Describe the process of cloning from start to finish.

Answers

Answer 1

Answer:

1. Isolation of donor DNA fragment or gene.

2. Selection of suitable vector.

3. Incorporation of donor DNA fragment into the vector.

4. Transformation of recombinant vector into a suitable host cell.

5. Isolation of recombinant host cell.

Answer 2

Answer:

Explanation:

In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) ...

Related Questions

HELP! whoever does these gets brainliest!!

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Answer:

blood crime scene arson murder footprint

PLEASE I NEED HELP ASAP :(

Answers

Answer:

b

Explanation:

the rest just wont help when you are trying to identify them

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Explanation:

not interested............

Unlike photosynthesis, cellular respiration occurs in

a
plants, animals, protists, fungi and bacteria
b
plant cells only
c
all organisms except plants
d
animal cells only

Answers

Answer:

A

Explanation:

All organisms do cellular respiration as it is essential for life.

Water is transpired through stomata, but carbon dioxide also must pass through stomata into a leaf for photosynthesis to occur. There is evidence that the level of carbon dioxide in the atmosphere has not always been the same over the history of life on Earth. Explain how the presence of a higher or lower concentration of atmospheric carbon dioxide would impact the evolution of stomata density in plants.

Answers

Answer:

Escribir en español, por favor.?

During those high-carbon-dioxide times, plants that produce fewer stomata,

when carbon dioxide levels are low, plants need many stomata in order to scrape enough carbon dioxide to survive.

How does carbon dioxide affect water uptake?

Plant water use, an increase in atmospheric CO2 means that stomata, or the pores on leaves that exchange gases between the leaf and the atmosphere, do not need to open.

Thus,  carbon dioxide levels are low, need many stomata in order to scrape enough carbon dioxide to survive.

To learn more about carbon dioxide click here:

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Greenhouse gases are important to climate change because they

A. Are the best way to measure if the temperature is changing over time

B. help to trap energy from the sun in the Earth's atmosphere

C.help to block the radiation of the sun from harming Earth

D. are the main reason that the earth becomes warmer and cooler

Answers

Answer:

D

Explanation:

well, without the greenhouse gases the earth's temperature will drop drastically but too much of it will cause the earth's temperature to rise

Explanation:

Answer- help to trap energy from the sun in the Earth's atmosphere.

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Answers

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Answer: Prokaryotic

Explanation:

I hope this helped!

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Answer:

the correct answer choice is c.

Explanation:

prokaryotic cells lack both a nucleus and membrane-bound organelles.

EARTH/ENVIRONMENTAL SCIENCE
RELEASED ITEMS
18
Which is an effect of lower levels of solar radiation striking Earth?
A
increased incidences of skin cancer
B
decreased rates of photosynthesis
С
increased phytoplankton activity
D
decreased carbon dioxide levels
plate boundary force

Answers

B is the answer of the question

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ATP is made during the Krebs cycle between two molecules

A.Succinyl COA
B.Fumarate
C.Malate
D.Alpha Ketoglutarate
E.Succinate

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Answer:

The answer should be A,if not than sorry!

correct answers only please!

Answers

It’s B I’m 99% sure lol

please help asap and I’ll give brainiest!

Answers

Answer:

I think so A is the answer

Explanation:

changes in Geology over Time ... relative age – a rock layer's age compared to other rock layers ... Fossils give clues on how Earth may ... weather. ▫This helps us to infer that West. Virginia and Pennsylvania were both ... in the area. ... Many fish. Silurian 438 to 408 mya. First insects, vascular plants on land.

i hope my answer is correct and would help a little bit.

List the steps of DNA replication from start to finish
- Two identical strands of DNA are created
- The DNA strands separate
- Nucleotides are added to the bases
- Individual bases are exposed

Answers

Answer:

2,4,3,1..............

which is an abiotic factor that can be found in a rain forest ecosystem

Answers

Answer:

Climatic factor.

Explanation:

Climatic factor is an abiotic factor that can be found in rainforest ecosystem. The climate along with the nature of soil and water is an area determine the type of organism found in the area,In fact both living and nonliving component influence each other.Among the abiotic factors the most important are climatic and edaphic factors.The climatic factors include air, water, light, temperature and humidity the edaphic factor refer to soil.

Answer:

oxygen

Explanation:

trust me just took it

HELP ME PLEASE PLEASE PLEASE 20 POINTS AND BRAINLIEST

Answers

Answer:

ITS A(the endiplasmin reticulum)

Explanation:

how does the structure of simple squamous cells allow it does to its function?

Answers

Answer:

Structure of the simple squamous epithelium

The cells appear like scales that provide a smooth low-friction surface over which fluids can move quickly. Membrane carrier proteins are found in this layer that function to facilitate the movement of ions, gases, small molecules, or water through the cells.

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Answers

25% because colorblindness is sex linked

How does the environment influence populations of organisms over multiple
generations?

Answers

Answer: Species can change over time in response to changes in environmental conditions through adaptation by natural selection acting over generations. ... Evolution results primarily from genetic variation of individuals in a species, competition for resources, and proliferation of organisms better able to survive and reproduce.

Explanation:

How does the skeletal system interact with the nervous system?
A
the nervous system supplies the blood and oxygen to developing bones

B
the nervous system supplies nutrients and minerals to developing bones

C
the skeletal system controls the messages sent to the brain and spinal cord

D
the skeletal system provides protection for the brain and spinal cord

Answers

Answer:

D

Explanation:

Bones provide calcium that is essential for the proper functioning of the nervous system.

Answer:

c is the answer it sends messages

What is a density dependent limiting factor that affects population size?
-Drought
-Climate
-Natural Disasters
-Disease

Answers

Answer:

Disease

Explanation:

In Ecology, certain factors that affect the size of a population can either be dependent on size or not. Density-dependent factors are those factors that affect population of organisms in dependence of how dense the population is. Examples of these density dependent factors are diseases, predation etc.

For example, a certain disease will spread faster among a population of organisms whose size is dense but slower in a scarcely densed population. Hence, disease as a factor is dependent on population size. Note that; Drought, Climate, and Natural Disasters will wipe out a population irrespective of its size.

Answer:

Disease

Explanation:

1
What is the original source of energy for most ecosystems?

A.grass
B.humans
C.the sun
D.heat

Answers

Answer:

C. The sun

Explanation:

The sun provides energy for the plant to do photosynthesis. Since all animal species depend on plants qnd the sun helps the plants, the sun is the original source of energy.

Answer:

C,The sun

Explanation:

the vast majority of energy in food webs originates from the sun

Which of the following is a biotic in an ecosystem?

A. Wind

B. Tree leaves

C. Small rocks

D. Light from the sun

Answers

Answer:

tree leavessssssssssssssssssss

Answer:

B. tree leaves

Explanation:

A biotic is something in an ecosystem that are components of a community such as plants and animals. Leaves can be consumed by animals which makes them biotic. Temperature and climate are NOT biotic.

Have a good day.

A virus infects a lung cell and begins replication. The lung cell releases interferon into the extracellular environment where it can be picked up by other nearby cells. As a result, the other nearby cells slow down their protein-making machinery to slow down viral replication should they become infected. This is an example of what kind of communication

Answers

Answer: The options are not given but it is gotten from another websites and here are the options.

A.Cell-to-cell (membrane molecule) recognition

B. Cell junctions

C. Paracrine signaling

d. Synaptic signaling

The correct option is C.

Paracrine signaling.

Explanation:

This is because in paracrine signaling cell communicate with other near by cells through chemical Messengers. It is a form of cell to cell communication in which a cell produced signal and transfer it to other neighbouring cells whereby the neighbouring cells alter or change their behavior to counter the effect of the signal. From the question, the lung cells send signals to neighbouring cells and this alter their behavior by slowering down protein making machinery.

29.
Use the diagram below to answer questions 29 and 30.
Use Note-taking 22-6 to help you identify the five major root structures on the drawing of a
dicot root.
А A.
B
B.
с
C.
D.
D
E.
E

Answers

Answer:

The diagram represents the process of enlarging a rectangle using a scale factor of 3. The width of the original rectangle must be:

9 in.

11 in.

12 in.

17 in.

Explanation:

Kahlia was given antibiotics and told to rest. Bored by spending all day resting, Kahlia decided to learn more about her respiratory system. Select all statements that are true of the respiratory system.

a. Both the alveolar wall and capillary wall contribute to the respiratory membrane.
b. All structures of the respiratory system have cilia to move mucus.
c. Mucus-secreting cells are found throughout the structures of the conducting and respiratory zone.
d. The alveoli have a simple squamous epithelium to allow for diffusion of oxygen and carbon dioxide across the respiratory membrane.
e. All structures of the conducting and respiratory zone are lined by a serous membrane.

Answers

Answer:

The correct statements are: A and D.

Explanation:

a. Both the alveolar wall and capillary wall contribute to the respiratory membrane.

The respiratory membrane is a more or less homogeneous surface composed of a set of structures that gases must cross between the alveolus and the pulmonary capillary. When blood reaches the lungs it has a high CO2 content and a low O2 content. O2 passes by diffusion through the alveolar walls and capillaries into the blood. There it is transported by hemoglobin, which will take it to the cells of the body where, by the same diffusion process, it will pass inside them for later use.

d. The alveoli have a simple squamous epithelium to allow for diffusion of oxygen and carbon dioxide across the respiratory membrane.

The function of the lungs is to carry out the gas exchange with the blood, for this the alveoli are in close contact with the capillaries. The simple flat squamous epithelium lines the alveoli of the lungs, they are flattened and irregular cells in a single cell layer that allow the easy diffusion of water, gases and other substances. In the alveoli there is the passage of oxygen from the air to the blood and the passage of carbon dioxide from the blood to the air.

In normal cells, stathmin is inactivated by phosphorylation at the start of M phase. Phosphatases remove these phosphates as the cell transitions from M phase to G1. What enzyme is likely to be responsible for phosphorylating stathmin during M phase

Answers

Answer:

The enzyme that is responsible for this is the cyclin dependent kinase subunit of mpf

Explanation:

This enzyme is also known as cyclin dependent kinase subunit, CDKs. they are protein kinases and can function as cell cycle regulators. They have the power to modify different protein substrates that are useful for the progression of cell cycle. CDKs are inhibitors hence stathmin is inactivated by phosphorylation. They help to control cell divisions. DNA should not be damaged for cell to move from G₁ to the S phase.

The enzyme that is likely to be responsible for phosphorylating stathmin during M phase is the cyclin dependent kinase.

Cyclin-dependent kinases:

Cyclin-dependent kinases (CDKs) are protein kinases characterized by needing a separate subunit - a cyclin - that provides domains essential for enzymatic activity. CDKs play important roles in the control of cell division and modulate transcription in response to several extra- and intracellular cues. CDKs are inhibitors hence stathmin is inactivated by phosphorylation. They help to control cell divisions. DNA should not be damaged for cell to move from G₁ to the S phase.Full kinase activity requires an activating phosphorylation on a threonine adjacent to the CDK's active site.

Learn more:

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Am I correct? Tell me

Answers

Answer:

Yep, at least from what I know.

Correct

Explanation:

Answer:

I believe you are correct.

Explanation:

Air masses form over regions where the air is stable for a long enough time. The air takes on the characteristics of the region. Air masses move when they are pushed by high level winds.

What is one of the causes of daily variations in temperature on a planet's surface?
OA.
the speed of a planet's rotation
OB.
the rotation of a planet's moons
O C.
the time it takes for a revolution around the sun
OD.
the tidal force on the oceans

Answers

Answer:

D

Explanation:

i am sorry if this is wrong

Answer:

A. the speed of a planet's rotation

Explanation:

Which of the following is not a normal body response to heat?
Select one:
a. dilation of peripheral blood vessels
b. increased sweating
C. decreased rate of muscle activity
d. increased retention of blood in the core region
e. increased water loss

Answers

Answer:

c. decrease rate of muscle activity

From DNA to protein involves which series of events?Which of the following correctly describes the locations of transcription and translation within a eukaryotic cell?

Answers

Answer:

DNA istranscribed in the nucleus, then the mRNAtranscript is transported to the cytoplasm to be translated into protein.

Explanation:

I hope that this is what you were looking for

Explain the different ages/stages of bone development from fertilisation to adulthood.

Answers

Answer:

adolescent

Explanation:

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