What is the function of QuickLyse spin columns?

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Answer 1

Spin column- grounded nucleic acid sanctification is a solid phase birth system to snappily purify nucleic acids.

Spin Column- Grounded is one of the RNA birth styles that comprise of four stages videlicet lysis of cells, list of nucleic acid to silica gel membrane, washing the nucleic acid bound to the silica gel membrane, and elution of the nucleic acid. a small ‐ scale chromatography procedure in which fluid is moved through the column by centrifugal force. It has the advantage that several columns can be run contemporaneously in the centrifuge rotor. Classic spin column- grounded DNA and RNA sanctification uses silica membrane columns to insulate nucleic acid from cell, towel, blood, and other sample types. DNA or RNA is bound to silica using high attention of a chaotropic swab similar as guanidine hydrochloride.

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Related Questions

The Embroyblast Eventually Differentiates Into The
A. Zygote
B. Blastocyst
C. Primary Germ Layers
D. Sperm And Egg

Answers

Eventually, the embryoblast differentiates into the zygote.

What differentiates from the embryoblast?

Epiblast and hypoblast are formed during differentiation of the embryoblast. They are collectively known as the bilaminar disc. The procedure starts around the eighth day of pregnancy. The anterior visceral endoderm will be formed by some hypoblast cells.

Does the embryoblast develop into the child?

It has an inner cell mass (ICM), also termed an embryoblast, which later develops into an embryo, as well as a layer of trophoblast cells on the outside known as the trophectoderm. This layer encloses the inner cell mass and the blastocoel, a hollow filled with fluid.

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What element is NOT an essential nutrient for a microbe?a.nitrogenb.carbonc.irond.hydrogene.phosphorus

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Hydrogen is not considered an essential nutrient for microbes. Option D is correct.

Microbes require various elements and compounds to carry out their metabolic processes and sustain life. Carbon, nitrogen, phosphorus, and iron are all essential nutrients for most microbes. Carbon is needed for building the organic molecules that make up the cells, nitrogen and phosphorus are important components of nucleic acids and proteins, and iron is involved in various enzymatic reactions.

While hydrogen is a component of many biomolecules, it is not typically considered an essential nutrient for microbes because it is abundant in most environments and readily available to microorganisms. Microbes can obtain hydrogen through various metabolic pathways, such as fermentation, respiration, and photosynthesis.

Overall, while different microbes have different nutrient requirements depending on their metabolic capabilities, carbon, nitrogen, phosphorus, and iron are generally considered essential nutrients for most microbial growth and metabolism.

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The mushroom poison muscarine can bind to receptors on ________. A) all effector cells stimulated by postganglionic cholinergic fibers B) the hormone producing cells of the adrenal medulla C) cell bodies and dendrites of parasympathetic postganglionic neurons D) cell bodies and dendrites of sympathetic postganglionic neurons

Answers

All effector cells triggered by postganglionic acetylcholine fibers have receptors that the deadly mushroom toxin muscarine can attach to.

The answer to the question is A.

What are cells called?

The majority of all living organisms are made up of cells. The human body is made up of trillions of cells. They not only absorb nutrients from food and turn those carbohydrates into energy, but also give the body frame and carry out certain functions.

What are cell's fundamentals?

A cell is a collection of cytoplasm that has a cell membrane surrounding it on the outside. All living things are made up of cells, which are the lowest structural components of living matter and therefore are typically microscopic in size. The majority of cells have a or more nuclei as well as other organelles that perform a range of functions.

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Which of the following metabolic pathways is common to both aerobic and anaerobic processes of sugar breakdown? t of Select one: A. the citric acid cycle lestion o B. the electron transport chain O C. glycolysis o D. conversion of pyruvic acid to lactic acid

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Glycolysis is metabolic pathways is common to both aerobic and anaerobic processes of sugar breakdown

Both anaerobic and aerobic conditions can result in glycolysis. Pyruvate enters the citric acid cycle under aerobic conditions and proceeds through oxidative phosphorylation, which results in the net synthesis of 32 ATP molecules.

Without the aid of an external electron acceptor like oxygen, glucose is broken down to pyruvic acid through a process called glycolysis. It happens within the cytoplasm of the cell, making it a part of both aerobic and anaerobic organisms.

Because glucose is the only source of energy and glycolysis is an oxygen-free process, it occurs during both aerobic and anaerobic respiration. Hence, this process, which is similar to both aerobic and anaerobic respiration, is unaffected by the presence or absence of oxygen.

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Classify each of the following simple carbohydrates as disaccharides or monosaccharides.1. Fructose2. Lactose3. Sucrose4. Maltose5. Glucose

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Monosaccharides and disaccharides are two categories for simple carbohydrates. Glucose, fructose, and galactose are all monosaccharides, which are compounds that only have one molecule. Lactose, maltose, and sucrose are disaccharides because they each have two molecules.

Is fructose a simple sugar?

Fructose, glucose, and lactose are examples of simple sugars (or carbs), and they are also present in wholesome whole fruits. Starchy vegetables, whole grains, rice, breads, and cereals are examples of foods that include complex carbohydrates (also known as starches).

Maltose is either simple or complicated.

Monosaccharides (glucose, galactose, and fructose) and disaccharides are examples of simple carbohydrates (sucrose, lactose, and maltose). The polysaccharides starch (amylose and amylopectin), glycogen, and fiber are examples of complex carbohydrates.

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Need help with this

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Answer:

I know for sure cigarette smoke, asbestos, but I'm not sure about the other two.

Explanation:

A measure of the strength of the relationship between individual differences in a given trait and individual genetic differences is called the?Heritability Coefficient

Answers

The heritability coefficient is a measure measuring the strength of the link between individual genetic variants and personality factors in a particular trait.

When determining how much of an individual's variation in a trait may be attributable to genetic variances, the term "heritability" is employed. Pay attention to how it sounds. The percentage of variance in any population trait that may be attributable to heritable gene factors (HAYR-ih-tuh-BIH-lih-tee). Estimates of heritability are frequently given in percentages and range between 0 to 1. Genetic variation divided by total phenotypic variance represents heritability, the relative contribution of genetic effects to trait variation. Character is a heritable trait that differs from person to person. The correlation coefficient gauges how strongly a variables (Y) and an independent variable (X).

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What is the role of mitochondria in red blood cells?

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The body's process of producing new blood cells, known as hematopoiesis, is aided by mitochondria, which are typically known for their involvement in the creation of energy in cells.

Recent study from Northwestern Medicine published in Nature Cell Biology has demonstrated this. Both mitochondria and nuclei are absent from the red blood cells (erythrocytes) of mammals. As there are no mitochondria, oxygen is not used by the RBC and is instead transferred directly to the places that need it.

Moreover, RBC lacks other organelles such the Golgi apparatus and endoplasmic reticulum. Mature red blood cells are unable to produce energy through the Krebs cycle (oxidative) because they lack nuclei and mitochondria. Erythrocytes instead rely on the Embden-Meyerhof pathway's anaerobic glucose conversion to produce and store high-energy phosphates.

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inner mass cells are derived from the blastocyst and are considered ______

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A blastocyst is made up of an inner group of cells with an outer shell. The inner group of cells will become the embryo.

What is the embryo made of?

The fertilized egg cell divides into two cells. Each cell then divides into two additional cells and so on until, after a few days of cell division, a tiny embryo develops. In the early stages, the microscopic embryo is made up of cells that have the potential to develop into all types of cells.

Where is embryo located?

Uterus. The uterus, or womb, is a hollow, pear-shaped organ ln a woman's lower stomach between the bladder and the rectum. It sheds its lining each month during menstruation.

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What is the difference between exocrine vs endocrine glands?

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The primary distinction between the two is that exocrine glands discharge substances into a ductal system to an epithelial surface, whereas endocrine glands secrete products directly into the bloodstream.

Exocrine gland should be defined.

An exocrine gland is a gland that secretes on the surface or into a specific location via ducts in order to execute a metabolic activity.

Define the term endocrine glands.

Ductless glands are endocrine glands. They emit hormones that aid in the coordination of our activities. These hormones are secreted into the bloodstream and circulate throughout our bodies.

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mitosis, followed by cytokinesis, results in the formation of two genetically identical daughter cells. this is because

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Mitosis, followed by cytokinesis, results in the formation of two genetically identical daughter cells because: all the genetic material is duplicated then segregated equally to the daughter cells.

Do all of the daughter cells share the same genetic makeup?

Daughter cells that really are genetically similar to their parent cells are created during mitosis.The cell divides its copied chromosomes evenly to ensure that each cell has a full set before copying, or "replicating," its chromosomes.

Which stage of mitosis ensures that the genetic material of daughter cells is distributed equally?

However, the duplicated chromosomes must be properly arranged before being divided and equally distributed to the two daughters during mitosis, and this process starts in the S phase.

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moss invades and establishes itself on bare rock, accumulating the beginnings of soil. after several years, enough soil has become established that grasses begin to grow where there was once bare rock. without the moss building up soil, the grasses would have had no chance. the mosses changed the environment enough to permit grasses to grow in a process called

Answers

The process by which the mosses change the environment enough to permit the growth of grasses is called succession or ecological succession.

Ecological succession refers to the gradual change in species composition and structure of an ecosystem over time. In the case of moss invading bare rock and establishing itself, the mosses accumulate the beginnings of soil and create a new environment that supports the growth of new species, such as grasses.

As the ecosystem continues to change and mature, other species may establish themselves, leading to a shift in the dominant species and a continuation of the process of succession.

Succession is a natural process that occurs in ecosystems and can be influenced by many factors, including climate, geology, and biotic interactions. The ultimate goal of succession is to reach a stable and mature ecosystem, known as the climax community, which is characterized by a stable and diverse mixture of species that are well-adapted to the local environment.

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What is the ICD-10-CM code for uterine fibroid?

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Leiomyoma of the uterus, undetermined is medically categorised as an ICD-10 code D25. 9 under the heading "Neoplasms" by the World Health Organization.

Noncancerous uterine growths known as uterine fibroids are common during the  travail times. Uterine fibroids, also known as leiomyomas or myomas, do not enhance the  threat of uterine cancer and hardly ever turn into the  complaint.  ICD-10-CM  law D26.9, which is billable/ specific, can be used to denote a  opinion for  fiscal payment.

Uterine fibroids come in three different main  orders. Intramural fibroids develop inside the uterine wall's musculature. The uterine  depression is  beetled into by submucosal fibroids. Subserosal fibroids bag from the uterus's wall. Myomectomy. A myomectomy is an operation to remove fibroids while conserving the uterus. For women who have fibroid symptoms and want to have children in the future, myomectomy is the stylish treatment option. Myomectomy is  veritably effective, but fibroids canre-grow.

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what is the important part in regulating spindle fiber during cell division

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Answer:

The important part in controlling spindle fiber is the centrosome.

Why is 5´ cap and poly-A tail important?

Answers

Eukaryotic messenger RNA (mRNA) molecules have two structural components called the 5' cap and poly(A) tail that are crucial for translation initiation, RNA processing, and gene expression.

The freshly produced mRNA molecules have a modified guanine nucleotide called the "5' cap" appended to their 5' ends.

The 5' cap is essential because it aids in shielding the mRNA from exonuclease degradation, improves translation efficiency by encouraging the recruitment of ribosomes to the mRNA, and makes splicing easier by designating the beginning of the coding sequence.

Adenosine nucleotides are attached to the 3' end of mRNA molecules to form the poly(A) tail.For mRNA degradation and mRNA export,the poly(A) tail is very essential.

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the random movement of molecules down their concentration gradient is called______

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The random movement of molecules down their concentration gradient is called diffusion.

What is diffusion?

Physically, diffusion is the movement of particles or molecules from a high-concentration region to a low-concentration one. This movement happens as a result of the molecules' or particles' random motion, and it persists until the particle concentration is uniform throughout the system. The dispersion of signalling molecules throughout the body and the exchange of gases in the lungs are just a few examples of the numerous natural processes that depend on diffusion. The movement of atoms, ions, and molecules in diverse materials and systems is explained using this crucial idea in chemistry and physics as well.

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What is the difference between leading strand and lagging strand synthesis? Which strand requires just one primer? Which strand requires multiple primers? Are the primers RNA or DNA?

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Leading and lagging strand synthesis are two processes that occur during DNA replication. The leading strand is the strand that is synthesized continuously in the 5' to 3' direction, while the lagging strand is the strand that is synthesized discontinuously in the 5' to 3' direction, forming short Okazaki fragments.

The leading strand requires just one primer, which is usually synthesized by the enzyme primase. This primer is made of RNA, and it provides the 3' end for DNA polymerase to start synthesizing the new strand.

The lagging strand, on the other hand, requires multiple primers because DNA polymerase can only add nucleotides in the 5' to 3' direction. As the replication fork opens, new primers are required to initiate the synthesis of each Okazaki fragment.

Both primers on the leading and lagging strands are made of RNA, not DNA. The primers are complementary to the DNA template strand and provide a free 3' OH group for DNA polymerase to extend the new strand. Once the RNA primers are laid down, they are eventually replaced by DNA nucleotides through the process of DNA synthesis.

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pacinian corpuscles are sensory receptors that respond to

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Pacinian corpuscles are rapidly-adapting, deep receptors that respond to deep pressure and high-frequency vibration.

What is the function of corpuscles?

Meissner corpuscles consist of a cutaneous nerve ending responsible for transmitting the sensations of fine, discriminative touch and vibration. Meissner corpuscles are most sensitive to low-frequency vibrations between 10 to 50 Hertz and can respond to skin indentations of less than 10 micrometers.

What color are corpuscles?

It is to the aggregation of the red corpuscles that the blood owes its red hue, although when examined by transmitted light their color appears to be only a faint reddish yellow. The corpuscles vary slightly in size even in the same drop of blood, but the average diameter is about 7.5μ,  and the thickness about 2μ.

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What role does nondisjunction play in the formation of a fertile allopolyploid hybrid?
A. It doubles the chromosome number in the hybrid. B. It allows gametes of the two parent species to combine. C. It causes the chromosomes to line up at the center of the cell during mitosis. D. It breaks down chromosomes that don't exist as homologous pairs.

Answers

Nondisjunction plays a role in the production of a viable allopolyploid hybrid by doubling the number of chromosomes in the hybrid. Option A is correct.

Nondisjunction arises when chromosomes fail to separate during meiosis, resulting in gametes with an incorrect number of chromosomes. Nondisjunction occurs when homologous chromosomes fail to disjoin appropriately during meiosis. This leads in the creation of gametes with more or less chromosomal content than usual. As a result, the individual may have a trisomy or monosomy syndrome.

Nondisjunction occurs when a pair is homologous chromosomes fails to split or segregate during anaphase, causing both chromosomes to pass to the same daughter cell. This is most likely to happen during meiosis, but it might also happen during mitosis to form a mosaic person.

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Classify each description as true of introns only, true of exons only, or true of both introns and exons. Answer Bank generally absent from bacterial genomes present in eukaryotic genomes code for a protein

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Sort each statement into whether it applies to exons only, introns only, or both exons and introns. Imagine that an intron-rich eukaryotic gene was being translated by RNA polymerase.

An eukaryotic cell is what?

The Eukaryotic Cell The phrase "Eukaryotes" derives from the Greek words "eu," which means "good," and "karyon," which means "kernel," and so means "good or real nuclei." Prokaryotes are substantially smaller and less complicated than eukaryotes. All except one of the main kingdoms are represented by them.

What distinguishes prokaryotic and eukaryotic organisms?

The lack of a nucleus distinguishes prokaryotic cells as a primordial kind of cell. Prokaryotes also lack cellular organelles that are attached to membranes. Prokaryotes are only monocellular organisms. A eukaryotic cell is what? Eukaryotic cells are those that have a real nucleus and organelles that are attached to membranes.

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True or False? Cartilage has a flexible matrix that can accommodate mitosis of chondrocytes – this makes it an excellent scaffolding tissue upon which to build bone.

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Is it true because cartilage is a type of connective tissue that has a flexible matrix made up of collagen and proteoglycans.

The matrix provides the tissue with its shape and mechanical properties. Within the matrix, there are chondrocytes, which are the cells responsible for producing and maintaining the matrix.

Cartilage has the ability to accommodate mitosis of chondrocytes, which means that the cells can divide and multiply within the matrix. This property makes cartilage an excellent scaffolding tissue upon which to build bone. During bone development, the cartilage matrix serves as a template for the deposition of bone tissue. As the bone tissue is deposited, the cartilage matrix is gradually replaced, leaving behind a structure that is strong and durable.

In summary, the flexible matrix and ability to accommodate mitosis of chondrocytes make cartilage an excellent scaffolding tissue upon which to build bone.

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If the sperm cell of an organism contains 14 chromosomes, how many chromosomes are in an autosomal cell of this organism.

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If a sperm cell of an organism contains 14 chromosomes, the number of chromosomes in an autosomal cell of this organism is 28.

How so many clones would a 14-chromosome cell produce?

Mitosis is a kind of cell division that produces two daughter cells with the same number of chromosomes as the parent cell.The two daughter cells will have 14 chromosome each if the mother cell had 14 chromosomes.

How many chromosome 14 copies are there?

Humans typically have two copies of their 46 chromosomes, which are split into 23 pairs.One of the pairings is made up of two copies of chromosomes 14, one from each parent.

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the primary mirror of telescope a has a diameter of 20 cm, and telescope b has a diameter of 100 cm. how do the light gathering powers of these two telescopes compare?

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The capacity of telescope B to gather light is 25 times that of telescope A. An 8-inch telescope can identify over 2000 times quite so many planets (10 million opposed to 5000) than the human eye since there are far more dim stars than dazzling ones.

Formulae. Magnifying power equals (object focal length) / (p(diameter of objective)2/4) (eyepiece focal length). It's very important to remember that when a telescope's size is doubled, it really collects four times as much light instead of two since the optical surface area increases as the squared of the aperture. Thus, an 8-inch telescope can gather four times as much light as a 4-inch telescope. 1600 times more light can be collected by an 8-inch telescope than by the human eye. An 8-inch telescope can identify over 2000 times quite so many planets (10 million opposed to 5000) than the human eye since there are far more dim stars than dazzling ones.

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in the sense of alternation of generations, how many generations are included in a mature pine seed?
a. two generations of tissues
b. three generations of tissues
c. one generations of tissues

Answers

In the sense of alternation of generations, two generations of tissues are included in a mature pine seed. Therefore, the correct option is A.

What is alternation of generation?

Alternation of generations is a type of life cycle that is characteristic of many plants and some algae, where the organism alternates between two distinct multicellular generations: a haploid (n) gametophyte generation and a diploid (2n) sporophyte generation.

In the life cycle of pines, the sporophyte generation produces cones that contain haploid spores. The male gametophytes produce sperm, while the female gametophytes produce eggs. Fertilization between the sperm and egg results in the formation of a diploid zygote, which develops into the sporophyte generation.

In the sense of alternation of generations, a mature pine seed contains tissues from both the sporophyte and gametophyte generations. Therefore, the correct option is A.

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A shielded nucleus will absorb ______ from a deshielded nucleus and will have a ______ chemical shift.

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A shielded nucleus will absorb upfield from a deshielded nucleus and will have a lower chemical shift.

What in NMR is shielded or deshielded?

When the electron density around the nucleus is high in NMR, the magnetic field that opposes the electrons is likewise increased, providing significant shielding. Deshielding occurs when the nucleus's surrounding electron density decreases, the magnetic field that is obstructing it weakens, and the nucleus begins to sense more of the external magnetic field.

Radiation shielding is the insertion of a protective shield between an ionizing radiation source and the object to be protected in order to lessen the potential harm to sensitive or biological components, particularly during transport.

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When we pop a synovial bubble, the bubble is filled with nitrogen gas where does this nitrogen gas come from

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Answer:

Nitrogen gas in joints

Escaping gases: Scientists explain that synovial fluid present in your joints acts as a lubricant. The fluid contains the gases oxygen, nitrogen, and carbon dioxide. When you pop or crack a joint, you stretch the joint capsule. Gas is rapidly released, which forms bubbles.

the forelimbs of mice, bats, and whales all have a similar bone structure. this is an example of which type of evidence of shared ancestry?

Answers

The example of shared ancestry depicted by the forelimbs of mice, bats, and whales all having a similar bone structure is of: homologous structures.

Homologous structures are the those organs or body parts that exist in different species but have common structure. This indicates that these species evolved from a common ancestor. The homologous organs may adapt to different functions according to the species.

Ancestry is defined as the origin or the roots of different living organisms. If two or more species have a shared ancestry this means that they originated from a common ancestor. Thus, people having common ancestors have genetic relationships and thus have similar structures.

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what base pairs with the codon on the mrna molecule?

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The aminoacyl-tRNA anticodon base pair up with the codon present on the mRNA molecule.

During the process of translation, the large subunit of the ribosome contains three sites basically at which the tRNA molecules is able to bind. The A (which is amino acid) site is the location where the aminoacyl-tRNA anticodon base are able to pair up with the mRNA codon, and this happens to ensure that the correct amino acid is being added to the polypeptide chain which is growing.

The P (which is the polypeptide) site is basically the location where the amino acid gets transferred from the tRNA onto the growing polypeptide chain. Finally, the E site or the exit site is where the empty tRNA is present before it gets released back into the cytoplasm.

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what is the moveable end of the muscle

Answers

Insertion is the moveable end of the muscle that attaches to the bone being pulled.

Any muscle generally has two ends- one moveable and the other fixed. The fixed end is attached to a stabilized bone and is called origin, while the free end, which is moveable, is attached to the bone being pulled. The free and moveable end is, therefore, called the insertion.

There are generally three types of muscles- skeletal, smooth, and cardiac muscles. The muscles responsible for any movement in the body are the skeletal muscles. This is why they are also called the voluntary muscles. each muscle joins the bones at various points through tendons.

Other than skeletal muscles, smooth muscles are responsible for regulating involuntary functions. The cardiac muscles are only found in heart and regulate its movements.

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Does anyone know what the order is?

Answers

Samples were treated with chemicals to release DNA from cells

Different restriction enzymes were used to produce DNA fragments

DNA fragments were combined to produce recombinant DNA

Recombinant DNA was inserted into bacteria for storage and sequencing

What is the human genome?

The human genome aims at being able to get together all the genes taht can be found in the human body. This is a large project that have been in progress for quite some time.

At the completion of the project, the sequence of all the DNA that can be found in the human  begin would have been successfully obtained.

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