What are the advantages and disadvantages of the serial dilution-agar plate procedure?​

Answers

Answer 1

Answer:

Advantages

- Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies.

- In biochemistry, serial dilution is used to obtain the desired concentration of reagents and chemicals from a higher concentration.

- In pharmaceutical laboratories, serial dilution is performed to receive the necessary concentration of chemicals and compounds as this method is more effective than individual dilutions.

- In homeopathy, homeopathic dilutions are used where a substance is diluted in distilled water or alcohol. It is believed than dilution increases the potency of the diluted substance by activating its vital energy.

Disadvantages

- An error might occur during the propagation of the sample, and the transfer inaccuracies lead to less accurate and less precise transfer. This results in the highest dilution to have the most inaccuracies and the least accuracy.

- Because serial dilution is performed in a stepwise manner, it requires a more extended period of time which limits the efficiency of the method.

- Serial dilution only allows the reduction of bacteria/cells but not the separation of bacteria/cells like in other techniques like flow cytometry.

- This technique also requires highly trained microbiologists and experts in aseptic techniques.


Related Questions

Chromosomes are...

A.DNA material coiled up in a pile
B.DNA material in pairs
C. DNA material in single strands
D. DNA material outside of the nuclear membrane
WILL GIVE BRAINLIEST

Answers

A. DNA material coiled up in a pile.

Glucose is a large molecule that is transported across the cell
membrane through a gated channel, a type of transport protein
used during what
diffusion

Answers

Facilitated diffusion

The type of transport protein used during glucose transportion across the cell membrane is facilitated diffusion.

Glucose is the main energetic substrate of the cell and requires a transporter protein in the cell membrane for its entry.

Proteins found on the surface of the membrane intervene in facilitated diffusion, which, when they make contact with the molecules, bind to them and allow their entry.

This route is used by molecules that are larger in size, with properties other than the membrane (polar), or are charged and do not cross the membrane freely; for example glucose.

The transport of glucose across the cell membrane is carried out by two families of membrane proteins:

Sodium-coupled glucose transporters are expressed mainly in epithelia that are responsible for the absorption and reabsorption of nutrients.Glucose transporter (GLUT) are expressed in all cells of the body and allow glucose to move from one compartment to another.

Therefore, we can conclude that glucose enters cells through facilitating transport systems, glucose transporter are expressed in all tissues of the organism and transport glucose through its concentration gradient, hence the name of facilitated diffusion.

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A seed contains
A) cotyledons
B) xylem
C) stoma
D) phloem

Answers

answer:

a) cotyledons

explanation

Name the enzyme diagrammed below.

Answers

this is a aminoacyl- TRNA synthetase

The climate changes on Earth tend to be self-amplified. For example, the Earth is
currently going through a warming trend. As the Earth's temperature rises, the ice caps
and sea ice melt, reflecting less solar radiation. This results in more absorption of the
Sun's heat, causing the Earth's temperature to rise more, and the ice to melt more. This is
an example of what type of system?

Answers

Answer:

negative feedback

Explanation:

This is an example of negative feedback system.

What do you mean by negative feeback?

Negative feedback occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances.

Negative feedback (or balancing feedback) occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances.

A negative feedback is a self-regulatory system in which it feeds back to the input a part of a system's output so as to reverse the direction of change of the output.

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3. Calculate the kinetic energy of a paper clip shot from a slingshot. The paper clip's mass is 0.01 kg, and its velocity is 10 m/s.
a) 0.1 J
b) 0.25 J
c) 0.5 J
d) 1 J

Answers

Answer:

C

Explanation:

0.01kg*(10m/s)^2/2=0.5

Please help me i really don’t know

Answers

Answer:

I think its d or option 4

Explanation:

Please mark me brainliest


Oxygen is a product of the electron transport chain.
True or False

Answers

Answer:

true

hope this helps you ☺️☺️

Electron transport chain can be defined as the series of organic molecules and proteins which are embedded in the inner membrane of mitochondria. These molecules are involved in the shutting of electrons from FADH2 and NADH to the [tex]O_{2}[/tex].

The molecular oxygen is the final electron acceptor in this electron transport chain that leads to the formation of water and ATP as the end products. This chain takes place in the mitochondria in both animal and plant cells.

Hence, the given statement is true.

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A new trait that was never in the gene-pool before is called a

A) Adaptation

B) Mutation

C) Genetic Variation

D) normal

Answers

Answer:

B) Mutation

Explanation:

Mutation is a mistake in the Gene's I doesnt mean it's a bad thing or a good thing. It could be both

the green colour pigment present in leaves chlorophyll true or false​

Answers

Answer:

True

Explanation:

Chlorophyll produces a green pigment, which is why leaves look green

Answer:

true the green stuff is chlorophyll

Explanation:

The primary colors of visible light are:
Answer: red, green and blue

Answers

Answer:

Correct / true

Explanation:

The hormone insulin is secreted by the pancreas. Which activity is MOST likely to
trigger an increase in the levels of insulin circulating in the blood?
O A. driving
B. eating
O C. jogging
OD. sleeping

Answers

Answer:

Eating

Hope this helps! <3

help me plzzzzzzzzzzz ​

Answers

Answer:

box 4, because it has more atoms in the same amount of space

In a process called transpiration, plants get rid of excess water through pores in the leaves called stomata. This excess water is then released into the atmosphere as part of the water cycle. Which of the following terms best describes how the released water enters the atmosphere?
Group of answer choices

precipitation

condensation

capillary action

evaporation

Answers

Answer:

Evaporation

Explanation:

how does biodiversity help with farming

Answers

Answer:

biodiversity is the basis of agriculture and our food systems. agricultural biodiversity also performs ecosystem services such as soil and water conservation, maintenance of soil fertility, conservation of biota and pollination of plants, all of which are essential for food production and for human survival.

good luck :)

hopefully, this helps

have a great day !!

______refers to the ability to cause change or do work.
A.friction
B.energy
C.gravity
D.distance

Answers

Answer:

B

Explanation:

Ive studied rhis before

What is an amniotic egg?

Answers

Answer: The amniotic egg was an evolutionary invention that allowed the first reptiles to colonize dry land more than 300 million years ago. Fishes and amphibians must lay their eggs in water and therefore cannot live far from water. ... Within the chorion is the amnion, the membrane for which the amniotic egg is named.

Explanation:

The ADG1(ADP glucose pyrophosphorylase 1) is involved in flowering timing in plants. You are interested in developing a DNA probe using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu 1. determine the possible antisense oligo nucleotides possible from this amino acid sequence 2. How many total unique different oligonucleotide would need to be produced to cover all possibilities

Answers

Answer:

1)  UUC-AUG-AUC-AAG-UAA.

2) 48

Explanation:

1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence

Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.

we will use a codon table to show the codons and Anticodons for each of the Amino acids  

Amino Acid   Codons                                                         Anticodons

 Glu                GAA, GAG                                                    UUC, CUC

 His                 CAU, CAC                                                    AUG, GUG

 Asp         GAU, GAC                                                    AUC, GUC

 Val                 GUU, GUC, GUA, GUG                             AAG, GAC, UAC,CAC

 Leu   UUA, UUG, CUU, CUC, CUA, CUG  | UAA, CAA, AAG, GAG,UAG,CAG

From the table above

The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.

2)  How many total unique different oligonucleotide would need to be produced to cover all possibilities

A desired peptide sequence requires 16 anticodons

Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48

The ADG1(ADP glucose pyrophosphorylase is involved in flowering timing in plants and a DNA probe is made by using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu.

1. To determine the possible antisense oligonucleotides possible from this amino acid sequence:

Since, for every amino acid, there is a specific codon and anticodons are present so according to the given information, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA.

2) The total number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48

since the amino acid sequence is Glu-Glu-His-Asp-Val-Leu has 16 anticodons and each anticodon have 3 nucleotides so the possible number of oligonucleotides are  :

16×3 = 48.

What are codon and anticodon?

A codon is a trinucleotide sequence of RNA that corresponds to a specific amino acid.

An anticodon is a trinucleotide sequence complementary to that of a corresponding codon in a messenger RNA sequence.

Thus, according to codon and anticodon, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA and a number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48.

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What is exine is made up of????


what's wrong in asking these types to Question.. it's based on studies​

Answers

Answer:

sporopollenin

While the exine is composed of sporopollenin, a complex and highly resistant biopolymer containing fatty acids, phenylpropanoids, phenolics and carotenoids, the intine is largely composed of pectin and cellulose.

Explanation:

What is the transfer of
heat from one object to
another through direct
contact?

Answers

Answer:

condiction

Explanation:

conduction is the transfer of heat between substances that are in direct contact with each other.

Light moves differently through different materials. What are some factors that could change the amount of sunlight absorbed by Earth’s surface and atmosphere?

Answers

if clouds are covering the sun and most of the sky, if rain is absorbing the ground and the amount of moisture in the atmosphere

Break question


what pets would you want




(p.s. could you please answer one of my questions)​

Answers

mmm I really want a dog! thank u for this!
i really want a cat and i might get one

Pls someone help its a very easy question

Answers

Answer:

you got it right

Explanation:

The stage in the mentrual cycle usually lasting from 3to five days when fertilization does not occur.
A.ovulation
B.stimulation
C.menustration
D.reproduction​

Answers

It should be A) ovulation

Hope this helps!
Good luck!

Polymerase Chain Reaction (PCR) technology is used for

a. DNA identification
b. DNA repair
c. DNA amplification
d. DNA cleavage​

Answers

Answer:

The correct answer is c.

Explanation:

Polymerase Chain Reaction (PCR) technology is an in vitro enzymatic method that allows the amplification of a specific DNA sequence, that is, it is the DNA amplification technique by means of the polymerase chain reaction (PCR) is a technique that consists of the in vitro amplification of a specific DNA fragment. To carry out the amplification experiment, it is necessary to know, at least partially, the sequence of the fragment to be amplified (a gene, a part of a gene, a non-coding region, ...). Basically, it is about replicating the same DNA fragment over and over again and, for this, we must do in vitro what cells do in vivo to replicate their DNA. Generally, PCR begins with the denaturation or separation of the DNA double helix by heating the sample to a temperature between 94 and 96 ° C to break the hydrogen bonds that joined them, in this way each chain remains as a template for the synthesis of a new complementary strand of DNA.

plz answer ASAP will get ten points ​

Answers

Answer:

the 2nd and 4th

Explanation:

they contain more than one molecule due to them being different elements

2 and 4 ones because the person above me said so lol

The first stage of the cell cycle during which the cell matures and prepares to divide and copies
its DNA; The stage the cell stays in the most?

Answers

The first stage and the stage the cell stays in most is Interphase

The cell multiplies and creates a copy of its DNA during interphase. In the mitotic (M) phase, the cell divides into two new cells by dividing its cytoplasm and DNA into two sets.

What is the main role of interphase stage of cell cycle?

A cell consumes the bulk of its time in what is known as interphase, where it creates, duplicates its chromosomes, and acquires available to split. The cell then moves interphase, goes direct mitosis, and completes dividing.

The cell multiplies and the nuclear DNA is duplicated during interphase. The mitotic phase immediately follows interphase.

Chromosomes that have been duplicated are separated and distributed across daughter nuclei during the mitotic phase. Usually, the cytoplasm is divided as well, producing two daughter cells.

Therefore, The first stage and the stage the cell stays in most is Interphase.

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PLZ HELP ME ASAP


Read the article above and answer the following questions


1. The cardiovascular system includes:

A. the heart

B. blood

C. blood vessels

D. all of the above


2. Which of the following is NOT carried through the body by blood?

A. valves

B. carbon dioxide

C. nutrients

D. oxygen


3. Which of the following carries blood out of the heart?

A. arteries

B. veins

C. blood vessels

D. tubes


4. Which of the following is NOT a part of blood?

A. red blood cells

B. white blood cells

C. gorta

D. plasma


5. What is the heart largely composed of?

A. plasma

B. platelets

C. muscle

D. both B and C

Answers

all of the above  cause the cardiovacular system help you move your blood

Answer:

1. all above

2. valves

3. arteries

4. gorta

5. muscle

Explanation:

What is active transport?

Answers

Answer:

the movement of ions or molecules across a cell membrane into a region of higher concentration, assisted by enzymes and requiring energy.

Explanation:

Explain what would happen if the leaf tissue is kept at high temperature during the process of cell isolation

Answers

Answer:

Wilting of leaves occur.

Explanation:

if the leaf tissue is kept at high temperature during the process of cell isolation the leaf will shrink because of loss of all water. Due to high, temperature the stomata will be open and water is removed in order to remove heat from the body. A time comes that all water that is present in the cell will be removed and as a result wilting of leaves occur.

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For the reaction, ?N2 + ?H2 ? NH3, What is the maximum amount of NH3 which could be formed from 12.26 mol of N2 and 2.11 mol of H2? Answer in units of mol. Swifty Hardware reported cost of goods sold as follows. 2022 2021 Beginning inventory $ 31,000 $ 21,500 Cost of goods purchased 203,500 153,000 Cost of goods available for sale 234,500 174,500 Less: Ending inventory 35,000 31,000 Cost of goods sold $199,500 $143,500 Swifty made two errors: 1. 2021 ending inventory was overstated by $3,600. 2. 2022 ending inventory was understated by $6,550. Compute the correct cost of goods sold for each year. 2022 2021 Cost of goods sold which inequality represents all values for x for which the quotient below is defined? square root of 8x squared divided by the square root of 2x Find the distance between the points (-3,14) and (4,-10) Data for January for Bondi Corporation and its two major business segments, North and South, appear below: Sales revenues, North $ 561,000 Variable expenses, North $ 325,500 Traceable fixed expenses, North $ 67,100 Sales revenues, South $ 433,200 Variable expenses, South $ 247,100 Traceable fixed expenses, South $ 56,000 In addition, common fixed expenses totaled $151,900 and were allocated as follows: $78,900 to the North business segment and $73,000 to the South business segment. A properly constructed segmented income statement in a contribution format would show that the segment margin of the North business segment is: If a sales person receives a commission of 12% of sales what is the sales person commission on a $250,000 sale what is the value of x?2367 what are 5 proper noun for Mrs. Morton? how quickly is cyclin destroyed? If two angle measurements are 53 and 70, what must the third angle measurement equal to form a triangle?Please help please? 6y - 3 - 2y = -3 (substitution) Need help please show work Help plzzzzzzzzzzzzzzzz How to wright a fully elaborate paragraph on the story out of the dust What is the total surface area?? Please help me Im confused what is the continent with the letters coeaian A body accelerates uniformly from rest at 2ms for 5 seconds . calculate its average velocity in this time. BCRound your answer to the nearest hundredth.656B? George deposited $2,000 in his bank account, which offers 5 percent compound interestcalculated annually. What would be the principal amount available in George's account atthe end of two years?There would be a principal amount of $of 2 years.in George's account at the endO 2.1000 2.00002.005O 2.205 Which statement best completes the diagram? Culture of eastern and central Africa. Widespread poverty is a major problem many different languages are spoken. A. Most people practice traditional religions B.nearly all people live in large urban areas C. Many people descended from the Bantu D. The cuisine is mostly meat with few grains