Probes that bind under very stringent hybridization conditions resulting in the differential binding over one nucleotide are called ________. Probes that bind under very stringent hybridization conditions resulting in the differential binding over one nucleotide are called ________. short, variable repeats VNTRs microsatellites allele-specific oligonucleotides (ASOs) generation-specific probes

Answers

Answer 1

Answer:

allele-specific oligonucleotides (ASOs)

Explanation:

An allele-specific oligonucleotide (ASO) is a piece of synthetic DNA that is short and usually a complementary to the sequence of a target DNA, It is an oligonucleotide of 15–21 nucleotide bases in length.

It acts as a probe in southern blot assay or simpler Dot blot assay to identify the presence of a target.

It is an important research tool in genetic testing, forensics, and Molecular Biology.

The probes can detect a difference of as little as 1 base in the sequence targeted.


Related Questions

Which kingdom is completely composed of you unicellular organisms that are prokaryotic

Answers

Eubacteria is the kingdom that is composed of unicellular organisms that are prokaryotic.


Is a sandstone or a limestone older

Answers

Answer:

I think that limestone is older

Explanation:

A lysosome is the organelle inside of a cell that is responsible
for digestion. The lysosome performs the same basic function
as which system in animals?
Digestive System
Circulatory System
Nervous System
Respiratory System

Answers

Digestive System
Hope this helps!

did you know that 83,915,501,421 usd are spent on illegal drugs?/!?!?

Answers

Answer:

yes iknow that do you know?

Answer:

omg I never knew that

Explanation:

wht is it

Bare rock
Colonised by
pioneer species
(e.g. moss)
Decomposition
creates a layer
of topsoil
Grasses grow
and displace the
pioneer species
More nutrients
in soil allows for
shrub growth
Increase in soil
depth allows for
growth of trees
What ecological process is shown above?
O migration
O primary succession
O secondary succession
O nitrification

Answers

Answer:

Primary Succession

Explanation:

Primary Succession is a barren area exposed by a retreating glacier

Which of the following is a characteristic of many eukaryotes?

a. Many eukaryotes have no DNA
b. Many eukaryotes have many cells
c. Many eukaryotes have no cell membrane
d. Many eukaryotes have DNA in cytoplasm

Answers

Answer:

c

Explanation:

The statement that represents a characteristic of many eukaryotes is that many eukaryotes have many cells. Thus, the correct option for this question is B.

What do you mean by Eukaryotes?

Eukaryotes may be defined as the type of living organisms that significantly have a well-defined nucleus along with other membrane-bound cell organelles. These organisms have a complex level of compartmentalization.

According to the context of this question, almost all eukaryotes have DNA in their nucleus. This is because it is considered its genetic material. So, options A and D are eliminated.

The cell membrane is the characteristic feature of every eukaryote because this membrane separates the inner environment of the cell from external conditions. So, option C is eliminated as well.

Therefore, the statement that represents a characteristic of many eukaryotes is that many eukaryotes have many cells. Thus, the correct option for this question is B.

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Which metamorphic rock is foliated and coarse grained?
a. quartzite
b. hornfels
C. slate
d. gneiss

Answers

Answer:

Gneiss

Explanation:

Quartzite and Hornfels are both non-foliated metamorphic rocks. Slate is foliated but is fine-grained, which leaves us with Gneiss, which is a foliated, coarse-grained metamorphic rock

The process of. Produces a new copy of an organisms genetic information which is passed on to a new cell

Answers

what process produces new copy of organisms genetic information?

DNA replication

What is the function of digestive enzymes ?
Answer in brief..I'll reward.​

Answers

➜ Digetive enzymes convert the large and insoluble food molecules into small water molecules in the process of digestion. For example, pancreas secretes enzymes like amylase, trypsin and lipase. The enzyme amylase breakdown the starch, trypsin breaks down the proteins and lipase breaks down emulsified fats into simple sugars, amino acids and fatty acids, respectively.

How do elephants warn their family groups of a dangerous predator in their habitat?

A. They use their trunks to make loud sounds.
B. They stomp their feet to create noise.
C. They use their tusks to kick up dirt.
D. They flap their ears rapidly.

Answers

Answer:

How do elephants warn each other?

When they're excited, scared or happy, elephants make the trumpeting sounds you're probably familiar with. They vocalize in other, more complicated ways, too — low-pitched rumbles outside our range of hearing. At less than 20 hertz, these infrasonic calls are often used to alert each other to danger.

Explanation:

soo A

What are biodiversity hot spots?
O A. Areas where large numbers of earthquakes and volcanic eruptions
occur
B. The warmest and driest ecosystems on Earth
C. Areas where the average annual temperature exceeds 40°C
O D. The richest and most threatened reservoirs of life on Earth

Answers

Answer: D  

Explanation: Yet biodiversity hotspots are, by definition, in a conservation crisis. To be classified as a biodiversity hotspot, a region must have lost at least 70 percent of its original natural vegetation, usually due to human activity. There are over 30 recognized biodiversity hotspots in the world


Please help
1) What process determines the
potential of species to
increase in numbers?

Answers

answer:.

reproduction

Summarize the theory of Continental Drift in your own words.

Answers

E? Mate where is the picture??...

Which three organ systems work together to take in oxygen and other
nutrients and deliver them to an animal's body cells?
A. Respiratory, because it takes in gases from the external
environment
B. Circulatory, because blood absorbs nutrients and carries them to
other cells
c. Nervous, because it controls the body functions and responses to
stimuli
D. Digestive, because it breaks down the nutrients in food into
substances that can be used by cells

Answers

Answer:A, B and D

Explanation:Respiratory takes in oxygen and digestive absorbs nutrients from the food while the circulatory deliver those through the entire body.

A B D im on the exam

Caenorhabditis elegans is a species of soil-dwelling nematode (roundworm) that feeds on soil bacteria, including Bacillus thuringiensis.

a. True
b. False

Answers

Answer:

b. False

Explanation:

Caenorhabditis elegans is a well-known species which is widely used in genetic experiments (i.e., it is a model organism). C. elegans is a soil nematode that eats bacteria, but its survival depends on the ability to distinguish toxic from non-toxic ones. C. elegans cannot feed Bacillus thuringiensis because this bacterium produces a toxin Bt protein that damages its gut, thereby producing the death of this nematode. Interestingly, it has been proposed that Bt toxin pathways might be exploited for the design of new generation nematicides.

What is the BEST explanation that accounts for the climate in tropical areas near the equator?
A) When the air is cooled it can absorb more moisture, and it tends to rise. Thus air near the poles, heated by the direct rays of the sun, absorbs moisture and rises.
B) When the air is warmed by the sun it can absorb more moisture, and it tends to rise. Thus air near the poles, heated by the direct rays of the sun, absorbs moisture and sinks.
C) When the air is cooled it can not absorb as much moisture, and it tends to sink. Thus air near the equator, heated by the indirect rays of the sun, absorbs no moisture and sinks.
D) When the air is warmer by the sun it can absorb more moisture, and it tends to rise. Thus air near the equator, heated by the direct rays of the sun, absorbs moisture and rises. Higher in the atmosphere the air cools again, forming clouds that produce rainfall.

Answers

Answer:

D

Explanation:

The equator is hit with direct sunlight, and is known to be very humid and have lots of rainfall.

My reasoning is in this picture below

In real life, sequencing data is never as clean and clear as the sequencing data presented here. Typically, sequencing machines do not read eDNA sequences perfectly, occasionally mistaking a C for a T, for instance (though this is often <1% of the time). Additionally, there will even be some variability of the barcode among individuals of the same species! How can sequences without 100% identity to a reference still be useful for biodiversity assessment

Answers

Answer:

by using consensus sequences and complementing DNA barcoding data with other sources of information (e.g., morphological, and ecological data)  

Explanation:

DNA barcoding refers to the taxonomic method used to identify and classify species by comparing DNA sequences. Some of the most used barcoding genes include the 16 subunit (16S) of the ribosomal RNA (rRNA) gene in prokaryotes, the Cytochrome c Oxidase I (COI) gene in animals, the Internal Transcribed Spacer (ITS) sequence in fungi and the RuBisCO gene in plants. A good gene for DNA barcoding purposes must exhibit intraspecific variability, conserved regions in order to synthesize appropriate PCR primers, and the sequence need to be short in length (100 to 1000 bases). The main problem associated with this identification method is the confidence value between genetic sequences. For example, when COI sequences are identical, there is a 6 percent of chance that these sequences belong to different species. On the other hand, the percentage of intraspecific variability among DNA barcoding sequences is variable, even among closely related species. This problem, as well as bioinformatic errors related to sequence reads, can be partially overcome by aligning genetic sequences (including outgroups) in order to obtain consensus sequences. Moreover, taxonomists reasonably argue that DNA barcoding data should be complemented with morphological and ecological data in order to achieve accurate species identification.

Antibiotic resistant bacteria are becoming more and more prevalent, leaving us in desperate need of novel antimicrobial compounds to replace the drugs that are now obsolete in our hospitals and medical clinics. A high throughput screen of 700,000 compound library has allowed you to identify one molecule capable of killing extensively drug resistant tuberculosis at concentrations 1000X lower than currently used therapeutics. The only problem is, you don't know the molecular target. Develop an experimental strategy to identify the molecular target of your new antibiotic. Make sure to use some of the techniques described in the chapters covered throughout Units 1-6, and be creative. There isn't one correct answer!'

Answers

Answer:

Explanation:

We know the molecules equipped for killing broadly drug-resistant tuberculosis. This requires that we build up an experimental test system to distinguish the molecular target of your new antibiotic.

These steps include:

Gather proteomics data of tuberculosis and gather structural data of all proteins inside it.  

At that point perform molecular docking with the molecule you as of now have.  

This can yield the molecular target for the new antibiotic.

How much energy will the cheetah get if it eats the gazelle?

Answers

Answer:

alot

Explanation:

The air in the thermosphere becomes thinner and thinner until it fades into space. True or false

Answers

Answer:

Well to be honest there are no unmistakable separating lines. Each layer mixes into the layer above it and the layer underneath it. Researchers are certain that the air becomes thinnerand more slender farther from the Earth's surface, until it at long last blurs off into space.

Explanation:

Answer:true

i cant explain they wont let me put in l-i-n-k-s

Using the picture below, what is the BEST question that a student could ask that would help determine which aquatic biome this is depicting?

A.
Does the temperature of the water affect the salinity?

B.
Is there a variation in water pressure throughout the day?

C.
Is there a fluctuation in water salinity throughout the day?

D.
Is there variation in water temperature throughout the day?

Answers

Answer:

Explanation:

Using the picture below, what is the BEST question that a student could ask that would help determine which aquatic biome this is depicting?

A. x

Does the temperature of the water affect the salinity?

B. x

Is there a variation in water pressure throughout the day?

C. x

Is there a fluctuation in water salinity throughout the day?

D. right

Is there variation in water temperature throughout the day?

how to cultivate yam​

Answers

Explanation:

Yams are produced through slips, which have been generated again from sprouts of mature yams, rather than seeds, as most other crops are. Split a yam in part and place one half in a bowl of cool water to develop sprouts.

the angle of reflection is the angle between the normal line and the

Answers

Answer:

Reflected ray

Explanation:

The angle between a reflected ray and the normal drawn at the point of incidence to a reflecting surface is the angle of reflection.

D
Question 2
1 pts
Because the ANS is a visceral motor system, afferent pathways are of no importance
and actually are rarely found.
True
False​

Answers

Answer:

The correct answer is - false.

Explanation:

The ANS is a system consider as the visceral motor system, however, the afferent pathways are the system that is used to flow the signals to the control center from taking from the receptor and are very essential for the homeostasis communication of the nervous system.

This pathway involves the neurons with the signals to the spinal cord and the brain as sensory data. This pathway is very common to provide communication between the receptor and spinal cord.

Which of the following does NOT describe vacuoles?

both plants and animals have vacuoles

they are the largest organelle in plant cells

they store water in plants and help give them structure

they are the place where cell respiration takes place

Answers

Answer:

they are the place where cell respiration takes place

Explanation:

Cellular respiration is a series of metabolic reactions used by the cell to create energy (ATP), these reactions occur partially in the cytoplasm, but mostly in the mitochondria. On the other hand, vacuoles store water in plants and help give them structure, are the largest organelle in plant cells, and both plants and animals have them. Therefore the answer is, they are the place where cell respiration takes place.

HELP ME PLEASE SOMEONE :(

Answers

Answer:

D

Explanation:

The other person is right, pls give them brainliest pls?

If a plant fall which way will the roots grow

Answers

in the direction of the gravitational pull

Answer: The roots grow whichever way gravity will push them.


Give two reasons of the modification of stem of a plant in different forms,

Answers

1.underground stem -- for storage of food ex. potato , ginger.

2.spines for protection ex. citrus , bougainvillea

Matching type:
1. ribosome
2. smooth ER
3. mitochondrion
4. nucleus
5. Golgi apparatus
6. lysosome
7. centriole
8. cytoskeleton
9. inclusion
10. plasma membrane
11. nucleolus
a. main site of ATP synthesis
b. encloses the chromatin
c. sac of digestive enzymes
d. examples include glycogen granules and ingested foreign materials
e. forms basal bodies and helps direct mitotic spindle formation
f. site of protein synthesis
g. forms the external boundary of the cell
h. site of lipid synthesis
i. packaging site for ribosomes
j. packages proteins for transportation
k. internal cellular network of rodlike structures

Answers

Answer:

1. ribosome---- f. site of protein synthesis

2. smooth ER---- h. site of lipid synthesis

3. mitochondrion---- a. main site of ATP synthesis

4. nucleus---- b. encloses the chromatin

5. Golgi apparatus---- e. forms basal bodies and helps direct mitotic spindle formation and j. packages proteins for transportation

6. lysosome---- c. sac of digestive enzymes

7. centriole---- k. internal cellular network of rod like structures  

9. inclusion---- d. examples include glycogen granules and ingested foreign materials

10. plasma membrane---- g. forms the external boundary of the cell

11. nucleolus---- i. packaging site for ribosomes

Explanation:

Ribosome is considered as a site of protein synthesis,  smooth endoplasmic reticulum is the site of lipid synthesis , mitochondria is the main site of ATP production, nucleus encloses the chromatin material inside it,  Golgi apparatus forms basal bodies and helps direct mitotic spindle formation and also packages proteins for transportation,  lysosome is a sac of digestive enzymes , centriole consists of internal cellular network of rod like structures  , inclusion has examples include glycogen granules and ingested foreign materials,  plasma membrane forms the external boundary of the cell  and nucleolus has the function of packaging site for ribosomes.

ribosome - f. site of protein synthesissmooth ER - h. site of lipid synthesismitochondrion - a. main site of ATP synthesisnucleus - b. encloses the chromatinGolgi apparatus - j. packages proteins for transportationlysosome - c. sac of digestive enzymescentriole - e. forms basal bodies and helps direct mitotic spindle formationcytoskeleton - k. internal cellular network of rodlike structuresinclusion - d. examples include glycogen granules and ingested foreign materialsplasma membrane - g. forms the external boundary of the cellnucleolus - i. packaging site for ribosomes

The functions of various cell organelles are as follows: Ribosome is responsible for protein synthesis, smooth endoplasmic reticulum synthesises lipids, mitochondria produces ATP, nucleus encloses chromatin material, Golgi apparatus forms basal bodies and aids in mitotic spindle formation and protein packaging for transportatio.

Lysosome contains digestive enzymes, centriole is composed of a cellular network of rod-like structures, inclusion contains glycogen granules and foreign materials, pla

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what are the limitations of cell theory​

Answers

Answer:

The shortcomings or drawbacks of cell theory are: Viruses are considered as acellular entities or organisms that do not have cell machinery, yet they are taken into account as organisms in this cell theory. Matthias Schleiden and Theodor Schwann did not know the mechanism of the cell.

Explanation:

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