You have a piece of DNA containing the gene for green fluorescent protein (gfp) and the pBAD promoter. This DNA fragment has been cleaved with BamHI and HindIII and can be inserted into either the pKAN-R plasmid or the pARA plasmid. Which plasmid would you choose to use as the plasmid vector to express gfp protein in bacterial cells

Answers

Answer 1

Answer:

pARA plasmid

Explanation:

Plasmids are natural circular DNA samples found in bacteria cells. There are two types of plasmids founds in bacteria cells namely; pARA and pKAN-R.

The pKAN would not be maintained in the cell during replication but the pARA plasmid would and when the DNA fragment is inserted, the pARA would serve as a catalyst for the gfp protein.


Related Questions

Differences between prokaryotic cells and eukaryotic cells

Answers

Answer:

eukaryotic cells have a membrane-bound nucleus and prokaryotic cells do not.

Explanation:

Eukaryotes have many membrane-bound organelles while prokaryotes have no membrane-bound organelles.


Which procedure took the DNA from one sheep to produce a genetically identical lamb, named Dolly?
selective breeding
gene therapy
artificial selection
cloning

Answers

Answer:

Cloning

Explanation:

If you take DNA and make a genetically identical thing with that DNA it would be cloning

Answer

D cloning

Explanation:

i just took the quiz and made a 100

What type of energy is formed when chemical bonds are broken?
A)Thermal energy
B)Chemical energy
C)Radiant energy
D)Light energy

Answers

Answer:

option A.thermal energy  because if a  bond breaks it loses energy in the form of heat which is thermal energy.

Explanation:

Answer:

ik im late but its chemical energy

Explanation:

Which type of soil would be most suitable for rice?

Answers

Answer:

Slit Clay

Explanation:

Rice needs a lot of water to grow and clay holds water.

HELP ASAP brainliest if correct
A scientist conducting an experiment quickly saw that she was not getting the results she expected. Instead of continuing to collect data, she went back to redesign the experiment until the data came out the way that the hypothesis predicted. The scientist’s work did not follow the scientific method. Which statement best explains why?

A: She followed the steps of the scientific method, but in the wrong order.

B: She made improvements to her experimental design rather than keeping it the same.

C: She failed to look at the data objectively by trying to get a desired result.

D: She carried out multiple trials rather than carrying out one trial correctly.

Answers

Answer:

C

Explanation:

Because she didnt observe long enough and things can always change

C) she failed to look at the data objectively be trying to get a desired result

PLEASE RESPOND IF YOU KNOW AND QUICK <3
1.
All biomolecules are made up of the elements(1 point)

A-carbon, hydrogen, nitrogen, and oxygen.

B-carbon, hydrogen, and oxygen.

C-carbon, hydrogen, nitrogen, oxygen, and sulfur.

D-carbon, hydrogen, oxygen, and sulfur.

2.
Nucleic acids are the building blocks of(1 point)

A-DNA.

B-proteins.

C-nucleotides.

D-biomolecules.

3. One of the jobs of lipids is to act as chemical messengers. This job is particularly important for the function of the(1 point)

A-brain.

B-lungs.

C-stomach.

D-heart.

4.
A complex carbohydrate with long strands of glucose is known as a(1 point)

A-multisaccharide.

B-trisaccharide.

C-saccharide

D-polysaccharide.

5.
What makes amino acids unique from fatty acids and sugars?(1 point)

A-Amino acids are building blocks to larger molecules; fatty acids and sugars are not.

B-Amino acids contain nitrogen; fatty acids and sugars do not.

C-Genetic material is made of nucleotides, which contain amino acids; fatty acids and sugars are not part of genetic material.

D-Amino acids make up proteins, which provide energy for the body; fatty acids and sugars do not provide energy.

Answers

Answer:

1. A. carbon,hydrogen,nitorgen and oxygen

2.C. Nucleotides

3.A.Brain

4.D. Polysaccharide

5. B.Amino acids contain nitrogen; fatty acids and sugars do not.

Many compounds that are of biological importance are comprised of carbon, hydrogen and oxygen and other heteroatoms such as sulfur, nitrogen etc.

The correct answers are as follows;

1) The correct answer is B: carbon, hydrogen, and oxygen

2) The correct answer is A: DNA

3) The correct answer is A: brain

4) The correct answer is D: polysaccharide

5) The correct answer is B: Amino acids contain nitrogen; fatty acids and sugars do not

Biomolecules are molecules that are useful to in biochemical processes.

Several biomolecules are known and are of great importance in the body as they facilitate life.

Most of these biomolecules contain carbon, hydrogen, and oxygen.

A prominent group of biomolecules are the nucleic acids which are composed of nucleotides. They come together to form DNA.

Lipids are another category of biomolecules which are important components of hormones which serve as chemical messengers in the body and regulate the activities of the brain.

Glucose are biomolecules which come together to give larger molecules called polysaccharide.

Both fatty acids and sugars are biomolecules. Fatty acids and sugars all contain carbon, hydrogen and oxygen but not nitrogen. Amino acids are different from fatty acids and sugars because they contain nitrogen.

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PLEASE ANSWER THIS AND THANKS TO WHO EVER WILL ANSWER IT AND IF YOU GIVE ME A COMPLETE ANSWER THEN ILL MARK YOU BRAINLIEST

Answers

Hey can you please take a better picture? It’s a little blurry!
1: seafood, lean meat, poultry.

Green plants can make glucose.

Plants need energy to make glucose.

How do plants get this energy?​

Answers

Answer:

atp or solar energy or from the sun

Explanation:

Amy's
's oven always under cooks her cakes. What could be a solution to develop a better oven?

Answers

Answer:yes

Explanation:

Imagine a field of flowers

Answers

Answer:

Great, just did. Is there more to the question?

Explanation:

Answer:

I love just going to an open field with a bunch of flowers with cousin it's so relaxing just laying there not caring one bit about the rest of the world sharing secret laughing and then peaceful silence

Explanation:

List two species that belong to the same Kingdom, Phylum and Class.(Site 2)

Answers

Answer:

The image has your answer :)

Explanation:

How does the respiratory system interact with the circulatory system?
A. The respiratory system brings in carbon dioxide so the circulatory system can transport it throughout the body.
B. The circulatory system transports oxygen that the respiratory system brings into the body.
C. The respiratory system transports oxygen to the heart for the circulatory system.
D. The circulatory system removes oxygen from the body to make room for carbon dioxide in the respiratory system.
Help!!

Answers

Answer:B

Explanation:has nothing to do with carbon dioxide and does not bring oxygen to the heart it goes to the lungs.

what is budding in plants

Answers

Answer:

Grafting and budding are horticultural techniques used to join parts from two or more plants so that they appear to grow as a single plant.  In the budding process, a bud is taken from one plant and grown on another. Although budding is considered a modern art and science, grafting is not new.

Explanation: Cause seeds reduce its form and plant

Btw have nice rest your night

Asexual reproduction -> cell division -> outgrowth

ex. found in yeast, hydra

The algae at the beginning of the food chain below are?
A
Consumers
B.
Producers
C.
Decomposers
D
Heterotrophs

Answers

Answer:

Algae is a primary producer

Explanation:

they use inorganic ions, co2, h2o and solar energy the complex organic molecules are produced. They release oxygen in this process. Algae, cyanobacteria and plants indirectly produce all food and all oxygen for all living organisms on our planet

PLS GIVE A REAL ANSWER ILL MARK BRAINLIEST!! in the three stages of the cell cycle, (interphase, mitosis and cytokinesis) which organelles are affected in the cell?

Answers

Answer:

nucleus, mitotic spindle and microtubules.

Explanation:

Organelles that are involved in cell division :

Key organelles that make mitosis go as planned include the nucleus, mitotic spindle and microtubules.

Why is an electromagnet a temporary magnet?

Answers

Answer:

An electromagnet is a temporary magnet. It is made by winding a coil of insulated wire round a soft iron core. When a current is passed through the coil, the magnetic field produced by the current magnetises the soft iron core. The soft iron core loses all the magnetism when the current is switched off.

Explanation:

yes

Answer:

The magnetic field generated by the electromagnet only appears when there is an electrical current applied to it. The magnet field will disappear once the electrical current has left.

PLEASE HELP
Decomposition is the process by which:
Carbon atoms are released into the soil
Carbon is converted to CO2
Organisms obtain energy by breaking down glucose



Answers

Answer:

carbon is converted into co2.

Explanation:

Explain how carbohydrates break down

Answers

Answer:

Digesting carbohydrates involves first the starch and glycogen breaking down into glucose by amylase and maltase. sucrose and lactose are broken down by sucrase and lactase

What is weight measured in

A grams

B Kilograms

C Metric Tons

D pounds

Answers

Concept: Unit Representation

You have weight which is commonly represented in SI units of KG In the US metrical units are poundsYour answer depends on which metric you are referring to.
All of these are a measurement of weight

What is a catalyst and how do they help with the chemical reactions needed for metabolism?

Answers

Answer:

See explanation

Explanation:

A catalyst refers to any substance that increases the rate of a chemical reaction. A catalyst does not affect the equilibrum position rather it enables the system to attain equilibrum faster.

Biochemical catalysts increase the rate of biochemical reactions in the body. They enable the body to carry out certain important functions that are necessary for life.

When you surprised that your mom is on the Hospital and she have a lung cancer stage4 what should you do?​

Answers

Answer:

what you can

Explanation:

my grandma just got diognosed with two different types of lung cancer, stage 3. Not much you can do but be there for her. One thing that we did for her was make her a cancer/chemo basket after doing research. Apparently it tastes gross so you give them hard candy to suck on and there are certain things like ginger flavored ones or ginger smelling sticks that make you not be nasaus and hats in case she loses her hair (which they said she wouldn't) and a blanket with all of the family on it so she never has to be alone and stuff like that. They're aloud to take one bag to chemo so I would really reccommend doing it. She loved it anyway.

Hope everything turns out ok *sad smile*

What is the function of the polar bodies created during the meiosis of the female egg cell?

Answers

Answer:

Polar bodies serve to eliminate one half of the diploid chromosome set produced by meiotic division in the egg, leaving behind a haploid cell.

Explanation:

In eukaryotic cells, proteins are made in the

a
nucleus
b
cytoplasm

Answers

Answer:

nucleus is responsible for proteins

The insulated feature of space suit layers are designed to - A protect from radiation. B trap excessive CO2. C regulate temperature. D provide pure O2.

Answers

Answer:

C. regulate temperature

Explanation:

Answer:

c

Explanation:regulate temperature

name the function of water in saliva

Answers

Answer:

Moistening food and helping to create a food bolus so food can be swallowed easily

On one cactus species, the red flowers are dominant over white flowers. If you crossed two heterozygous cacti, what would be the probability of having offspring with red flowers?

Answers

Answer:

3/4

Explanation:

This question involves a single gene coding for flower color in cactus plant. The allele for red flowers (R) are dominant over the allele for white flowers (r). This means that a cactus that is heterozygous (Rr) for the Gene will possess a red phenotype.

In this cross, two heterozygous cacti are crossed. The following gametes will be produced by each heterozygous parent: R and r. Using these gametes in a punnet square, the following genotype of offsprings will be produced;

RR, Rr, Rr, and rr

RR, Rr and Rr are Red flowers (3/4)

rr are white flowers (1/4)

Therefore, the probability of having offspring with red flowers is 3/4.

The enzymes catalyzing the reactions of glycolysis occur in the:

Answers

The occur in the cell structure of the cytoplasm

What would happen is tRNA did not work properly?

Answers

Ribosomes provide a structure in which translation can take place. ... If you're not yet familiar with RNA (which stands for ribonucleic acid), I highly recommend ... We'll learn a lot more about tRNAs and how they work in the next section. ... How does the right amino acid get linked to the right tRNA (making sure that codons ...

Hurry im giving 50 point

Which prediction is most likely when the air pressure increases?

A.There will soon be days of thunderstorm B.Rain is on the way. C.Sunny, clear skies are coming. D.Snow will fall, and then rain will follow.

Answers

Answer:

c

Explanation:

Describe three levels of biodiversity

Answers


B
nba youngboy
is the goat

The three levels of biodiversity are genetic diversity, species diversity, and ecosystem diversity.

Genetic Diversity: Genetic diversity refers to the variety of genes within a species. It includes the different alleles and variations present in the gene pool of a population. Genetic diversity is important because it provides the raw material for adaptation and evolution. A higher genetic diversity within a species increases its ability to survive and thrive in changing environments. It also helps in disease resistance and overall population health.

Species Diversity: Species diversity refers to the variety of different species present in a particular area or ecosystem. It measures the number of different species and their relative abundance. Higher species diversity indicates a healthier ecosystem and greater ecological resilience. Each species plays a unique role in the ecosystem and contributes to its stability and functioning. Loss of species diversity can disrupt ecosystem balance and lead to negative impacts on other species and ecosystem processes.

Ecosystem Diversity: Ecosystem diversity refers to the variety of different ecosystems or habitats within a region or on a global scale. It encompasses the different types of ecosystems such as forests, grasslands, wetlands, coral reefs, and more. Each ecosystem has its own set of species, interactions, and ecological processes. Ecosystem diversity is essential for maintaining ecological functions, nutrient cycling, and providing various ecosystem services such as clean air, water, and food. It also supports the resilience and adaptability of ecosystems to environmental changes.

These three levels of biodiversity are interconnected and mutually dependent. They provide the foundation for the functioning and sustainability of our planet's ecosystems. Protecting and conserving biodiversity at all levels is crucial for the well-being of both human populations and the natural world.

To learn more about biodiversity, here

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