The gel below shows a result of the experiment you designed using the DNA of three different individuals (S1-S3). It also shows a negative (C-) and a positive (C ) control, as well as a DNA marker. Why did S2 have three bands

Answers

Answer 1

Restriction enzymes recognize specific nucleotidic sequences. The molecule with no recognition site will not be digested. Individual S2 probably had molecules with the recognition site and others with a different sequence.

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A restriction enzyme is a degradative polymer that recognizes and cuts up specific nucleotide sequences in DNA.

Restriction enzymes can produce cohesive cuts or blunt (abrupt) cuts, depending on the recognition sites.

The frequency in which the restriction enzyme will produce the cuts depends on the recognition sequences and the number of bases that compose them.

So restriction enzymes cut DNI molecules into different short sequences of varying lengths.

These pieces are called minisatellites and exhibit the existing variation from one person to the next.

These short sequences can be separated by using gel electrophoresis and will express a pattern of differentiation.

When the nucleotidic sequence of the DNA molecule does not include the recognition site, the enzyme will not cut this fragment. The enzyme does not digest this molecule.

Since the negative control does not have a recognition site, it is not digested by the enzyme.

The positive control is digested, meaning that these molecules carry the recognition site.

Probably DNA molecules from individual S1 do not have the recognition site either.

The enzyme recognizes the sequence in DNA from individual S3 and cuts it into two fragments.

The lane of Individual S2 includes both options: digested and not digested DNA. This means that the sample taken from this individual included DNA

                               ⇒  carrying the sequence, and

                               ⇒  not carrying the recognition sequence.

So enzymes digest molecules carrying the recognition sequence, and the other ones remain undigested.

Probably, individuals S1 and S3 are h0m0zyg0us, while individual S2 is heter0zyg0us.

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Related Questions

Question 1
Alberto created a graph to illustrate his trip. Answer the questions below.
A. How does Alberto's motion change at point B?
B. What is Alberto's speed from point C to point D?
C. What happens at point D? (1 point)

Question 2
Answer the question below about Smokey The Mouse.
A. Use inertia to explain how a balanced force will affect his motion. (Newton's 1st Law)
B. What are two ways Smoky can increase his speed? (Use Newton's 2nd Law to help you)
C. What is an example of an action and reaction at work in this picture? (Use Newton's 3rd Law to help you)

Answers

The mouse exerts a force on the ground and the ground exerts an equal and opposite reaction force on the mouse.

At point B, Alberto's distance covered increases with time, equal distance is covered in equal time interval. The speed increases uniformly. At the point C to D, the distance covered with time remains the same. At the point D, equal distance is no longer covered in equal time intervals.

According to the law of inertia, an object will remain at rest or in a state of uniform motion unless it is acted upon by an unbalanced force. This means that smokey mouse will continue moving uniformly unless a force acts on it.

From Newton's second law; F = ma and a = F/m. F = force , m= mass, a = acceleration. This implies that smokey mouse can increase its speed by;

Increasing the force acting on the mouseDecreasing the mass of the mouse

Action and reaction is encountered as the mouse is walking. The mouse exerts a force on the ground and the ground exerts an equal and opposite reaction force on the mouse.

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What are the Acute myeloid Leukemia stages?

Answers

Answer:

M0: undifferentiated acute myeloblastic leukemia.

M1: acute myeloblastic leukemia with minimal maturation.

M2: acute myeloblastic leukemia with maturation.

M3: acute promyelocytic leukemia.

M4: acute myelomonocytic leukemia.

M4 eos: acute myelomonocytic leukemia with eosinophilia.

M5: acute monocytic leukemia.

Explanation:

Stage 0. The blood has too many white blood cells called lymphocytes. This is called lymphocytosis. ...

Stage I. The blood has too many lymphocytes. ...

Stage II. The blood has too many lymphocytes. ...

Stage III. The blood has too many lymphocytes. ...

Stage IV. The blood has too many lymphocytes.

both works hope this helps.

1:Can all genetic mutations be observed with a karyotype? Explain:
2:Evaluate the use of karyotyping in genetic testing to determine the health of an unborn baby.

Answers

Explanation:

Karyotyping is a test to examine chromosomes in a sample of cells. This test can help identify genetic problems as the cause of a disorder or disease.

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the process of ending a conflict through cooperation and compromise is called

Answers

Answer:

the process of ending a conflict through cooperation and compromise is called. conflict resolution. the use of communication and, in many cases, compromise to settle a disagreement is known as. negotiation.

Explanation:

the process of ending a conflict through cooperation and compromise is called. conflict resolution. the use of communication and, in many cases, compromise to settle a disagreement is known as. negotiation.

what are processes of the water cycle that return water to the atmosphere

Answers

Answer:

Evaporation and transpiration change liquid water into vapor, which ascends into the atmosphere due to rising air currents.

help help pls
choose the right letter

29. The ABO blood type gene is an example of which means that heterozygous display two phenotypes at the same time.

A. Fading
B.Incomplete dominance
C. Codominance
D. Advanced hybridism​

Answers

Answer:

Codominance i believe :)

WHAT R THE ORGANSIMS THAT CAN BE SEEN BY LIGHT MICROSCOPE

Answers

Answer:

bacteria, mitochondria, human egg

Lemme add this Amoeba paramecium and euglena

Which molecule is needed for photosynthesis to occur?

glucose

oxygen

carbon dioxide

nitrogen

Answers

Answer:

carbon dioxide

Explanation:

Photosynthesis requires sunlight, carbon dioxide, and water.

A running back is running down the field and two people on the opposing team run towards him. One person is running east with a force of 250 N. The other person is running west with 250 N of force . They all collide at the same time. Show the forces affecting the running back.

Are the forces balanced or unbalanced?

Net force = ?

Answers

Given our understanding of the laws of physics, we can confirm that the Net force affecting the running back (ignoring gravity and its complementary force) is that of 500N and balanced.

In physics, we can say that the net forces being applied to an object are balanced when the forces are

Equal in sizeApplied in opposite directionsApplied at the same time.

The question states that the running back collides with two members of the opposing team at the same time. This, coupled with the fact that the opposing team members are described to arrive on exactly opposite sides, we can say that the forces being applied to the running back are indeed balanced.

Furthermore, since the forces are 250N in size each, we can confirm that the net force applied to the running back is 500N. To this, we would normally add the force of gravity, however, in order to do this, we would need the weight of the running back, that is, data that is not provided.

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Okay so on Mine**craft, I have a world on my ipad. But, I recently downloaded java edition on my macbook. Is there some way I could transfer it to my mac?

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Press “Open World”, select your Bedrock world as the source, then press “Convert” on the left. Press “Select Output World”, then choose the Java world you created. When ready, press “Convert” to begin the process.

I searched it and found this, let me know if it helps!

Which structure is found in all eukaryotic cells?


flagella


large central vacuole


cilia


Golgi apparatus

Answers

Answer:

the Golgi apparatus is the answer

I think it is the Golgi Apparatus

Which butterfly-shaped portion of the brain is responsible for spinal reflexes?

Answers

Answer:

b. Gray matter

Explanation: is correct

how do identify veins in our body​

Answers

Answer:

Veins are blood vessels in humans, and most other animals that carry blood towards the heart. Most veins carry deoxygenated blood from the tissues back to the heart; exceptions are the pulmonary and umbilical veins, both of which carry oxygenated blood to the heart. In contrast to veins, arteries carry blood away from the heart.

Explanation:

Where does the chemical energy to produce ATP come from?

The use of chemicals fro the environment to make sugars

The conversion of ATP to ADP

The breakdown of carbon-based molecules into smaller molecules

The addition of a phosphate group to ATP

Answers

The break down of carbon based molecules into smaller molecules

How much atp is produced in cellular respiration.

Answers

Biology textbooks often state that 38 ATP molecules can be made per oxidized glucose molecule during cellular respiration (2 from glycolysis, 2 from the Krebs cycle, and about 34 from the electron transport system).

I really need help this is carbon cycle help me label it

Answers

Answer:

1.) Photosynthesis

2.) Respiration by plants

3.) Feeding

4.) Death and waste

5.) Respiration by animals

6.) Decomposition

7.) No Decomposition

8.) Combustion

Hope this helps!



Which of the following is a reasonable claim the researchers can make based on the data in the graphs?

Answers

Answer:

First give the options we can't tell random ir may be wrong give options because in question it is asking which of the following if we say any randomly it becomes wrong.

Which of the following help to break down proteins?

Answers

Answer:

b. Pepsin and trysin

Explanation: is correct

B) pepsin and trysin

What does an optical part of the light microscope consist of? A. Ocular.
B. Diaphragm.
C. Condenser.
D. Turret

Answers

Answer-
C. Condenser

What is oxidative phosphorylation.

Answers

Answer:

Oxidative phosphorylation or electron transport-linked phosphorylation or terminal oxidation is the metabolic pathway in which cells use enzymes to oxidize nutrients, thereby releasing chemical energy in order to produce adenosine triphosphate. In eukaryotes, this takes place inside mitochondria

Answer:

Oxidative phosphorylation is energy given by electron transport and this takes place during aerobic respiration in the mitochondria.

Explanation:

This process produces huge amounts of ATP, which is the unit of energy for metabolic processes.

Does “survival of the fittest apply to domesticated animals? Why or why not? *

Answers

Answer:

Yes.

Explanation:

This survival of the fittest is instinct helps domestic animals live. Just because an animal is domesticated, does not mean that instincts go away. Those instincts like hunting may get suppressed, but not taking away.

Answer:

Survival of the fittest does not apply to domesticated animals. This is because these animals don't live in the wild so they don't have to run after things and kill them. Their food comes out of a tin and is provided by human beings. They don't have any predators so their lives are seldom at risk. Thus, they don't need to be fitter or more powerful than other animals in any way.

Lets take the example of pet dogs. We keep them for our support or simply because we enjoy their company. We provide them with food, shelter etc. and protect them. Since, we basically do everything for them; they need not evolve into a stronger, more powerful species.

However, in the case of stray dogs, they have to fight for their survival. They have to fight with other dogs for food and shelter. Hence, survival of the fittest will apply here as the strongest dog gets to eat.

Hope this helps.

Please mark me as brainliest.

What is the name of the organelle in the leaves of plants that absorbs the sun's energy?.

Answers

Answer:

The name is chloroplasts

Explanation:

What happens when an action potential is produced with a signal that is stronger than threshold?

A)
weaker action potential generated

B)
no action potential generated

C)
action potential has same strength as threshold

D)
stronger action potential generated

Answers

Answer:

C) action potential has same strength as threshold

Explanation:

What happens when an action potential is produced with a signal that is stronger than threshold action potential has same strength as threshold

When an action potential is produced with a signal that is stronger than the threshold, the resulting action potential is typically stronger or more intense compared to a standard action potential. The answer is D) Stronger action potential generated.

The threshold is the minimum level of depolarization required to initiate an action potential in a neuron. If the signal surpasses the threshold, it means that the depolarization is greater than what is necessary to trigger the action potential.

When a stronger signal is received by the neuron, it results in a larger influx of ions (such as sodium) into the neuron, leading to a more pronounced depolarization of the cell membrane.

As a result, the action potential generated will have a higher amplitude or voltage compared to a regular action potential. Therefore, the answer is D) Stronger action potential generated.

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when plants are red flowers are crossed with plants with white flowers What proportion of The Offspring will have pink flowers

Answers

The pink flower donates the r allele and produces pink flowers in 50% of the offspring. The genotype for the pink flower is Rr and the genotype for the white flower is rr. This would lead to a 50% chance of the offspring having a phenotype of pink.

Higher concentration of water than the cell.

Answers

Water flows out the cell

Plz help this due at 10am! please and thank and SHOW WORK! must

Answers

Answer:

I don't know but Good luck

What is the function of parenchyma cells?

Answers

Answer:

help in storage and support

Select ALL the correct answers.
Which statements correctly describe mutations in gametes and mutations in somatic cells?
Mutations to somatic cells are transferred to daughter cells,
ODOS O
Mutations to gametes can be passed on to an organism's offspring,
Mutations to gametes do not involve changes
in the DNA.
Mutations to somatic cells are only present in the type of cell in which the mutation occurred.
Mutations to somatic cells will be inherited by an organism's offspring
Mutations to gametes are more likely to be beneficial than mutations to somatic cells.
Reset
Next

Answers

i just want lou bts my dad to

As a result of higher osmotic gradient outside, ____________ moves out of the thin descending limb of Loop of Henle

Answers

Answer:

is it water?

Explanation:

As a result of higher osmotic gradient outside, water moves out of the thin descending limb of Loop of Henle.

What is osmotic potential?

Osmotic potential is a component of water potential whose magnitude depends on the amount of solutes present in the solution, with osmotic pressure defined as the pressure necessary to prevent osmosis from occurring.

The loop of Henle (or nephr), in anatomy, is the segment of the nephron that comes just after the proximal convoluted tubule, being a U-shaped tubular structure, with a thick and a thin portion. That can be done with the osmotic potential.

See more about loop of Henle at brainly.com/question/13148548

Black hair (b) is dominant over brown hair (b) in guinea pigs. What is the genotype of a brown guinea pig?.

Answers

Answer:

the genotype of the brown guinea pig would be 'bb'

Explanation:

if it had black hair the possible genotypes would be 'Bb' or 'BB' because any genotype with a capital letter is dominant and brown hair is a recessive trait I'm assuming considering the question says "over"

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