Place the steps of constructing a genomic library in order. I. Digest phage with restriction enzyme. II. Lyse cells of interest and precipitate DNA. III. Introduce constructs via transformation into competent E. coli cells. IV. Perform ligation reaction with genome fragments and phage. V. Digest genomic DNA with restriction enzyme.g

Answers

Answer 1

Answer:

The correct answer is -

II: lyse cells of interest, precipitate DNA

V. Digest (cut) genomic DNA with restriction enzyme

I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!

IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.

III Introduce constructs via transformation into competent E. coli cells.

Explanation:

The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.

The genomic library construction involved some major steps that are:

- Extract and purify DNA - lysing and separating the desired DNA of interest.

- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes

- cut phage (chromosome) with restriction enzyme

- Insert the fragments of DNA into vectors

- introduce these recombinant a host bacterium by transformation, creating a DNA library.


Related Questions

Give two examples of elevation

Answers

Answer:

♥️pls sana po nakakatulong♥️

A drought decreases the water level in Lake Winnipeg. The carrying capacity of the lake decreases.
Limiting factor?

Explain

Answers

Answer:

Water, fish

Explanation:

The limiting factors are the water of course and the organisms living in the lake.

A drought decreases the water level in Lake Winnipeg and the carrying capacity of the lake decreases. This is because it is:

Density Independent

When something is said to be density Independent with respect to ecology, it means that an external force is able to influence the volume of a habitat, notwithstanding the density.

As a result of this, this particular principle is in place here as a drought which is an external factor is able to affect the size of the water.

Read more here:

https://brainly.com/question/9513807


Matter is passed from the living part of an ecosystem to the non-living part when which of the following takes
place?

A. Plants absorb gases

B. Plants decompose

C. Animals eat plants

D. Animals drink water

Answers

C i believe if not B

Question 4
Which of the following is not considered hazardous waste?
A
dead batteries such as those used in flashlights and radios
B
rotting meat and other food waste
С
metals such as lead and mercury
D

Answers

b rotting meat and other food waste

Question:
The phenylketonuria occurs in 1 of 10,000 individuals in a population. It is
caused by an autosomal recessive allele. Calculate the frequency of heterozygotes
in the population.

Answers

Answer:

10000 divided by 1 and then that answer

. Students decide to remove the litter from an abandoned park and plant trees. Which of the following observations provides the best evidence that the action of the students has had a positive effect on the health of the park ecosystem?
A. The number of human visitors decreases.
B.The level of yearly precipitation increases.
C.The number and diversity of animals increases.
D.The average daily high temperature decreases.

Answers

Answer:

c

Explanation:

what happen to LH and FSH during ovulatory phase???​

Answers

Answer:

LH and FSH also trigger an increase in the production of the female hormone estrogen .as estrogen rise,like a switch, it turns offthe production of follicle stimulating hormone.

CAN SOMEONE HELPP ME :(

Answers

I think it could be the answer b

Define the following term:
1. Pinhole camera

2. Screen

Answers

Pinhole camera: a camera with a pinhole aperture and no lens

Screen:a fixed or movable upright partition used to divide a room, give shelter from drafts, heat, or light, or to provide concealment or privacy.

How do we know that height in humans
is a polygenic trait and not a single-gene
trait?

A. There is no way to know for sure.

B. People have varying heights.

C. Height is too complex to be controlled by a single gene.

Answers

C.........................

GIVING BRAINLIEST ANSWER CORRECTLY FOR 40 POINTS
List 3 people from the history of biotechnology and write one sentence about each persons contribution​

Answers

Answer:

Karl Ereky

Explanation:

He discovered it

. Which of the following would you expect to have cells with a cell membrane? *
1 point
panda bears
pine trees
spiders
all of the above

Answers

Answer:

All of the above!

Explanation:

I'm just sure it's that.

Answer:

The answer is all of the above

Explanation:

Both plant and animal cells have a cell membrane.

what is self-imcompatibilty???​

Answers

Explanation:

Hope it will help you to solve your question.

Answer:

hiiiiiiiiiiiiiiiiiiii

Explanation:

understand

Which of the following correctly describes a cell that is haploid. There is more then one correct answer.


A cell with a single set of chromosomes

Includes zygotes

In humans contains 46 chromosomes

In humans contains 23 chromosomes

All cells except gametes

Includes gametes

A cell with two sets of chromosomes

Answers

Explanation:

a cell with a single set of chromosomes

in humans contain 23 chromosomes

includes gametes

the distance covered by a bus is measured by an instrument called​

Answers

Answer:

Odometer

It is measured by an instrument called odometer.

Proteins on the surface of a human cell and on a bird influenza virus are represented in the diagram below. Describe a change in the bird influenza virus that would allow it to infect this human cell.

Answers

Answer:

Viral proteins can modify the most superficial portion in such a way that it is complementary to the receptor of the human cell.

Generally this happens due to a genetic recombination between an avian virus and a human virus within the respiratory epithelium of the pig: this process underlies the emergence of new, more virulent strains of the influenza virus and underlies the emergence of influenza pandemics

Do you think humans would be
as dominant if the dinosaurs
were still present?

Answers

Answer:

Probably not, as we might have evolved to become prey instead of predator

Answer:

i dont think so.

Explanation:

the dinosaurs would've already ate us, and crushed the cities. dinosaurs are too dangerous for us. especially the T-rex.

Similarities between tridax plant and elephant grass

Answers

Answer:

ok so basically they just

How are taxonomic keys used?

Answers

Answer:

A taxonomic key is a simple tool used to identify a specific object. A taxonomic key is one of the most useful tools available to scientists trying to identify an unknown organism. Systematists rely on keys to help identify known organisms and determine whether they have discovered a new organism entirely.

Explanation: Hope this helps

Describe three ways in which economies are
likely to be affected by climate change

Answers

Answer:

1. Climate change affect the geography within which economy of the world operate

2. It changes the comfortability of human within the global industry

3. Growth Zone is affected

Explanation:

1. Climate change affect the geography within which economy of the world operate: economic of the world are affected by change in climatic condition in the since that there is shift in the usual period of seasons. For example, taken Nigeria as a region in West Africa, rainfall is expected to start around March in almost all part of the country, however, due to climate change, some part don't still have rain till May. This causes a shift in farmers plan and affect the supply of farm produce to the local market and to the global market, in turn affecting the gross domestic product of the country.

2. It changes the comfortability of human within the global industry. Take for example, fossil fuel, it is part of human being to address some unfavourable conditions among which is climate change which causes global warming, during this period, the way industries and people uses fossil fuel are been changed and it have direct effect to global economy.

3. Growth Zone is affected: change in climatic condition changes world economy growth zones. Thus could be seen in transportation sector, when the water level is affected, international movement of cargoes by ship are affect and hence, some trades agreements are altered thereby changing the growth zone.

A researcher exposes a bacterial culture to UV radiation and then subjects it to progressively higher temperatures. As the temperature increases, the bacteria have a progressively harder time surviving. Analysis of the dead bacteria reveals large amounts of misfolded, aggregated proteins compared to wild-type control bacteria exposed to the same temperature increase. Which of the following is a plausible explanation for the data?
A. The ribosomes are mutated and are non-functional.
B. The initiator tRNA (fMet) has been mutated and is non-functional.
C. Molecular chaperones have been mutated.
D. DNA polymerase is mutated and non-functional.

Answers

Answer:

C. Molecular chaperones have been mutated.

Explanation:

In prokaryotes; transcription and translation occur consecutively. Protein doubling additionally happens following the translation. The heat stun proteins or molecular chaperones are imperative to assume a part in the appropriate collapsing of the protein. At high-ranging temperatures, the protein misfolding can be ascribed to a change in mutation of the molecular chaperones.

Check all of the following statements that represent an example of predation. Check All That Apply A deer eats the leaves of a tree.A deer eats the leaves of a tree. Barnacles grow on various marine animals.Barnacles grow on various marine animals. A snail eats a clam.A snail eats a clam. A fungus decomposes dead organic matter.A fungus decomposes dead organic matter. A tree absorbs inorganic nutrients from the soil.A tree absorbs inorganic nutrients from the soil.

Answers

Answer:

- A deer eats the leaves of a tree.

- A snail eats a clam

Explanation:

Predation is one of the mode of feedings used by certain organisms. It is a relationship between two organisms in which one of the organisms called PREDATOR kills/harms and feed on the other organism called PREY.

According to this question, the two examples that represent predation are as follows:

- A deer eats the leaves of a tree; the deer, which is the PREDATOR feeds on a tree plant, which is the PREY.

- A snail eats a clam; the snail is the PREDATOR in this case and it harms the clam (PREY) by killing and feeding on it.

Answer:

A deer eats the leaves of a tree.

A snail eats a clam

Explanation:

E2023

Why do large islands usually have more species than smaller islands?

Answers

Answer: Reason Why

Explanation: A bigger target is easier to hit than a small one, and a big island is more likely to have species land on it by chance than a small one is. Larger islands have more space than smaller islands, so there are likely to be more resources available for species to use. The opposite is true for smaller islands.

In 1847, the German biologist Christian Bergmann noted that mammals and birds living at higher latitudes (farther from the equator) are on average larger and bulkier than related species found at lower latitudes. Suggest an evolutionary hypothesis to explain this observation.

Answers

Answer:

not quite sure how to do the hypothesis but its likely to be something to do with the fact that they need more fat and muscle due to the thinner atmosphere as respiration is harder to occur.

Explanation:

how many degrees in a triangle ​

Answers

Answer:

The internal degrees in a triangle add up to 180

Explanation:

Answer:

Three are three angles in a triangle which all add up to exactly 180 degrees

Explanation:

Question 7
5
Parents are exposed to hazardous chemicals. How might this affect their
future offspring?
The chemicals could make the parents sick before they have offspring,
The offspring could inherit damaged DNA from the parents.
The offspring could have red hair

Answers

Answer:

The offsprings could inherit damaged DNA from the parents.

Explanation:

The hazardous chemicals may cause mutations to the DNA.

Explain why a person with brain damage may be able to see perfectly well, but not recognize faces.​

Answers

Answer: Some people with a brain injury acquire a condition known as prosopagnosia(pronounced pro-so-pag-no-sia), or ‘face blindness’. People with the condition cannot tellthe difference between faces, an ability most of us take for granted. They may not evenrecognise the faces of their closest friends and family, or their own face in the mirror.

The function of the hepatic portal circulation is to ________. The function of the hepatic portal circulation is to ________. return glucose to the general circulation when blood sugar is low carry toxins to the kidney for disposal through the urinary tract distribute hormones throughout the body collect absorbed nutrients for metabolic processing in the liver

Answers

Answer:

The correct answer is ''collect absorbed nutrients for metabolic processing in the liver.''

Explanation:

Portal circulation is called that system that is between two large capillary beds, represented by the gastrointestinal organs, pancreas and spleen on the one hand, and the liver on the other. Hence, any venous system of the organism that is located between two capillary territories is called "portal system".The hepatic portal vein collects the deoxygenated blood from the gastrointestinal system, spleen and pancreas, this blood is venous and comes deoxygenated, in addition to being loaded with the nutrients absorbed in the digestive process, so it is a blood subjected to a high overload, but that quickly, thanks to its distribution through the liver, it will be bypassed to various chemical processes to extract a large part of the nutrients from it, and purify and eliminate toxins.

The function of the hepatic portal circulation is to collect absorbed nutrients for metabolic processing in the liver. Therefore, option D is correct.

The hepatic portal system is also known as the hepatic portal circulation or portal venous system. It is a specialized network of blood vessels that carries blood from the gastrointestinal tract, spleen, and pancreas to the liver. It plays a crucial role in directing nutrient-rich blood from the digestive organs to the liver for processing, storage, and detoxification before it is distributed throughout the rest of the body.

The hepatic portal system allows the liver to regulate the composition of blood coming from the digestive organs before it enters general circulation. Thus, option D is correct.

Learn more about the hepatic portal, here:

https://brainly.com/question/12897264

#SPJ6

what is an amino acid

Answers

Amino acids are organic compounds that contain amino and carboxyl functional groups, along with a side chain specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, although other elements are found in the side chains of certain amino acids.
Amino acids are Organic compounds

A salt marsh community includes egrets, gulls, and marsh crabs living together in a salt marsh. What does one female egret represent?

Answers

Answer:

It is a tool for keeping population in equilibrium state.

Explanation:

One female egret represents that she is a tool for the continuity of its breed or species because female egret lays eggs and reared their offspring in order to maintain its generation in that salt marsh area. These female egrets are the essential part of this salt marsh ecosystem due to the ability to control the population of other organisms through feeding behaviour. These female egrets helps in maintaining the equilibrium of the ecosystem by constantly producing baby egrets.

Other Questions
What is the answer??? what is the definition of mutual flux? What is the relationship between retar ding potential and the kinetic energy of the emitted photoelectrons? Which expression is equivalent to 2.8n 8.4? Nancy's mother has a large collection of shoes. The table shows how many pairs of shoes shehas in each style.flats9sandals 6pumps 10If Nancy's mother chose a pair of shoes at random, what is the probability that the shoeswould be pumps?Write your answer as a fraction or whole number.P(pumps) = helppppppppppppppppppppppp What is the total amount on an investment account of $450 at anannual simple interest rate of 3% at the end of 48 months? 2. What is the smallest country by land area on Earth? Find the area of the bigger triangle (will give brainlest) How many planets are there in our System? List them all. Executives for a grocery store chain want to survey their employees about a new policy regarding break times. Toget a representative sample of 40 employees, they randomly select 10 people from each of the different job titles atthe store (cashier, bagger, stocker, and manager). Which sampling method was used?O cluster samplingO simple random samplingO stratified random samplingO systematic random sampling Which of the following situations show that the object is accelerating? I. A truck changing directions II. A car speeding up in a high way III. A moving motorcycle comes to stop IV. A rolling ball moving with a uniform speed.a. I and IIb. I and IIIc. I, II, and IIId. I, II, III and IV Order the numbers from least to greatest.3.4, 31/5, 35.8%, 3.64, 322% Snug Harbor Realty Co., had a checking account in 1st National Bank. When construction work was obtained by Snug Harbor, its superintendent, Magee, would examine the bills submitted for labor and materials. He would instruct the bookkeeper which bills were approved, and the bookkeeper then prepared the checks in accordance with his instructions. After the checks were signed by the proper official of Snug Harbor, Magee picked them up for delivery. Instead of delivering certain checks, he forged the signatures of the respective payees as endorsers and cashed the checks. The drawee bank then debited the Snug Harbor account with the amount of the checks. Snug Harbor claimed this was improper and sued the bank for the amount of the checks. The bank claimed it was protected by the impostor rule. Required:Will the bank be successful? what is M and N? THE PICTURE IS DOWN You have found two job offers for the beginning of the summer. Each job is only 10 days long. Job Offer #1: The offer is to receive $0.25 for accepting the job. After day one you have made a total of $1, $4 total after two days of work, $16 total after three days, etc. Job Offer #2: The offer is $16 per day with no acceptance bonus. Write an equation to represent Job Offer #1 and Write an equation to represent Job Offer #2 que es la responsabilidad digital ? Under the Massachusetts Bay Colony's theocratic system, which of the following was true? A. Only Puritans could own businesses. B. Puritans were exempt from paying taxes. C. Only Puritans could vote for governor. D. Puritans were exempt from certain laws. Using the mRNA Codon Chart #1, the codon GCA codes for the amino acid ...? A computer programmer, a dentist, and a teacher are all examples of what, because they perform a job or service?A - human resource B - natural resourceC - capital goodsD - quotas