In a brief paragraph, explain how soluble proteins were obtained from fish muscle samples. Your explanation should include:
1. the purpose of mincing (cutting) and vortexing the tissue [2 pts,]
2. the purpose of the homogenization buffer [2 pts,] and
3. the purpose of the centrifugation step [2 pts.]
You must clearly describe what role each of these played in extracting and solubilizing protein from the samples to receive full credit. Revisit the "Micropipetting and Preparing Solutions" exercise to remind yourself of what the ingredients in the homogenization buffer were.

Answers

Answer 1

Answer:

Explanation:

This question is a practical question, however the processes involved in obtaining proteins after "sacrifice" is similar.

After removing the desired muscle sample from the fish during sacrifice, the muscle sample can be stored temporarily in a phosphate buffer saline which can also help remove blood and some other contaminants around the muscle sample. The sample can then be removed and weighed with the desired mass cut into small pieces in order to achieve a smaller surface area which assists for easy homogenization and buffer interaction. The homogenization buffer (which can be a phosphate buffer) is added to this small pieces and then "vortexed" so that the buffer can interact properly with the pieces. The purpose of the homogenization buffer is to prevent osmotic damage in the muscle cells while maintaining a constant pH (depending on the protein to be obtained). After homogenization with an homogenizer, the muscle cell membrane is ruptured and the soluble proteins dissolves into homogenization buffer (which must also be soluble to the desired protein) with a mass of suspended muscle tissues also in the buffer. This mixture is then centrifuged so as to obtain the dissolved/soluble protein (in the homogenization buffer) and separate it from the suspended masses in the mixture.


Related Questions

The body is supplied with oxygen by the _____.

respiratory system
circulatory system
endocrine system
excretory system
digestive system

Answers

the circulatory system is the answer :)

Which word describes a slope in biology?

Answers

Answer:

Concentration Gradient


Which one of the kingdoms is not characterized by eukaryotic cells

Answers

Answer:

Eukaryotic organisms that cannot be classified under the kingdoms Plantae, Animalia or Fungi are sometimes grouped in the kingdom Protista.

Explanation:

A person has floppy, unattached earlobes. This is a trait caused by a single gene (monogenetic). Having floppy earlobes is a dominant trait. If the letter E represents one allele, which of the following could be the genotype of this floppy-eared individual?

EE
Ee
ee
EE , Ee , and ee are all possible genotypes .
EE and Ee are possible genotypes , but not ee ..

Answers

It’s the fifth option! It could be EE or Ee but not ee.

At what site on the enzyme are reactants brought together
during a chemical reaction?

Answers

Explanation:

Enzymes bring reactants together so they don't have to expend energy moving about until they collide at random. Enzymes bind both reactant molecules (called the substrate), tightly and specifically, at a site on the enzyme molecule called the active site

The site on the enzyme at which reactants are brought together during a chemical reaction is known as the active site.

What are Enzymes?

Enzymes may be defined as biocatalysts that increase the rate of a chemical reaction without being consumed or changed in the overall process. It may be thought that all cellular reactions and processes are mediated by enzymes.

The construction of the enzyme-substrate complex is the first step in the mechanism of enzymatic catalysis. In this step, the reactant molecules are brought together during a chemical reaction. These reactant molecules bind to the active site of an enzyme.

The Active site of an enzyme is the region where substrates bind and catalysis initiates.

Therefore, the active site is the region on the enzyme at which reactants are brought together during a chemical reaction.

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Glucose enters the cell through which of the following mechanism?
Select one:
a. Facilitated diffusion
b. Central pathways
c. ATP binding cassette transport
d. Feeder pathways
e. Phosphotransferase system

Answers

Answer:

a. facilitated diffusion

Explanation:

Instead, cells assist glucose diffusion through facilitated diffusion and two types of active transport.

Which choice is correct?

Answers

Answer:

B is the correct answer

the answer is B glucose and fructose

true or false the offspring produced by asexual reproduction are genetically identical to their parents.

Answers

Answer:

True

Explanation:

Which process is NOT fueled by ATP produced in cellular respiration. First exclude the four processes that are fueled by ATP produced in cellular respiration. Which process is NOT fueled by ATP produced in cellular respiration. First exclude the four processes that are fueled by ATP produced in cellular respiration. building gradients of substances across the outer cell membrane movement of vesicles along cytoskeletal tracks transport of protons (H+) from low concentration in the mitochondrial matrix to high concentration in the mitochondrial intermembrane space chemical work phosphorylation of a motor protein in the cytosol

Answers

Answer:

transport of protons (H+) from low concentration in the mitochondrial matrix to high concentration in the mitochondrial intermembrane space

Explanation:

atpase pump can also be called atp synthase. this enzyme catalyses atp formation from adenosine diphosphate and phosphate. it has f1, stalk and f0 components. 3 positive hydrogen ions go through to make 1 adenosine triphosphate molecule. oxidative phosphorylation has to do with the loss of electrons. there would be electrons loss from NADH to FADH2. Cytochromes carries them through different series of transferases from I to IV and while on this positive hydrogen ions are released into mitochondrial matrix

positive hydrogen ions are moved back to lumen through adenosine triphosphate channels. a process called chemiosmosis. the pro

Answer:

The correct answer is "building gradients of substances across the outer cell membrane"

Explanation:

Building gradients of substances across the outer cell membrane is a form of diffusion in which substances move across cellular membranes based on there independent gradient. This form of movement is a type of passive transport because it does not require the use of ATP for transport. The building gradients of substances across the outer cell membrane requires diffusion and does not require the use of ATP.

Movement of vesicles along cytoskeletal tracks requires individual vesicles to be transported by there respective motor proteins along the cytoskeletal tracks. These motor proteins require ATP to move these vesicles. Also, the phosphorylation of a motor protein in the cytosol requires one phosphorus molecules from ATP.

Transport of protons (H+) from low concentration in the mitochondrial matrix to high concentration in the mitochondrial intermembrane space is a description of the "hydrogen pump" that occurs in the electron transport chain. When hydrogen ion pump occurs, the hydrogen ion gets flown back through a ATP molecule.

What in the cell carries genetic
information?

Answers

Answer:

chromosomes

Explanation:

Why do these scientists use bioluminescence in their “superbugs? In this particular lab, how can they tell if the cells aren’t alive anymore?

Answers

Answer:

Not entirely positive but I do know that bioluminescence is a natural substance found in many living creatures and it makes them glow. They could be testing products for ideas of what undiscovered life is on earth. But all I do know is bioluminescence is an ingredient that causes things to glow.

Explanation:

Bioluminescence refers to a living thing that produces and emits light. Glow-in-the-dark animals can be found in all marine ecosystems, including those at the ocean's surface and deepest depths.

What is bioluminescence?

Utilizing bioluminescent bacteria, they can swiftly determine the quantity of bacteria present and end the experiment much more quickly, resulting in less pain, a shorter experiment duration, and the usage of fewer animals.

Current applications of the bioluminescence reaction include gene assays, the detection of protein-protein interactions, high-throughput screening (HTS) for drug development, hygienic monitoring, the assessment of environmental contamination, and in vivo imaging of tiny mammals.

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The molecules that capture light
energy are called...
A. mitochondria.
B. lysosome.
C. pigments.

Answers

Answer:

C

Explanation:

thats the answer im done whit that

5 mixture can be found in kitchen​

Answers

Answer:

1)Oil and water

2)flour and butter

3)tea and sugar

4)baking powder

5)soda and lemon juice

Answer:

mixed nuts

cake mix

cookies

mixed chocolate

ketchup

Explanation:

because all these mixtures are mixed

Describe how DNA's structure helps it be copied during cell division.

Answers

Answer:

The two strands of the DNA double helix uncoil at a specific place termed the origin during separation. The strands are then primed for duplication by a combination of enzymes and proteins. Finally, the assembly of the new DNA strands is organized by a specific enzyme called DNA polymerase.

Explanation:

What is the experiment for iodine test on starch? Step by step.

Answers

Answer:

Explanation: SOLID SAMPLE :

1. Peel off the skin of any vegetables e.g. potato and fruits as these are often impermeable. Use a clean spatula to remove samples of powdered food. Avoid cross-contamination with other foods.

2. Add a few ( 2-3) drops of bench iodine solution potassium to a piece of solid food on a white tile.

3.Make observations.                                                  

                       LIQUID SAMPLE:

1. Add 10 cm3 of the liquid food sample to a clean, dry test tube.

2.Add about 5 drops of iodine solution to the test tube.

3.Note any colour changes.

4.To prepare a control, perform steps 1 -3 for de-ionized water.

How would a dislodged piece of plaque in the cardiovascular system most likely affect an otherwise healthy person?


A: The right pulmonary artery could become blocked, restricting blood flow to the right lung.

B: narrow region of the superior vena cava could become blocked, restricting blood flow to the heart.

C: smaller artery in a leg could become blocked, restricting blood flow to capillaries in the leg.

D: narrow vein in the brain could become blocked, restricting blood flow to capillaries in the brain.

You must explain your answer

Answers

Answer:

pulmonary artery could become blocked.

Explanation:

The right pulmonary artery could become blocked, restricting blood flow to the right lung. A narrow region of the superior vena cava could become blocked, restricting blood flow to the heart.

PLEASE HELP ILL GIVE BRAINLEST

Cyanobacteria, also known as blue-green algae, are a kind of bacteria found in many lakes. These organisms make their own food through photosynthesis. Small animals including mayfly larvae eat the cyanobacteria, and small fish such as yellow perch eat the larvae. The small fish provide food for larger fish, such as walleye.

Describe a model you could use to show the transfer of energy within the lake ecosystem. In your description of your model, make sure you include the position of each organism identified in the paragraph.

Answers

Answer:

Cyanobacteria are aquatic and photosynthetic, that is, they live in the water, and can manufacture their own food. Because they are bacteria, they are quite small and usually unicellular, though they often grow in colonies large enough to see. They have the distinction of being the oldest known fossils, more than 3.5 billion years old, in fact! It may surprise you then to know that the cyanobacteria are still around; they are one of the largest and most important groups of bacteria on earth.

Many Proterozoic oil deposits are attributed to the activity of cyanobacteria. They are also important providers of nitrogen fertilizer in the cultivation of rice and beans. The cyanobacteria have also been tremendously important in shaping the course of evolution and ecological change throughout earth's history. The oxygen atmosphere that we depend on was generated by numerous cyanobacteria during the Archaean and Proterozoic Eras. Before that time, the atmosphere had a very different chemistry, unsuitable for life as we know it today.

The other great contribution of the cyanobacteria is the origin of plants. The chloroplast with which plants make food for themselves is actually a cyanobacterium living within the plant's cells. Sometime in the late Proterozoic, or in the early Cambrian, cyanobacteria began to take up residence within certain eukaryote cells, making food for the eukaryote host in return for a home. This event is known as endosymbiosis, and is also the origin of the eukaryotic mitochondrion.

Because they are photosynthetic and aquatic, cyanobacteria are often called "blue-green algae". This name is convenient for talking about organisms in the water that make their own food, but does not reflect any relationship between the cyanobacteria and other organisms called algae. Cyanobacteria are relatives of the bacteria, not eukaryotes, and it is only the chloroplast in eukaryotic algae to which the cyanobacteria are related.

Explanation:

what can be used to detect fermentation in yeast ?

Answers

Answer:

methylene blue

I hope this help

Answer:

The rate of fermentation can be measured by placing a small amount of yeast and sugar solution in a fermentation tube.

Explanation:

Fermentation is a metabolic process that produces chemical changes in organic substrates through the action of enzymes

A plant has the genotype AArrSs. Cross out gene combinations that cannot be produced or are not normal in the haploid gametes of this plant: Aas ArS Ars arS aRs Arr AS AArrSs

Answers

Hey ARS is correct I did it in class

When is it possible to break even in an energy transformation

A: Always
B: Late phase
C: Early Phase
D: Never

Answers

D: Never is the correct answer

Which statement summarizes the relationship between chromosomes,DNA, and genes

Answers

Answer:

The accurate answer to this is:

D.Genes use DNA code to manufacture proteins for chromosomes.

Explanation:

Genes use DNA code to manufacture proteins for chromosomes.

What are chromosomes?

The thread-like chromosomes that convey the genomic information from cell to cell are formed of protein and a single DNA molecule.

Chromosomes are found in the nucleus of cells in both plants and animals, including humans. There are 46 chromosomes in all, including 22 pairs of numbered chromosomes (autosomes) and one pair of sex chromosomes (XX or XY).

Children receive half of their chromosomes from their mother and half from their father because each pair comprises two chromosomes, one from each parent.

Therefore, Genes use DNA code to manufacture proteins for chromosomes.

To learn more about chromosomes, refer to the link:

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Which living is most closely related to Cetaceans such as whales?

Answers

Answer:

All Whales and Dolphins are a member of the Even-toed Ungulates or Artiodactyla. The groups closest living relatives are Hippos, sharing an ancestor approximately 47 million years ago. Once upon time, it was thought that Whales and Dolphins were closely related to extinct land-dwelling carnivores.

Explanation:

which structure inside the cell holds the digestive enzymes that break down the carbohydrates

Answers

Answer:

The lysosome is an organelle that contains digestive enzymes and acts as the organelle-recycling facility of an animal cell. It breaks down old and unnecessary structures so their molecules can be reused. Lysosomes are part of the endomembrane system, and some vesicles that leave the Golgi are bound for the lysosome.

Explanation:

True or False: Most asteroids are found floating freely
throughout the solar system.

Answers

Answer:

True

Explanation:

As they revolve around the Sun in elliptical orbits, the asteroids also rotate, sometimes quite erratically, tumbling as they go. More than 150 asteroids are known to have a small companion moon (some have two moons).

pls mark brainliest if I'm correct

which of the following is NOT true about evolution? NO LINKS
A. it is necessary for a population to survive
B. it results from environmental changes
C. large populations are affected more than
small populations
D. individuals moving into or out of the
population cause evolution.

Answers

Answer:

A

Explanation:

It can help a population to survive but it is not necessary.

Choose all the right answers. Animals cells do not need:
a cell membrane
chloroplasts
a cell wall
a nucleus​

Answers

Answer:

chloroplasts and cell wall

Explanation:

These are only found in plant cells.

Which organic molecules are present in milk?

Answers

Answer:

Explanation:

The principal constituents of milk are water, fat, proteins, lactose (milk sugar) and minerals (salts). Milk also contains trace amounts of other substances such as pigments, enzymes, vitamins, phospholipids (substances with fatlike properties), and gases.

fats proteins and lactose

Mercury's iron core takes up a majority of the planet's radius,
which plays a role in the planet's density.
TRUE
FALSE

Answers

Answer:

I think false sorry if wrong

A researcher conducts an investigation on fiddler crabs to understand the importance of claw size in mating. Male fiddler crabs have one normal-sized claw and one giant claw that they wave to attract female fiddler crabs. The female fiddler crabs have two small, same-sized claws. The researcher places 15 male crabs with different sized claws into an enclosure with similar conditions similar to their natural habitat. The researcher then releases 15 female crabs into the enclosure. The graph shows the percentage of male crabs of different claw size that successfully mated for three successive trials.

Answers

Answer:

The Answer is B

Explanation:

I got it right on USA TEST PREP

Zebras spend a lot of time traveling in search of grass to eat. It is dark out, and this zebra is not eating. What is happening to the carbon in the air around the zebras and the grass nearby?

Answers

Answer:

The carbon is moving into the air

Explanation:

Since the zebra is not eating, the carbon is only moving into the air and not out of the air

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