flapping her arms like a bird as she walks from house to house. As Lily flaps her arms down to her sides, what type of movement is occurring?

2): Katie loves pretending that she’s a human cannonball. As she jumps off the diving board, she assumes the proper position before she pounds into the water: head and thighs tucked against her chest; back rounded; arms pressed against her sides while her forearms, crossed in front of her shins, hold her legs tightly folded against her chest. What movement term would best describe the positioning of Katie’s vertebral column, head, thighs, lower legs and lower arms? HINT: She is about as far from the anatomical position as she can get.

Answers

Answer:

1. abduction & abduction

2. flexed

) total suspended solids are mostly responsible for
a) turbidity b) colour c) odour d) taste

Answers

Answer:

I think the answer is colour

Which of the following distinguishes how auxins affect plant stems?


Auxins enable plant stems to search for water.

Auxins give plant stems their strength and rigidity.

Auxins enable plant stems to reach for sunlight.

Auxins give plant stems their green pigmentation.

Answers

Answer:

D.

Explanation:

Answer:

b

Explanation:

Auxins promote stem elongation, inhibit growth of lateral buds (maintains apical dominance). They are produced in the stem, buds, and root tips. ... This produces a curving of the plant stem tip toward the light, a plant movement known as phototropism. Auxin also plays a role in maintaining apical dominance.

Which statements describe a bacteriophage? Select all that Apply.

A. It is a type of virus that infects bacteria.

B. It is a type of bacteria that infects viruses

C. It is a virus consisting of a protein coat and a DNA core.

D. It is a bacterial strain consisting of protein and a DNA core.​

Answers

The answer is A because none of the rest apply to what a bacteriophage actually does

Answer:

The answer is A, and C.

Explanation:

Ducks are aquatic birds. Their feet are webbed and this trait makes them fast swimmers. Biologists believe that ducks evolved from land birds, which did not have webbed feet. How could this have happened

Answers

Answer:

Random chance mutations led to the webbed feet trait in ancestral land birds, conferring them an adaptive advantage. Birds that inherited the beneficial random mutations associated with the webbed feet trait had more chances to survive and reproduce, passing this trait to the next generations.

Explanation:

Mutations are ramdom changes in the nucleotide sequence of the genome of a particular organism. In general, mutations are deleterious, but mutations may occasionally be beneficial in certain environments. A beneficial mutation must be associated with one or more phenotypic traits capable of conferring an adaptive advantage in a certain environment, thereby the individuals carrying the mutation have more chances to reproduce and survive. In this case, one or more beneficial mutations associated with the webbed feet trait passed to the next generations, increasing their frequency in the population.

what are two good things that science has done for people, animals, or the earth

Answers

am i think amm people and animals but can be earth.

i think PEOPLE AND EARTH

Explanation:

people u can find things

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