Place the Polymerase Chain Reaction events listed below in the order in which they occur. - Double-stranded DNA is produced - DNA sample is heated - Test tube with DNA sample is placed in machine - Taq polymerase initiates DNA synthesis - DNA denatures

Answers

Answer 1

Answer:

1- Test tube with DNA sample is placed in machine

2- DNA sample is heated

3- DNA denatures

4- Taq polymerase initiates DNA synthesis

5- Double-stranded DNA is produced

Explanation:

The Polymerase Chain Reaction (PCR) is a technique widely used in molecular biology laboratories in order to produce many copies of a specific DNA sample. Thermocyclers are machines designed for a cyclic temperature change of the PCR. First, an initial denaturation step where DNA sample is heated to separate the double-stranded DNA into two single strands. Subsequently, 20-40 PCR cycles are repeated to produce millions of copies of a specific DNA sequence. There are three steps in each PCR cycle: 1-Denaturation to 94–98 °C (DNA strands are separated), 2-Annealing to 50–67 °C (primers bind to each DNA strand on the opposite ends of the DNA strands to be copied) and 3-Extension to 75–80 °C (Taq polymerase initiates the synthesis of complementary DNA strands).


Related Questions

V
Which list places different units of matter in the correct sequence from
largest to smallest?
O A. Human body, organs, molecules, atoms, neutrons
B. Human body, molecules, organs, atoms, neutrons
C. Human body, atoms, organs, molecules, neutrons
D. Human body, organs, atoms, molecules, neutrons

Answers

Answer: A

Explanation:

A list that places different units of matter in the correct sequence from largest to smallest is as follows:

The human body, organs, molecules, atoms, and neutrons.

Thus, the correct option for this question is A.

What is Matter?

A matter may be characterized as any substance that significantly possesses mass and takes up space by having volume. It is anything that physically takes up space and can be weighed. In other words, the matter has volume and mass. There are many different substances, or types of matter, in the universe.

The human body is a structure that consists of numerous body systems in order to perform multiple functions for their survival. These body systems may include various organs. Molecules are composed of atoms that have three subatomic particles like electrons, protons, and neutrons.

Therefore, the Human body, organs, molecules, atoms, and neutrons is on the list that places different units of matter in the correct sequence from largest to smallest. Thus, the correct option for this question is A.

To learn more about the Human body, refer to the link:

https://brainly.com/question/25149738

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Which statement best describes how organisms demonstrate the transformation of energy?


Light energy from the Sun is converted into mechanical energy in a rabbit, which is converted into thermal energy and dispersed into the atmosphere.


Light energy from the Sun is converted into chemical energy in a wildflower, which is converted into mechanical energy in a deer that eats the wildflower.


Light energy from the Sun is converted into mechanical energy in a deer, which produces waste that is absorbed by a wildflower to produce chemical energy.


Light energy from the Sun is converted into chemical energy in a mushroom, which is converted into thermal energy as the mushroom breaks down dead matter.

Answers

Answer:

C

Explanation:

I would Say that is your best answer

Answer:

A

Explanation:

Cars are powered by fossil fuels, which come from the remains of ancient living things that were buried and exposed to high temperature and pressure from Earth’s interior.

Which spheres are represented when fossil fuels form?

A- atmosphere and biosphere
B- biosphere and geosphere
C- cryosphere and atmosphere
D- geosphere and hydrosphere

Answers

Answer:B

Explanation:Ancient living things were part of the biosphere at one point and the geosphere has to do with all of the earth.

Answer: The answer is B- biosphere and geosphere

Explanation: I have my ways ;>

Predict the phenotype for expression of the E. coli trp operon enzymes for the following mutants: a) A to U mutation at position 48 of the trp operon leader RNA. b) Deletion from positions 114-121 of the trp operon leader RNA. Explain your predictions taking into account the alternate stem-loop structures of the leader RNA.

Answers

I think the answer is a I believe

LOTS OF POINTS!?!?!Where does the CO2 in the atmosphere come from?

Answers

Answer:

Carbon dioxide is added to the atmosphere naturally when organisms respire or decompose (decay), carbonate rocks are weathered, forest fires occur, and volcanoes erupt. Carbon dioxide is also added to the atmosphere through human activities, such as the burning of fossil fuels and forests and the production of cement.

Explanation:

Answer:   Carbon dioxide is added to the atmosphere naturally when organisms respire or decompose (decay), carbonate rocks are weathered, forest fires occur, and volcanoes erupt. Carbon dioxide is also added to the atmosphere through human activities, such as the burning of fossil fuels and forests and the production of cement.

Explanation:

Can water hurt an organism?
Explaing why not or how it can​

Answers

Answer:

Yes, it can hurt one depending on the species, such as land animals having to be in the water for too long, it may drown, but it takes a certain amount of time to do so which means that for the most part, it is harmless.

Hope this helps!

why is the small intestine important​

Answers

The small intestine carries out most of the digestive process, absorbing almost all of the nutrients you get from foods into your bloodstream. The walls of the small intestine make digestive juices, or enzymes, that work together with enzymes from the liver and pancreas to do this.

Question 10 ot 25
The practice of science can answer only scientific questions. And scientific
questions guide the design of investigations. What is usually true of the
possible answers to a scientific question?
OA. They can lead to additional questions about the phenomenon.
OB. They overturn an established scientific theory.
OC. They lead to the discovery of a new scientific law.
OD. They remove the need for further scientific inquiry.

Answers

Answer:

B. they can overturn. an established scientific theory

Answer:They can lead to additional questions about the phenomenon

Explanation:

PLEASE HELP!
(click on the picture for it to be clear)
have a blessed day.

Answers

Answer:

It should be the first answer choice

Explanation:

Determine which of the following choices is the best match for the questions that follow: A. Whole-organism Vaccine B. Subunit Vaccine C. Toxoid Vaccine D. Experimental Vaccine

Answers

Answer:

Experimental Vaccine Herpes Vaccine = D

Attenuated = B

Purified macromolecule Vaccine = B

Recombinant-Vector Vaccine  = A

Inactivated or Killed = C

Diphtheria Vaccine = A

Explanation:

A Vaccine is an organic arrangement that gives dynamic obtained invulnerability to a specific irresistible infection. A Vaccine ordinarily contains a specialist that takes after a sickness causing microorganism and is frequently produced using debilitated or executed types of the organism, its poisons, or one of its surface proteins. The specialist invigorates the body's safe framework to perceive the specialist as a danger, pulverize it, and to additionally perceive and decimate any of the microorganisms related with that specialist that it might experience later on.

Vaccines can be prophylactic (to forestall or enhance the impacts of a future contamination by a characteristic or "wild" microorganism), or therapeutic (to battle a sickness that has just happened, for example, cancer).

The administration of Vaccine is called vaccination. vaccination is the best strategy for forestalling irresistible sicknesses

An unknown pill was found in Anna's digestive system. To figure out the identity of the unknown pill you "performed" testing on the unknown pill and different known pills by adding a variety of chemicals to the pills and observing their reactions. By comparing the reactions of the unknown substances to the known substances, you were able to identify the unknown pill.



Match the part of the experiment with the correct term.

Independent Variable=
Dependant Variable-
Control=
Constant=
A. The known Substances
B. adding 3 drops to each chemical to each substance
C. The reactions to different chemicals
D. Identity of the pill

Answers

Answer:

Independent Variable= Identity of the pill

Dependent Variable = The reactions to different chemicals

Control= The known Substances

Constant= adding 3 drops to each chemical to each substance

all matter Mass and time that exists and has ever existed is contained in which of the following

the sun
the planets
the milky way
the universe​

Answers

Answer:

The Milky Way

Explanation:

The Milky Way not only contains mass and time but it contains all of the solar systems, dust, maybe a black hole, and a lot of stars which is matter. Milky Way is a galaxy in a universe that contains a lot of planets it also contains mass and time.

It hasn't been confirmed nor denied but it is known that the milky way contains dark matter which is another type of matter and mass.

Hope this helped :)  

Answer:

The Universe

Explanation:

just got done with the quiz

reactant product
If there are 4 grams of reactant, how many grams of product are produced by the chemical reaction?
es
A)
O grams
B)
2 grams
4 grams
D)
8 grams
Law of Conservation of Matt
SPS3 a conservation of Mass In Chemical Reactic

Answers

Answer:

4 grams

Explanation:

If in an experiment, we have 4g of reactants, the mass of the products will also be 4g.

This is in compliance with the law of conservation of mass in a chemical reaction.

According to this law,

  "in a chemical reaction, matter is neither created nor destroyed but changed from one form to another".

If:

      A + B →  C

         4g        4g

mass of A and B must be equal to the mass of the product according to the law of conservation of mass.

give any two medical uses of oxygen​

Answers

Explanation:

Medical oxygen is used to: ... restore tissue oxygen tension by improving oxygen availability in a wide range of conditions such as COPD, cyanosis, shock, severe hemorrhage, carbon monoxide poisoning, major trauma, cardiac/respiratory arrest. aid resuscitation.

this is you're answer please give me thanks

Answer:

   In cells, oxygen is needed for aerobic respiration, which allows for the extraction of energy from ingested foods. Thus supplemental oxygen at home and in hospitals is vital for those with breathing disorders such as emphysema.

   Compressed oxygen tanks are used by mountaineers at high elevations to counteract the decreased O2 pressure at these altitudes.

   Supplemental oxygen is needed for surgical patients intentionally rendered paralyzed for medical procedures, in which "heart-lung machines" keep their vital functions going.

   Oxygen can be used as a sterilizing agent to kill certain anaerobic bacteria that are killed by sufficient exposure to the gas.

Industrial Uses of Oxygen:

   Oxygen is needed for the reaction that converts carbon to carbon dioxide gas in steel working, which takes place under high temperatures in a blast furnace. The carbon dioxide produced allows for the reduction of iron oxides into more pure iron compounds.

   Oxygen is used in other applications involving metal and requiring high temperatures, such as welding torches.

Aerospace Uses of Oxygen:

   In liquid form, oxygen is used widely as an oxidizing agent for use in missiles and rockets, where it reacts with liquid hydrogen to produce the terrific thrust needed for take-off. Astronaut spacesuits include a nearly pure form of oxygen.

   Oxygen is used to degrade hydrocarbon compounds, which are broken apart by heating them. This is used to create combustion that usually liberates water and carbon dioxide, but can also produce the hydrocarbons acetylene, propylene and ethylene.

   Oxygen is used in sewage-treatment and water-purification plants. It is forced through water to increase the production of bacteria that metabolize waste products in the water.

   Oxygen gas (O2) is needed to produce energy in things not linked to an electrical supply of their own, such as generators and vehicles (e.g., ships, airplanes and cars).

What two substances among four major types of organic compounds are made by living things? A.carbohydrates
B.water
C.proteins
D.potassium

Answers

Answer:

The answer is "Carbohydrate" and "Protein"

Explanation:

A solid eating regimen incorporates protein and starches. Why? Since these mixes are two of the primary structure obstructs that make your body. You get these structure blocks from the food that you eat, and you utilize these structure squares to make the natural mixes important forever.

The principle substance parts of living beings are known as natural mixes. Natural mixes are atoms worked around the component carbon (C). Living things are comprised of exceptionally huge particles.

These huge particles are called macro-molecules since "macro" signifies large; they are made by more modest molecules holding together. Our body gets these more modest particles, the "building blocks" or monomers, of natural atoms from the food we eat.

Answer:

A and C

Explanation:

i took the quiz

Briefly explain why it is difficult to determine the age of our planet.

Answers

Answer:

It is difficult to determine the age of our planet because the time this accretion process took is not yet known and predictions from different accretion models range from a few million up to about 100 million years

LOTS OF POINTS!?!?!The first part is just the dissolving of the carbon dioxide and water from groundwater, cloud, or rain making Type Here(H2CO3). FILL IN THE TYPE HERE PART!?!?!?

Answers

what’s the question and what part ?

Question 11 of 30
What kind of energy powers photosynthesis?
O A. Solar energy
B. Kinetic energy
O C. Electrical energy
D. Chemical energy
SUBMI

Answers

Answer:

solar energy

Explanation:

solar energy

- What is meant by nosocomial infection?​

Answers

Answer:

Hi

Explanation:

A nosocomial infection is contracted because of an infection or toxin that exists in a certain location, such as a hospital. People now use nosocomial infections interchangeably with the terms health-care associated infections (HAIs) and hospital-acquired infections.

nosocomial infection is something toxic around the area that’s infected , places like the hospital, just areas that aren’t clean and have virus

Much of the United States' prairie today:
A) consists of farms and ranches.
B) has gone back to its original condition.
C) changed to desert.
D) has now become urban cities.sc​

Answers

D) has now become urban cities

Which layer is between the mesosphere and the inner core? (7 points)

a
Asthenosphere

b
Crust

c
Lithosphere

d
Outer Core

Answers

Answer:

i think its otter core im not sure

Explanation:

doing the test rn i will be back and say if its wrong or not

Answer:outer core

Explanation:

Help Plz! Will give brainliest and for a lot of POINTS!



Which type of feature forms suddenly where intense compression deforms the rock in an area
a. volcano
b. normal fault
c. folded layers
d. reverse fault

Answers

Answer:

option b normal fault

Explanation:

sorry if i' m wrong

Answer:

Its reverse fault

Explanation:

Assuming that the motion of the object remains the same, at what position will the object be when it had traveled for a total of 6 s?
A. 2 m
B. 0 m
C. 1 m
D. 5m

Answers

Answer:

B. 0 m

Explanation:

From the position - time graph, the object would have traveled a distance of 0m after 6s.

The graph shows a decrease in distance traveled with increasing time.

the y - axis is the position of the body the x - axis is the the time taken

If we trace up the time taken to the curve, the position will be 0m.

Therefore, the position of the body after 6s is at 0m.

Describe why trasnsitioning to a hydrogen economy has advantages.
PLEASE HELLPPPPP

Answers

Answer:

the benefit of using hydrogen combustion over fossil fuel combustion is that it releases fewer emissions-water is the only major byproduct. No carbon dioxide is emitted, and nitrogen oxides, produced by a reaction with the nitrogen in the air, can be significantly lower than with the combustion of fossil fuels.

Explanation:

shown above

The elimination of pollution caused by fossil fuels - When hydrogen is used in a fuel cell to create power, it is a completely clean technology. The only byproduct is water. ... Distributed production - Hydrogen can be produced anywhere that you have electricity and water.


Which is a viable way to combat the effects of drought on the population of Sub-Saharan Africa?
A)
seeding the clouds
B)
increasing irrigation
C. increasing livestock
D)
planting many trees
H

Answers

Answer:

i am not that smart but think it's C

Explanation:

which other senses used in string telephone other than hearing?

Answers

...... Touchchhhhhhh

Cells in many-celled organisms...

A. All look the same
b. All have the same structure
c. are often quite different from each other
d. are the same size in every part of the organism

Answers

Answer:

c. are often quite different from each other

Explanation:

Multicellular organisms - composed of several different cells: Cells in these organisms differ in relation to the function to be performed.

The cells in these organisms are growing, developing, and reproducing.

The cells differ in shape, size, and role they have in the organism.

In the group of multicellular organisms are animals (Animalia), plants (Plantae), and fungi (Fungi).

Cells in many-celled organisms are often quite different from each other. Therefore option C is correct.

In many-celled organisms, such as animals and plants, cells can be specialized for specific functions, leading to their differentiation and diversification.

Different types of cells have distinct structures and perform specific tasks within the organism.

For example, nerve cells (neurons) are specialized for transmitting electrical signals, muscle cells for contraction, and blood cells for carrying oxygen and nutrients.

This specialization allows for efficient division of labor, where each cell type contributes to the overall functioning of the organism.

The diversity of cell types and their functions is a fundamental aspect of the complex organization and functioning of multicellular organisms, contributing to their overall biological complexity and adaptability.

Therefore option C are often quite different from each other is correct.

Know more about nerve cells:

https://brainly.com/question/15642720

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using realistic science examples, explain how you would improve a cats litter box??

Answers

Answer:

Sir yes

Explanation:

Etc

Lots of points plz help

Answers

1. C
2. A
3. B
Hope this helps
i think it is c,b,a. can you please mark me the brainliest. have a great day and good luck with school

Which option supports the decision to file an Animal Waste Management Plan (AWMP) with the state in the following scenario?

Yolanda wants to open a small-scale dairy farm in New Jersey. She plans to have 10 cows and 8 goats.
Animal farming operations of any size must file an AWMP.
Any farm operation with more than 10 animals must file an AWMP.
Her acreage size makes it a feeding operation, requiring an AWMP.
The animals she has meet the minimum weight criterion for an AWMP.

Answers

Answer:

Her acreage size makes it a feeding operation, requiring an AWMP.

Explanation:

Animal Waste Management Plan (AWMP) is a plan filed by farm owners on how the intend to manage the wastes that would be generated in their various farm.

This plan is filed with the state management board and leads to periodic visit to the farm in-order to ensure that, the farm owners are sticking to the plans which they enumerated earlier on how they are going to manage those wastes

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PLZ HELP SIMPE TRUE OR FALSEThe tribes in Pontiac's Conspiracy came only from the south, near Georgiatrue or falseThe Stamp Act was the first tax to visibly affect all the coloniststrue or false The American College Testing Corporation has a test called the ACT that is used for admissions to college. The American College Testing Corporation reports that students who attend 2-year colleges in the Midwest United States have an average ACT score of 21.5, with a standard deviation of 3.17. The president of the Minneapolis Community College wonders if students who attend her college have a ACT scores that are significantly different from the ACT scores reported by the American College Teaching Corporation. The president would perform a ___________ test, and use a z-critical value of _____. NUMBER 6 ONLY. Please help. Only if you are SURE A company used straight-line depreciation for an item of equipment that cost $16,950, had a salvage value of $4,200, and had a five-year useful life. After depreciating the asset for three complete years, the salvage value was reduced to $1,860 and its total useful life was increased from 5 years to 6 years. Determine the amount of depreciation to be charged against the machine during each of the remaining years of its useful life: Rhombus JKLM with vertices J(-4,7), K(0,8), L(-1, 4), and M(-5,3): (x, y) ------> (x + 2, y - 2) . In the formula I=Prt, what does I stand for? (4, 3), (1, 6), i need to find the slope of these points. Correct the double negatives: Nobody had not toured the newspaper plant until now. The Opium Wars in China and the expedition of Commodore Matthew Perry to Japan resulted in (1) the economic isolation of China and Japan (2) an increase in Chinese influence in Asia (3) the beginning of democratic governments in China and Japan (4) an increase in Western trade and influence in Asia For questions 14-16 pick out the variables that will be needed to answer the following question: Do trees grow faster if it is wetter in the summer?14) What is the independent variable?a.) amount of rainb.) no rainfallc.) amount of growth15) What is the dependent variable?a.) amount of rainb.) no rainfallc.) amount of growth16) What is the control?a.) amount of rainb.) no rainfallc.) amount of growth If a borrower can afford to make monthly principal and interest payments of 1000 and the lender will make a 30 year loan at 5 1/2%, or a 20 year loan at 4 1/2% what is the largest loan this buyer can afford give answer fast I am thinking of a number, let's call it x . If I increase twice my number by 5, the result is the same as if I triple my number and subtract 1. Use the 4-step process to write and solve an equation to find my number. Make sure to answer in a sentence. Joe is self-employed in a store that has a rental value of $500 a month which he pays, but he can vacate the building without giving notice. His other expenses are $100 a month for maintenance. He makes $25,000 a year on net sales (total revenue minus the wholesale cost of the product). If he quit his job and worked the same number of hours elsewhere at a job he liked equally well, he estimates that he could make $20,000 a year. No one else can be hired to work in the store. Joe should It is impossible to say with the information given in the problem. quit his job. work part-time. keep the job. A package of 12 pencils costs 2.95.A binder costs 3.48.Penelope buys a package of pencils and a binder. She pays with a 10note.What is her change? An amusement park charges $6 to enter, and $3 per game. If you paid a total of $18, how many games did you play? least to greatest, 45%, .46, 11/25 please help me with all Does anyone know where i could watch the movelittle house: look back to yesterday i cant find it ANYWHERE!!!! In the Lewis structure of BF3, how many lone pairs surround the boron atom? NEED ASAPSelect one:a. 0b. 1c. 2d. 3