The isoelectric point; pI, of the protein trypsin is 10.5 while that of uricase is 633 What is the net charge of trypsin at pII 5.1 What is the net charge of uricase at pII 5.7 The isoelectric point of alanine is 6.01 isoleucine 6.02 During paper electrophoresis at pH433 toward which electrode does alanine migrate? During paper electrophoresis at pHI 7.1 toward which electrode does isoleucine migrate?

Answers

Answer 1

At pII 5.1, the net charges of trypsin is positive.

At pII 5.7, the net charges of uricase is negative.

What are the net charges of trypsin and uricase at pII?

Proteins can carry positive or negative charges depending on the pH of their environment. The isoelectric point (pI) is the pH at which a protein has a net charge of zero.

If the pH is below the pI, the protein carries a net positive charge, and if the pH is above the pI, it carries a net negative charge.

In the given question, trypsin has a pI of 10.5, and at a lower pH of pII 5.1, it will have a net positive charge. This means that trypsin will migrate towards the cathode (negative electrode) during paper electrophoresis at pII 5.1.

On the other hand, uricase has a pI of 6.33, and at a slightly higher pH of pII 5.7, it will have a net negative charge. Therefore, uricase will migrate towards the anode (positive electrode) during paper electrophoresis at pII 5.7.

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Related Questions

Balance the equation:

Answers

Answer:

3SiO2  +  2CaC2 ------>   3Si  +   2CaO  +  2CO2

Explanation:

PLEASE HELPPP ILL GIVE BRAINLIEST!!!!!!!!!!!!!

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veins is the correct answer

Answer:

it travels through the veins

Help me with this please

Answers

= 0.2+560.66JK-2eF

use this app : Photomath

It will help you :)

What temperature scale is used in the ideal gas equation?

A. Kelvin
B. Fahrenheit
C. Celcius
D. Doesn't matter​

Answers

kelvin

hope that helps^^

A. Kelvin would be the correct answer

Ha hatdog hatdog hatdog because?​

Answers

Answer:

what's this? s*it fellow

uska dil ker raha tha isleye

-
In the reaction: 2X2Y3 + 3Z(QR)4 4X(QR)3 + 3ZY2
What is the charge of the "X" cation needed to form these compounds?

Answers

Answer:

That's simple it's the caca of 54 32 x (5) b ÷ 5683jvl done so your answer is 0

consider a sample of compound contain 1.719c 0.284gh so find emprisical formula

Answers

So it should really scientifically speaking here be 420.566c

Water's ________ specific heat makes it heat up and cool down slowly; this stability has helped water support life.

A) high
B) low
C) absence of

Answers

Answer:

high

Explanation:

Answer:(A)

Explanation:

37.5g of nitrogen reacts with 15.5 g of hydrogen. What mass of ammonia can be made? What is the limiting reactant?

Answers

Answer:

Limiting reactants or limiting reagents decide the amount of product formed and the amount of excess reagent used.

(HELP) how can you make the metal chair less cold when you sit on it?

Answers

Answer:

metal can hold heat keep it near fire

i need help with chemistry asap. the copounds Ch3Oh and Ch3CooH react in the presence of an acid catalyst to form. A. a carboxylic B. an aldehyde C. a ketone and D. an ester.​

Answers

The answer is B because

why are gases able to spread out and fill whatever container they are in

Answers

Answer:

gases are able to spread out and fill whatever container they are in because they do not have. a definite shape their molecules are not tightly packed so they can cover over a vast area like our atmosphere.

HELP ASAP!!!!!! 30 PTS
Mouth, throat, kidney, and stomach cancers can each be linked to a common cause.

Which can be a cause of any of these cancers?

UV radiation
tobacco use
diabetes
sickle cell anemia

Answers

Answer:

Tobacco use

Explanation:

Smoking causes cancer, heart disease, stroke, lung diseases, diabetes, and chronic obstructive pulmonary disease (COPD), which includes emphysema and chronic bronchitis. Smoking also increases risk for tuberculosis, certain eye diseases, and problems of the immune system, including rheumatoid arthritis

Answer:

c. Tabaco use

Explanation:

What is the Ka of a 0.479 M
solution of acetic acid
(CH3COOH) with a pH of 4.76?
r

Answers

Answer:

6.31x10^-10

Explanation:

Answer: 6.31 x 10^-10

Explanation:

What are mineral acids?

Answers

Answer:

mineral acids are obtained from one or more inorganic compounds which is a compound that lacks carbon and hydrogen bonds. Inorganic compound examples include water and carbon dioxide. Something all mineral acids do is form hydrogen ions and the joined together bases when it is dissolved  in water.

Explanation:

Please help!!

You have 5 moles of marbles. How many marbles is this?

Answers

Answer:

The answer is 3.011x^24 marbles

Explanation:

6.022 x 10^23 x 5 = 3.011 x 10^24

plz someone help with this

Answers

Answer:

4 I believe is false and 5 is D I think

Answer:

4.) A. (TRUE)

5.) B. (THE WEATHER AND CLIMATE)

Explanation:

I HOPE IT HELPS YOU;)

How many pounds of ice are required to absorb 4900 kJ of heat as the ice melts? The heat of fusion of water is 0.334 kJ/g.

Answers

Answer:

m = 32.34 pounds of ice.

Explanation:

In this case we need to use the following expression of heat:

q = m * ΔHf   (1)

Where:

q: heat absorbed in J or kJ

m: mass of the compound in g

ΔHf: heat of fusion of the water in kJ/g

We are asked to look for the mass of ice in pounds, so after we get the grams, we need to convert the grams to pounds, using the following conversion:

1 pound --------> 453.59 g   (2)

So, we have the heat and heat of fusion, from (1) let's solve for the mass, and then, using (2) the conversion to pounds:

q = m * ΔHf  

m =  q / ΔHf

m = 4900 / 0.334 = 14,670.66 g of ice

Now, the conversion to pounds:

m = 14,670.66 g * 1 pound/453.59 g

m = 32.34 pounds of ice.

Hope this helps  

Why does the moon's gravitational pull have more effect on Earth's tides than the sun's gravitational pull?


A. The moon is closer to the Earth than the sun.

B.The moon has more mass than the sun.

C. The moon is more dense than the sun.

D. The moon has a greater diameter than the sun.

Answers

I believe it’s c because if you really read and close read it will make more sense
It’s A. The moon is closer to the earth than the sun

[please answer fast!] If the hydroxide ion concentration is 2x10^-3 M, what is the pH of the solution? Show your working.

Answers

Answer:

pH = 11.30

Explanation:

pH is defined as -log [H⁺]

To find [H⁺] from [OH⁻] -Concentration of hydroxide ion-, we must use the equilibrium of water:

H₂O(l) ⇄ H⁺(aq) + OH⁻(aq)

Keq = 1x10⁻¹⁴ = [H⁺] [OH⁻]

[H⁺] = 1x10⁻¹⁴ / [OH⁻]

[H⁺] = 1x10⁻¹⁴ / 2x10⁻³M

[H⁺] = 5x10⁻¹²M

pH = -log (5x10⁻¹²M)

pH = 11.30

how does the total mass of each object increases the amount of force that is needed to get them moving at 5 m/s increase by about 200N demonstrate newtons 2nd law

Answers

Answer:

The answer is below

Explanation:

Newton's second law of motion states that the force applied to an object is directly proportional to the rate of change of momentum with respect to time, going in the same direction as the force.

Let F = force, m = mass of object, v = velocity of object, mv = momentum.

F = d/dt(mv) = m(dv / dt) = ma; a = acceleration.

Let us assume that the object starts from rest to 5 m/s within 1 seconds, hence:

F = m(dv / dt)

200 N = m[(5 m/s - 0 m/s) / (1 s)]

200 = 5m

m = 40 kg

How many grams are in 21.4 mol Cl2 gas?

Answers

Cl2 = 70.9 g/mol

=

[tex]21.4 \: mol \times \frac{70.9 \: g}{1 \: mol} = 1517.26 \: g[/tex]

.........m.......................






i need ur help guys​

Answers

Answer:

Uses of Sodium chloride:

1.it is used as table salt in our diets

2. it is used as a food preservative

3. It is used as an industrial source of sodium and chlorine

Uses of Sodium hydroxide:

1. It is in the laboratory as an alkali in the neutralization of acids

2. It is used in the manufacture of soap

3. It is used in the refining of petroleum

Uses of Sodium carbonate:

1. It is used in the manufacture of detergents and glass

2. It is used to soften hard water

3. it is used to standardize acids in the laboratory

Uses of Sodium sulphate

1. It is used in the manufacture of detergents and glass

2. It is used as a purgative

3. It is used to treat wood-pulp for paper making.

Uses of Potassium nitrate

1. It is used in making gunpowder

2. It is used as rocket propellant

3. It is used in making fertilizer

Uses of Potassium chlorate

1. It is used as an oxidizer

2. It is used in making explosives

3. it is used as a disinfectant

Uses of Potassium permanganate (vii)

1. It is used as an oxidizing agent

2. It is used as a disinfectant

3. It is used as an analytical reagent

Uses of Potassium chloride

1. It is used for the manufacture of fertilizer

2. It is used as an alternative salt to sodium chloride

3. It is used in medicine to treat low blood pressure

2. Potassium nitrate and potassium chloride are used in the manufacture of fertilizers.

Potassium nitrate is used in fertilizers because it provides two important plant nutrients, nitrogen and potassium to plants.

Potassium chloride (potash)  is used in fertilizers because it efficiently deliver the important plant nutrient, potassium, to plants.

Explanation:

Uses of Sodium chloride:

1. it is used as table salt in our diets

2. it is used as a food preservative

3. It is used as an industrial source of sodium and chlorine

Uses of Sodium hydroxide:

1. It is in the laboratory as an alkali in the neutralization of acids

2. It is used in the manufacture of soap

3. It is used in the refining of petroleum

Uses of Sodium carbonate:

1. It is used in the manufacture of detergents and glass

2. It is used to soften hard water

3. it is used to standardize acids in the laboratory

Uses of Sodium sulphate

1. It is used in the manufacture of detergents and glass

2. It is used as a purgative

3. It is used to treat wood-pulp for paper making.

Uses of Potassium nitrate

1. It is used in making gunpowder

2. It is used as rocket propellant

3. It is used in making fertilizer

Uses of Potassium chlorate

1. It is used as an oxidizer

2. It is used in making explosives

3. it is used as a disinfectant

Uses of Potassium permanganate (vii)

1. It is used as an oxidizing agent

2. It is used as a disinfectant

3. It is used as an analytical reagent

Uses of Potassium chloride

1. It is used for the manufacture of fertilizer

2. It is used as an alternative salt to sodium chloride

3. It is used in medicine to treat low blood pressure

2. Potassium nitrate and potassium chloride are used in the manufacture of fertilizers.

Potassium nitrate is used in fertilizers because it provides two important plant nutrients, nitrogen and potassium to plants.

Potassium chloride (potash)  is used in fertilizers because it efficiently deliver the important plant nutrient, potassium, to plants.

The
tell
you which element it is.
Atoms of the same element with a different number of NEUTRONS are
Atoms of the same element with a different number of ELECTRONS are
The PROTONS and NEUTRONS Can/Can't leave an atom during a
chemical reaction. The Electrons Can/Can't leave an atom during a
chemical reaction.
In a balanced atom, the
equal the

Answers

Answer:

Isotopes

Explanation:

Isotopes are atoms of the same element that contain an identical number of protons, but a different number of neutrons. Despite having different numbers of neutrons, isotopes of the same element have very similar physical properties.

Nitrogen gas is being withdrawn at the rate of 4.5 g/s from a 0.15-m3 cylinder, initially containing the gas at a pressure of 10 bar and 320 K. the cylinder does not conduct heat, nor does its temperature change during the emptying process. What will be the temperature and pressure of the gas in the cylinder after 5 minutes? What will be the rate of change of the gas temperature at this time? Nitrogen can be considered to be an ideal gas with Cp*=30 J/mol.K.

Answers

Answer:

Final temperature = 152.57K,

Pressure = 0.6907 bar.

dT/dt = - 1,151 K/s.

Explanation:

The first thing to do here is to write out the equation for mass balance as given below:

dN/dt = N -------------------------------------------------------------------------------------------(1).

N = P/T, then, substitute the values given in the question into:

d[p/T]/ dt = [- 4.5/28 × 8.314]/0.15 = - 8.9 × 10⁻⁵ bar/K.s.

Thus, there is the need to integrate, Integrate [p/T]f = 10/320 - 8.9 × 10⁻⁵ bar/K.s. ------------------------------------(2).

NB; fT = final temperature, fP = final pressure and iT = initial temperature.

Also, [ fT]³⁰/₈.₃₁₄/ [fP] = [iT]³⁰/₈.₃₁₄/ Pi] = [ 320]³⁰/₈.₃₁₄/ 10.

Therefore, [fT]³⁰/₈.₃₁₄ = 109.52 × 10⁶.

Final temperature=  [fP]³⁰/₈.₃₁₄ × 169.05.

Note that fP/ [fP]³⁰/₈.₃₁₄ × 169.05 = 10/320 - 8.9 × 10⁻⁵.

Therefore, [fP]¹ ⁻ ³⁰/₈.₃₁₄ = 0.7651.

Hence, Final temperature = 152.57K,

Pressure = 0.6907 bar

dT/ dt = N[RT]² / Cv . PV.

R = 30 - 8.314 = 21.86 J/mol K.

Then, the rate of change of the gas temperature at this time = dT/dt = - 1,151 K/s.

What is the volume, in liters, of a 0.2 M solution containing 8.5 grams of AgNO3?

Answers

Answer:

0.25L or 250Ml

Explanation:

I am not that good but as far as my calculations this must be the answer

What are the characteristics of a metallic bond? PLEASE HELP

Answers

Answer: Metallic bonding is a type of chemical bonding and is responsible for several characteristic properties of metals such as their shiny lustre, their malleability, and their conductivities for heat and electricity. Both metallic and covalent bonding can be observed in some metal samples

PLEASE MARK ME BRAINLIST

Electrons move freely among positively charged ions. This is the characteristics of a metallic bond. Therefore, the correct option is option B.

Valence electrons in a metallic link are not bound to a specific pair of atoms, but rather travel freely among a "sea" of positively charged metal ions. These electrons are not assigned to any specific atom and are shared by all metal ions in the metallic lattice.

The electron sea model depicts the valence electron arrangement as a "sea" of negatively charged electrons encircling positively charged metal ions. Metals have strong electrical and thermal conductivity due to electron mobility.

Therefore, the correct option is option B.

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When calculating the AH of a reaction, why is it important that you include the correct stale symbols in your balanced chemical equation?

Answers

Answer: c. so that you use the correct energy states

Explanation:

Enthalpy is the energy release or energy produced when a chemical reaction is carried out.

Enthalpy change is calculated by substracting the energy of reactants from the energy of products.

[tex]\Delta H=H_{products}-H_{reactants}[/tex]

As the energies are different for different states and thus the correct stale symbols in your balanced chemical equation must be given so that we use the correct energy values.

A sample of an oxide of nitrogen is found to contain 30.4% nitrogen. What is its empirical formula?

Answers

Answer:

If nitrogen = 30.4% then oxygen = 100-30.4 = 69.6%

divide each % value by atomic mass

N = 30.4/14 = 2.17

O = 69.6/16 = 4.35

Divide each y smaller:

N = 2.17/2.17 = 1

O = 4.35/2.17 = 2

Explanation:

"The empirical Formula will be [tex]NO_{2}[/tex]."

What is empirical formula?

In chemistry, an empirical formula is the simplest thought of the entire number quantity of atoms in a molecule.

It is given that N = 30.4%.

The % of O can be determined as: 100-30.4%= 69.65.

Now, after dividing % value with atomic mass, we get.

Mass of  N= 14 and Mass of O = 16.

N= 30.4/14= 2.17

O = 69.6/ 16= 4.35

It can be seen that N has less number as compared to O . So, divide each with 2.17.

N= 2.17/2.17 = 1

O = 4.35/2.17=2

So the empirical formula will be [tex]NO_{2}[/tex].

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write the ground state electronic configuration of an atom having atomic mass number 37 and number of neutrons 20.​

Answers

Answer: The ground state electronic configuration of an atom having atomic mass number 37 and number of neutrons 20 is [tex]1s^22s^22p^63s^23p^5[/tex]

Explanation:

Atomic number is equal to the number of protons.

Mass number is the sum of number of neutrons and number of protons.

Mass number =  number of neutrons + number of protons

37 = 20 + number of protons

number of protons = 17

The atomic number is specific to an element, and the element with atomic number 17 is chlorine.

Electronic configuration represents the total number of electrons that a neutral element contains.

[tex]Cl:17:1s^22s^22p^63s^23p^5[/tex]

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