How many copies of a protein need to be present
in a cell in order for it to be visible as a band on an SDS gel? Assume that you can load 100 µg of cell extract onto a gel and that you can detect 10 ng in a single band by sil- ver staining the gel. The concentration of protein in cells is about 200 mg/mL, and a typical mammalian cell has a volume of about 1000 µm³ and a typical bacterium a vol- ume of about 1 µm³. Given these parameters, calculate the number of copies of a 120-kd protein that would need to be present in a mammalian cell and in a bacterium in order to give a detectable band on a gel. You might try an order-of-magnitude guess before you make the calcula- tions.

Answers

Answer 1

In order for a protein to be visible as a band on an SDS gel, at least 1 x [tex]10^{15}[/tex] copies need to be present in a mammalian cell and 1 x [tex]10^{9}[/tex] copies need to be present in a bacterium.

Assuming that the molecular weight of the protein is 120 kDa, and we can load 100 µg of cell extract onto a gel and detect 10 ng in a single band, then we can detect 10/100000 µg of the protein, which is [tex]10^{-4}[/tex] µg.

To calculate the number of copies of the protein, we first need to determine how much of the protein is present in a cell.

For a mammalian cell with a volume of 1000 µm³, the total amount of protein is approximately 200 mg/mL x 1000 µm³ = 0.2 µg. For a bacterium with a volume of 1 µm³, the total amount of protein is approximately 200 mg/mL x 1 µm³ = 0.0002 µg.

Now, we can calculate the number of copies of the protein in a cell. For a mammalian cell, the number of copies is 0.2 µg / 120 kDa x 6.02 x [tex]10^{23}[/tex] molecules/mole = 1 x [tex]10^{15}[/tex] copies. For a bacterium, the number of copies is 0.0002 µg / 120 kDa x 6.02 x [tex]10^{23}[/tex] molecules/mole = 1 x [tex]10^{9}[/tex] copies.

Therefore, in order for a protein to be visible as a band on an SDS gel, at least 1 x [tex]10^{15}[/tex] copies need to be present in a mammalian cell and 1 x [tex]10^{9}[/tex] copies need to be present in a bacterium.

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Related Questions

How many alleles are typically found for mitochondrial genes?

1. 8

2. 1

3. 4

4. 2

Answers

Answer:

                     The answer is     2.1

Answer:

two alleles

Explanation: because This dynamic aspect of mitochondrial transmission from cell to cell is termed mitotic segregation. In Mendelian genetics, a person's nuclear DNA can contain, at most, only two alleles for any particular autosomal gene, one paternally derived (e.g., allele “A”) and the other maternally derived (e.g., allele “B”).

I hope this is what your looking for.

please help me with this!!!

Answers

Answer:

okkkkkkkkkkkkkkkkkkkkkkkkkkk 23.6/0987

Explanation:

This is easy (we just learned about this 1 day ago) it’s Tropo, Strat, Meso, then Thermo

Use your observations from the lab activity/lab video and the data tables to explain how salinity affects the way cold water from melting ice mixes with warm water.


MUST DO THE GIZMO LAB
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Answers

Answer:

The link doesn't work but it is known that when you add salt to ice, the ice not only melts but will actually get colder.

hope this at least helped you :>

Does mass alone determine whether an object will float or sink? Explain

Answers

Answer:

yes because if the mass is heavy it gonna sink, if its light its gonna float

Explanation:

There is balance between Apoptosis and Mitosis. What is the
characteristic of life that would involve maintaining this balance?
Metabolism
Respond to Stimuli
Homeostasis
Universal Genetic Code

Answers

Answer:

Homeostasis

Explanation:

What characteristic do sea stars and brittle stars have in common?

Both have flexible joints.
Both have teeth-like feeding structures.
Both use tube feet to catch prey.
Both have tentacles.

Answers

Answer:

They typically have a tough, spiny surface, which inspired their name (in Greek, echinos means “spiny” and derma means “skin”). They also have the unusual ability to regrow lost body parts, and sea stars and brittle stars can regrow arms if broken off or eaten

Explanation:

Sea stars and brittle stars both use tube feet to catch prey. Therefore, option C is correct.

What are sea stars and brittle stars?

Echinoderms with a star form that belongs to the class Asteroidea are known as starfish or sea stars. These terms are frequently used incorrectly to refer to ophiuroids, which are properly called brittle stars or basket stars in common usage. Due to their membership in the class Asteroidea, starfish are also referred to as asteroids.

The benthic (bottom-feeding) fauna of the maritime environment includes brittle stars. They belong to the Phylum Echinodermata, which includes marine organisms with radial symmetry, five components, and frequent spines. They are commonly referred to as echinoderms.

Sea stars and brittle stars both use tube feet to catch prey. Therefore, option C is correct.

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If one DNA strand reads CCGTAATGCAT, what will be the sequence of the complimentary strand?

Answers

The complimentary strand would be GGCATTACGTA

All levels of biological organization and discription?pllsss paki sagutan po​

Answers

The levels, from smallest to largest, are: molecule, cell, tissue, organ, organ system, organism, population, community, ecosystem, biosphere.

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Explain the importance/need for homeostasis.

Answers

Answer:

look

Explanation:

So ur body can work properly and without it you can die (: Hope I helped im not the best at this.

what do the arguments for and against the use of stem cells in medical research share?​

Answers

Answer:   Stem cell research can potentially help treat a range of medical problems. It could lead humanity closer to better treatment and possibly cure a number of diseases:

Parkinson’s Disease

Alzheimer’s Disease

Heart Diseases, Stroke and Diabetes (Type 1)

Birth Defects

Spinal Cord Injuries

Replace or Repair Damaged Organs

Reduced Risk of Transplantation (You could possibly get a copy of your own heart in a heart-transplantation in the future

Stem cells may play a major role in cancer

Better treatment of these diseases could also give significant social benefits for individuals and economic gains for society

Cons

"We should not mess with human life."

"Humans should not be trying to play God"

Some argue that stem cell research in the far future can lead to knowledge on how to clone humans. It is hard to say whether this is true, but we have seen devastating consequences of other research-programs, even with good intentions, such as nuclear research.

Stem cells are cells, which have the ability to transform into many different types of cells. The stems cells have the potential to specialize in any type.

The stem cells are extensively used for research, curing diseases, and especially in developing prosthetics. The stems cells provide new cells for the growth and development of the replaced and damaged cells.

The arguments for and against the use of stem cells are:

Arguments for the use of stem cells in medical research are:

1. The stem cells enhance the growth and development of healthy skin tissue.

2. The cells can be used to enhance the production of collagen.

3. The study of stem cells helps in understanding the causes of disease.

Arguments against the use of stem cells in medical research are:

1. The use of stem cells for research harms human life and dignity.

2. People believe that killing blastocysts or stem cells is equal to taking a human life.

3. However, the transplant of stem cells can be rejected by the patient's body, therefore, can cost the life of the patient.

Therefore, the use of stem cells for research can be fruitful or disadvantageous.

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Try to move the different parts of the body
by moving it back and forth, side to side,
rotating, and swinging.​

Answers

The given question is incomplete, however, the missing part is as follows:

body parts movement

neck _____

lower arm _______

upper arm ________

wrist __________

shoulder _________

skull _________

knee _________

hipbone _________

elbow _________

ankle _________​

Answer:

The correct answer is given as follows:

Explanation:

A. side to side

B. swinging

C. rotating

D. rotating

E. rotating

F. back and forth

G. swinging

H. side to side

I. back and forth

J. rotating

What provides the genetic material of an organism

Answers

Answer:

its parents.

Explanation:

Describe how Pangaea first split apart around 200 million years ago.

Answers

Answer:

Pangea was the world's supercontinent, with its splitting came preliminary continents of today.

Explanation:

The splitting occurred due to the movement of tectonic plates, where plates spread apart and moved the landmasses with it.

Which of the following statements is the of asexual reproduction?
A. It produces offspring
B. It produces genetically identical offspring
C. It produces genetic diversity
D. It produces offspring that are resistant to antibiotic.

Answers

The answer is B because the new cells formed during asexual reproduction are formed by mitosis there is no genetic diversity.

You do a serial dilution in the same manner as the one in the video. A plate with a sample volume of 10-6 mL produced 43 colonies. What was the original cell density (CFU)?

Answers

Answer: 43 x 10^6  CFU.

Explanation:

Plate count is a method used to determine the number of viable microorganisms in a sample. When the bacterial concentration is high, serial dilutions are carried out in a 1:10 sequence. Then, the dilutions are successive dilutions keeping the dilution factor constant at each step. Small aliquots of these dilutions are seeded in medium contained in a Petri dish where the bacteria grow forming colonies. Then the plates are incubated and colonies develop both within the agar and on the surface.

If the concentration of the seeded aliquot is too high, the bacteria will grow too much and it will not be possible to distinguish the colonies. However, if the concentration is very low, the number of colony forming units (CFU) may be very low and thus can be counted.

Colony forming units are calculated:

CFU = Number of colonies / (volume seeded on the plate * dilution).

The dilution is 10^-6, the number of colonies counted is 43, and usually the volume plated is 0.1 mL, so we replace that in the equation:

CFU= 43 / (0.1 mL * 10^-6)

CFU= 43000000 = 43 x 10^6

What caused the Kingdom of Life to change? Give 3 reasons minimum.​

Answers

Answer:

Due evolution, discoveries and having both characteristics.

Explanation:

The Kingdom of Life change due to evolution in the body of organisms, discoveries of new organisms that are different from plants and animals. There are some organisms that have both plant and animal characteristics so it can't be placed in plant or animal kingdom so that's why the kingdom of life changes from two kingdom system to 5 kingdom system.

Which event shows conditions that lead to natural selection?

A-Weeds are removed by a gardener from a public park

B-Wild horses are tamed over many years and selectively allowed to reproduce.

C-A crab produces millions of offspring in a year, but few survive to adulthood.

D-Farmers collect the largest corn seeds to plant in their fields the following year.​

Answers

Thy answer shall be C.

II. Which animal is the top predator in many wetlands?
A. Raccoons
B. Alligators
C. Snakes
D Owls

Answers

Answer:

Its B. Alligators. Yup they are the top predators

Hope this helped

All the best !!

Answer:

B alligators cause wet and crocodals are wet

In a food chain that includes, in order, plants, markhors, and snow leopards, what roles do markhors play? (Select all that apply.)

Answers

Answer:

-They are eaten by snow leopards

-They eat plants

Explanation:

Which of the following represents a cross between two heterozygous plants?
A. Ww x WW
B. ww x WW
C. Ww x Ww
D. WW x WW

Answers

c I hope it’s right

Can hydrogen peroxide be broken down by catalysts other than those found in a living system? What is/are the control(s) and why are they needed?

Answers

Answer:

hi I not talk spanish

Explanation:

not talk spanish


What are the three steps of the water cycle if the rain falls directly into the ocean?

Answers

Answer:

1. evaporation

2. condensation

3. precipitation

Explanation:

PHET skate park . Consider this equation: Kinetic Energy = ½ mv2. Which of those variables is changing as the skater moves up and down the track?

Answers

Velocity is the variable which is changing during motion of skater.

Impact of velocity on energy

Velocity is the variable which is changing when the skater moves up and down the track because moving upward on the slope decrease the speed of the skater while on the other hand, velocity increases when moving downward on the slope.

Mass of the body which is skating remain constant or can't change with height. Mass is the amount of matter present inside the body so we can conclude that velocity is the variable which is changing during motion of skater.

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The diploid cells of a radish plant contain 18 chromosomes. How many chromosomes are in a radish plant's haploid cells?

Answers

Answer:

9

Explanation:

The diploid chromosome number for a radish is 18 and the diploid chromosome number for cabbage is also 18. The haploid number for these species is nine. Therefore, the gametes of the radish and cabbage contain nine chromosomes.

In which soil horizon do we find organic material?

Answers

Answer:

We can find organic material in

Top soil

Hope it will help :)

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The process our cells use to convert broken down glucose into carbon dioxide (CO2) and water (H2O) is called what?

Question 3 options:

Aerobic Respiration


Anaerobic Respiration


Breathing Respiration


Cellular Respiration

Answers

Aerobic Respiration :))

Hope this helps

Answer:

Aerobic Respiration

Explanation:

i toke the quiz on K12 if itz rong im realy sorry.                      

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The amino
Each codon calls for one
acids are linked together by
It takes many amino acids to form a protein.
plz answer quick

Answers

Answer:

1. Proteins.

2. Peptide Bonds.

Explanation:

I majored in Biology

Find 2 ways that natural gas forms. List the steps of the two carbon pathways below. Label each location with A for atmosphere, B for the biosphere, G for geosphere, or H for hydrosphere. Use P for the anthroposphere.

Answers

Answer:

Biogenic and thermogenic

Explanation:

Biogenic and thermogenic are the two ways or mechanism that is responsible for formation of natural gas. Biogenic gas is formed with the help of methanogenic organisms that is present in marshes, bogs, landfills, and shallow sediments. Thermogenic gas is formed from organic matter that is buried in the deeper earth where the temperature and pressure are very high.

The organic pathway of the carbon cycle is the pathway in which carbon moves from atmosphere, through producers, herbivores and other organisms in the ecosystems, and converted back to the atmosphere.

In geological pathway, carbon moves from the atmosphere, through the ocean to rocks and the mantle, and then back to the atmosphere.

What is true about the insanity defense?

It is defined in the Diagnostic and Statistical Manual of Mental Disorders.
It can be used only if a person is diagnosed with a specific disorder from the DSM.
It is a legal term used to determine if a person can be held accountable for a crime.
It is a legal term that helps to determine if a person committed a crime or not.
It cannot be used if a person was previously found to be responsible for a different crime.

Answers

It is a legal term used to determine if a person can be held accountable for a crime

It's a legal term used to determine if a person can be held accountable for a crime. Hope this helps; have a wonderful day.

What are the 6 lobes of the brain and where are they located?

Answers

Frontal lobe.
Parietal lobe.
Occipital lobe.
Temporal lobe.
Limbic lobe.
Insular cortex. here is a photo (labeled )

Answer:

Frontal lobe.

Parietal lobe.

Occipital lobe.

Temporal lobe.

Limbic lobe.

Insular cortex.

The four lobes of the brain are the frontal, parietal, temporal, and occipital lobes (Figure 2). The frontal lobe is located in the forward part of the brain, extending back to a fissure known as the central sulcus. The frontal lobe is involved in reasoning, motor control, emotion, and language.

Explanation:

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Which of the following is the primary function of the juxtaglomerular complex?reabsorption of Na+ and other ionsconcentrating urinea system that protects the nephron from some chemicals found in bloodreleases chemical signals that regulate the rate of filtrate formation Determine each of these statements below can be defined as a 'True of Network Goods' or 'Not True of Network Goods.'A) The good with the highest quality always captures the majority of the market.B) Competition is for the entire market instead of within a market.C) Goods are usually priced above marginal cost.D) Network goods are always high tech.E) The utility of one consumer depends on the choices of other consumers.F) Firms that dominate the market for a network good often make it easy for consumers to switch between standards.G) The anti-trust implications of the network good model are the same as for natural monopolies.H) A single standard is often more profitable for all firms than dueling standards. 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