An ion exchange column is the best option to purify a 75 kDa negatively charged tagged protein from a 120 kDa negatively charged protein based on their net charge difference.
Ion exchange columnAn ion exchange column would be the most suitable option to purify a 75 kDa negatively charged tagged protein from a 120 kDa negatively charged protein.
Ion exchange chromatography separates molecules based on their net charge. In this case, the negatively charged tagged protein will bind to the positively charged stationary phase of the column, while the larger negatively charged protein will flow through the column.
The bound protein can then be eluted from the column using a buffer with a high concentration of salt or a change in pH that reduces the binding affinity of the protein to the column.
Both Ni+2 agarose and G100 gel filtration columns are useful in protein purification, but they may not be suitable for separating two similarly charged proteins. Ni+2 agarose is typically used for purifying histidine-tagged proteins, while gel filtration is mainly used for size-based separation of proteins. However, if the 75 kDa protein has a histidine tag, then a Ni+2 agarose column could also be used for purification.
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how are indicator species used to assess pollution levels in the environment?
Answer:
By monitoring changes in the population of a specific indicator species, scientists may draw conclusions about changes in a specific pollutant's levels. In addition, this method of pollution measurement demonstrates the impact of pollution on vulnerable species.
Explanation:
Why do you think it's important for scientists to study plate tectonics? Explain.
Studying plate tectonics is important because it helps explain Earth's surface features, predict natural disasters, investigate Earth's history, explore natural resources, and understand global climate patterns. It is a multidisciplinary field that provides valuable insights into the dynamic nature of our planet.
Scientists study plate tectonics for several important reasons. Firstly, understanding plate tectonics helps scientists explain the formation of different features on Earth's surface, such as mountains, valleys, and ocean basins. By studying the movement and interactions of Earth's tectonic plates, scientists can better understand the processes that shape our planet.
Secondly, studying plate tectonics is crucial for predicting and understanding natural disasters. Earthquakes, volcanic eruptions, and tsunamis are all related to tectonic activity. By analyzing plate boundaries and identifying areas prone to seismic activity, scientists can provide early warnings and help communities prepare for potential disasters.
Furthermore, studying plate tectonics is essential for investigating Earth's history. The movement of tectonic plates over millions of years has shaped the continents and influenced the evolution of life on our planet. By examining the distribution of fossils and rock formations, scientists can reconstruct past tectonic events and gain insights into Earth's geological history.
Additionally, understanding plate tectonics is crucial for exploring natural resources. Many valuable minerals and energy resources are formed as a result of tectonic processes. By studying plate boundaries and their associated geological features, scientists can identify potential locations for mining and resource extraction.
Lastly, studying plate tectonics contributes to our understanding of global climate patterns. The movement of tectonic plates affects ocean currents, weather patterns, and the distribution of landmasses. By analyzing these interactions, scientists can better predict and understand climate change.
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What is the major thing happening to a cell during G1?
Answer:
During this stage new organelles are being synthesized (made), so the cell requires both structural proteins and enzymes, resulting in great amount of protein synthesis. Color the G1 phase green on the cell cycle drawing.
Explanation:
What brain region is responsible for coordinating and refining movement?
The diencephalon
The cerebrum
The brain stem
The cerebellum
Answer:
The cerebellum
Explanation:
Answer:
I hope this helps.
Explanation:
Each of the following is a density-dependent limiting factor EXCEPT:
- crowding
- predation
- competition
- disease
Answer:
predation
Explanation:
predation
I hop this answer is correct
Answer:
Disease
Explanation:
is ecosystem generally stable; how is that possible?
Answer:
Citation: Cleland, E. E. (2011) Biodiversity and Ecosystem Stability. ... traits, and species that share similar suites of traits are often categorized together into ... Biologically diverse communities are also more likely to contain species that confer
Explanation:
Which explanation best identifies the relationship between mitosis and
reproduction? *
A.Mitosis is a form of sexual reproduction because the daughter cells form from one
single parent cell.
B.Mitosis is a form of sexual reproduction because the daughter cells are genetically
different from the parent cell.
C.Mitosis is a form of asexual reproduction because the daughter cells form a new
nuclear membrane after reproduction.
D.Mitosis is a form of asexual reproduction because the daughter cells are genetically
identical to the parent cell.
Answer:
D.
Explanation:
Mitosis three main functions are growth and repair of cells, and asexual reproduction for the single celled organisms.
The daughter cells have same chromosomes/genetics.
Example: repair and growth of skin, red blood cells etc.
Answer:
It's D
Explanation:
Are the members of the third generation still carriers/affected by the trait? How many
percent are affected by the trait?
Answer:
When a genetic disorder is diagnosed in a family, family members often want to know the likelihood that they or their children will develop the condition. This can be difficult to predict in some cases because many factors influence a person's chances of developing a genetic condition. One important factor is how the condition is inherited. For example:
Autosomal dominant inheritance: A person affected by an autosomal dominant disorder has a 50 percent chance of passing the mutated gene to each child. The chance that a child will not inherit the mutated gene is also 50 percent. However, in some cases an autosomal dominant disorder results from a new (de novo) mutation that occurs during the formation of egg or sperm cells or early in embryonic development. In these cases, the child's parents are unaffected, but the child may pass on the condition to his or her own children.
How can homologous structures be used as evidence for shared common ancestry?
What type of protein is missing in the blue people of KY?
Answer:
four-part protein
Explanation:
The blue people of Troublesome Creek had methemoglobinemia, a metabolic condition affecting hemoglobin, the four-part protein that carries oxygen bound to an iron atom at each subunit's core.
Hope this helps:)
Answer:
The blue people of Troublesome Creek had methemoglobinemia, a metabolic condition affecting hemoglobin, the four-part protein that carries oxygen bound to an iron atom at each subunit's core.
Explanation:
PLEASE MARK ME AS BRAINLIEST
Describe the type of plate movement that is occurring where subduction zone volcanoes are found.
Answer:
Convergent boundary
Explanation:
It's when the plates move together and subduction occurs when one plate moves underneath another on a convergent boundary. Volcanoes are found there.
P.S. Brainliest, please?
The developement of fruit without pollination, resulting in seedless fruit is called __________.
A)
genetic engineering
B)
parthenocarpy
C)
biomagnification
D)
epiphysis
E)
browsing
Answer:
B) parthenocarpy
Explanation:
Parthenocarpy is the development of fruit without fertilization of seeds. Another biological mechanism stenospermacarpy also produces seedless fruits, but here the seeds are aborted while they are still small. Some plants require pollination or stimulation for parthenocarpy.
:)
Please help due in 10 minutes!
Explain how genetic drift of alleles in a small population- and- describe 2 real world examples of genetic drift (I.e. The Founder Effect and The Bottleneck Effect)
Answer:A small population is formed with a larger population.
Explanation:The population don’t represent the genetic diversity’s of the original
Population, and there smaller size mean they may experience strong drift of generations.
Why do cells need to transport molecules?
Answer:
its the cell membrane
Explanation:
heres gogle support but i also did this in class
What type of materials need to be transported out of the cell?
Water, carbon dioxide, and oxygen are among the few simple molecules that can cross the cell membrane by diffusion (or a type of diffusion known as osmosis ). Diffusion is one principle method of movement of substances within cells, as well as the method for essential small molecules to cross the cell membrane.
Answer:
porque le permite expulsar de su interior los desechos del metabolismo, también el movimiento de sustancias que sintetiza como hormonas.
Explanation:
A student examining an unknown cell under a microscope notes it has a cell wall, cytoplasm, and genetic information contained within a nuclear membrane. Which inference would be correct?
Answer:
It is a eukaryotic cell
Explanation:
Eukaryotic cells have membrane-bound organelles (in this case the cell has a nuclear membrane with genetic information).
Bacteria, archaea, fungi, plants, and algae have a cell wall
List the four steps of mitosis in order. Describe what is happening during each phase.
Answer:
The four stages of mitosis are known as prophase, metaphase, anaphase, telophase. Additionally, we'll mention three other intermediary stages (interphase, prometaphase, and cytokinesis) that play a role in mitosis. During the four phases of mitosis, nuclear division occurs in order for one cell to split into two.
Explanation:
Which diagram shows photosynthesis with the correct reactants and products?
answer:
B
explain:
i did the test:))
Answer:c
Explanation:
How could physical barriers, such as a river or rift, lead to allopatric speciation
Answer:
This barrier prevents the individuals on one side from reproducing with the individuals on the other.
Explanation:
Hope this helps
Which of these has a warming effect on Earth? O A. More evaporation of water due to warmer temperatures causes low, thick clouds to form. O B. More evaporation of water due to warmer temperatures increases water vapor, forming high, thin clouds. C. Earth's oceans absorb carbon dioxide from the atmosphere D. Rising carbon dioxide levels spur plant growth, causing increased carbon dioxide absorption.
Answer:
A
Explanation:
Thick clouds can trap lots of heat. This has a warming effect on earth.
Answer:
B.. More evaporation due to warmer temperatures increases water vapor, forming high, thin clouds
Explanation:
Thin clouds don't reflect as much sunlight as low, thick clouds do. Because of that more sunlight enters the atmosphere and increases global warming
If water is polar, state a liquid that you think is nonpolar, and justify your answer
Welp: That moment I knew I didn't pay attention when I should have
Answer:
a series circuit has a 2 Ω (ohm) resistor, a 5 Ω resistor, and a 7 Ω resistor. The total resistance of the circuit is 2 + 5 + 7 = 14 Ω.
Explanation:
Not very good for you
Answer:
lol
Explanation:
Yeah you need to start paying attention in class
would you expect a plant to produce more oxygen on a cloudy day or a sunny day? explain
Answer:
You would expect a plant to produce more oxygen on a sunny day because there are more sun rays available to plants so photosynthesis is easier to maintain, as well as oxygen production.
Which of the following IS
an example of reproductive isolation?
O two populations
of finches that cannot produce viable offspring
O two populations of finches that have different feather colors
• two populations of salamanders that live in different lakes
O two populations of salamanders that
have different tail lengths
An example of reproductive isolation is two populations of salamanders that live in different lakes. Thus, the correct option is C.
What is Reproductive isolation?Reproductive isolation may be defined as the inability of a species to interbreed successfully with related species members due to isolating mechanisms.
Reproductive isolation may leads to speciation. A single specie scan generates multiple species via reproductive isolation. The members of the same species undergo reproductive isolation due to several isolating mechanisms.
The isolating mechanisms include geographical, behavioral, physiological, or genetic barriers.
Therefore, the correct option for this question is C.
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All of the following are types of somatoform disorders except __________.
A.
conversion disorder
B.
obsessive-compulsive disorder
C.
somatization disorder
D.
hypochondrias
Answer:
B.
obsessive-compulsive disorder
Explanation:
The types of somatoform disorders does not include the obsessive-compulsive disorder.
What is somatoform disorders?Somatic symptom disorder (SSD should be called as the somatization disorder or somatoform disorder. It is the form of mental illness that leads into one or more symptoms such as pain.
Therefore, it include the conversion disorder, somatization disorder, hypochondrias
Hence, the option b is correct.
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HELPPPPP ASAPPPPP!!!!!!
How do you think the organisms interact with each other?
Answer:
There are four main types of species interactions that occur between organisms in an ecosystem: Predation, parasitism and herbivory - In these interactions, one organism benefits while the other is negatively affected. * Competition - Both organisms are negatively affected in some way due to their interactions. Individual organisms live together in an ecosystem and depend on one another. Some organisms can make their own food, and other organisms have to get their food by eating other organisms. An organism that must obtain their nutrients by eating (consuming) other organisms is called a consumer, or a heterotroph.
I hoped this helped you out some~ <3
-Dream
PLZ HELP!! Which expels solid waste from the body? A. Liver. B. Large intestine. C. Pancreas. D. Anus
Answer:
anus LKEJKEGWHRJK
Explanation:
Answer:
I think it is D) Anus
Explanation:
Solid waste leaves the body through the anus
Hope that helps
Determine the type of air mass that typically plays a role in the weather in your area. Describe the
type of weather that commonly is associated with the air mass and where the air mass originates. Describe the seasons in your area which result from that air mass.
Answer:
There are four categories for air masses: arctic, tropical, polar and equatorial. Arctic air masses form in the Arctic region and are very cold.
how are organisms impacted by the changing landscape
Answer:
Animals can be impacted in a variety of ways, one example being that humans continue to expand their territory they are taking homes and resources to organisms that inhabit that land, this can lead to population decreases as the organism will be forced into a new environment that they are not adapted to.
__________ is the principle of dealing with environmental problems without discriminating against people based upon socioeconomic status, race, or ethnicity.
A)
Industrial ecology
B)
Sustainable development
C)
Materials management
D)
Environmental justice
E)
Monitoring
Answer: The correct option is D.
Environmental justice
Explanation:
This is because environmental justice is the unbiased treatment of people or non discrimination of people of different color, race, ethinc group, status, income with respect to the development of the environment by implementing different environmental laws, polices, regulations e.t.c for the safety of all.